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Class I myosins regulate protrusive forces at the actin-membrane interface

Award Number: R01GM138652
ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 07/06/2021
PERIOD OF PERFORMANCE END DATE: 04/30/2025

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Class I myosins regulate protrusive forces at the actin-membrane interface - In this project, we will dissect the roles of class I myosins, molecular motors that bind actin filaments through their motor domains and plasma membrane through their tail domains, in actin-mediated cell shape changes. We will test the hypothesis that myosin I motor activity promotes assembly of branched actin networks at the sites of membrane deformation, including endocytic vesicles, phagocytic cups, and podosomes. The proposed work will use two model systems: fission yeast, where myosin I is required for endocytosis, and murine macrophages, which rely on myosin I for phagocytosis and cell migration. In aim 1, we will determine how myosin motor activity contributes to endocytic actin patch assembly and internalization in yeast. We will use the genetically tractable yeast system to express mutant myosin from the endogenous myosin locus and apply molecule counting techniques, which are well established in the yeast endocytosis system, to measure the rates of actin assembly. In aim 2, we will test the role of myosin motor activity in actin and membrane dynamics during phagocytosis and cell migration. These studies will take advantage of the genetically modified mice lacking class I myosins that are available in our labs. In aim 3, we will determine how myosin-membrane interactions are regulated to contribute to myosin functions in actin assembly and immune response. The proposed work will shed light on the molecular mechanisms driving actin- dependent membrane deformation.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2024 ( Subtotal = $534,028 )
2024	2024	RESEARCH FOUNDATION FOR THE STATE UNIVERSITY OF NEW YORK, THE	750 E ADAMS ST	SYRACUSE	NY	13210	ONONDAGA	USA	Biomedical Research and Research Training	001	4	7/23/2024	NON-COMPETING CONTINUATION	$53,402
2024	2024	RESEARCH FOUNDATION FOR THE STATE UNIVERSITY OF NEW YORK, THE	750 E ADAMS ST	SYRACUSE	NY	13210	ONONDAGA	USA	Biomedical Research and Research Training	000	4	4/12/2024	NON-COMPETING CONTINUATION	$480,626
														Subtotal = $534,028

Issue Date FY: 2023 ( Subtotal = $534,028 )
2023	2023	RESEARCH FOUNDATION FOR THE STATE UNIVERSITY OF NEW YORK, THE	750 E ADAMS ST	SYRACUSE	NY	13210	ONONDAGA	USA	Biomedical Research and Research Training	000	3	5/2/2023	NON-COMPETING CONTINUATION	$534,028
														Subtotal = $534,028

Issue Date FY: 2022 ( Subtotal = $784,028 )
2022	2022	RESEARCH FOUNDATION FOR THE STATE UNIVERSITY OF NEW YORK, THE	750 E ADAMS ST	SYRACUSE	NY	13210	ONONDAGA	USA	Biomedical Research and Research Training	000	2	4/20/2022	NON-COMPETING CONTINUATION	$534,028
2022	2022	RESEARCH FOUNDATION FOR THE STATE UNIVERSITY OF NEW YORK, THE	750 E ADAMS ST	SYRACUSE	NY	13210	ONONDAGA	USA	Biomedical Research and Research Training	001	2	8/12/2022	SUPPLEMENT FOR EXPANSION	$250,000
														Subtotal = $784,028

Issue Date FY: 2021 ( Subtotal = $541,028 )
2021	2021	RESEARCH FOUNDATION FOR THE STATE UNIVERSITY OF NEW YORK, THE	750 E ADAMS ST	SYRACUSE	NY	13210	ONONDAGA	USA	Biomedical Research and Research Training	000	1	7/6/2021	NEW	$541,028
														Subtotal = $541,028

Grand Total All Awards = $2,393,112

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Class I myosins regulate protrusive forces at the actin-membrane interface

Award Number: R01GM138652

ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 07/06/2021

PERIOD OF PERFORMANCE END DATE: 04/30/2025

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