Friday, October 3, 2025 10/3/2025

Development of solid-state nanopore technology for improved glycosaminoglycan analytics

Award Number: R01GM134226
ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 09/30/2020
PERIOD OF PERFORMANCE END DATE: 06/30/2028

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Development of solid-state nanopore technology for improved glycosaminoglycan analytics - Along with nucleic acids and proteins, glycosaminoglycans (GAGs) are considered a foundational building block of biology. However, unlike their more widely-studied counterparts, the tools for analyzing GAGs are relatively lacking. In our prior R01, we developed solid-state (SS-) nanopore technology for GAG analysis, focusing on the key molecule hyaluronan (or hyaluronic acid, HA) and establishing a method for determining molecular weight (MW) distribution from a small sample amenable to physiological levels. This has allowed new investigations into the size-function relationship of HA. The focus of this project will be to increase the value of the SS-nanopore platform both in terms of HA measurement and as a general GAG analytical tool. In the first Aim, we will first implement a strategy for HA quantification that can be coupled with our existing MW assessment to render a more versatile technology. HA abundance is an independent identified biomarker in several disease states, making it valuable in combination with size distribution. Then, we will establish a microfluidic infrastructure to enable HA extraction from biological matrices and subsequent SS-nanopore analysis to be performed in a self-contained format, improving the accessibility of the approach to other laboratories. In the second Aim, we will first perform pilot measurements toward an improved reagent for extraction of HA coupled to protein markers of inflammation (heavy chains), with the goal of targeting a single protein isoform and establishing feasibility for selection between the multiple known isoforms that are thought to have independent functions. Finally, we will extend the SS-nanopore analytical approach to other GAGs, focusing on the important molecules heparan sulfate and chondroitin sulfate and integrating methods for assessing sulfation levels. Overall, this project will result in a more powerful technology that will further the field of glycobiology. 2.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2025 ( Subtotal = $369,227 )
2025	2025	WAKE FOREST UNIVERSITY HEALTH SCIENCES	MEDICAL CENTER BLVD	WINSTON SALEM	NC	27157	FORSYTH	USA	Biomedical Research and Research Training	002	6	9/9/2025	NON-COMPETING CONTINUATION	$369,227
2025	2024	WAKE FOREST UNIVERSITY HEALTH SCIENCES	MEDICAL CENTER BLVD	WINSTON SALEM	NC	27157	FORSYTH	USA	Biomedical Research and Research Training	001	5	4/30/2025	COMPETING CONTINUATION	$43,476
2025	2023	WAKE FOREST UNIVERSITY HEALTH SCIENCES	MEDICAL CENTER BLVD	WINSTON SALEM	NC	27157	FORSYTH	USA	Biomedical Research and Research Training	000	4	4/30/2025	NON-COMPETING CONTINUATION	-$43,476
														Subtotal = $369,227

Issue Date FY: 2024 ( Subtotal = $354,963 )
2024	2024	WAKE FOREST UNIVERSITY HEALTH SCIENCES	MEDICAL CENTER BLVD	WINSTON SALEM	NC	27157	FORSYTH	USA	Biomedical Research and Research Training	001	5	9/19/2024	COMPETING CONTINUATION	$354,963
2024	2023	WAKE FOREST UNIVERSITY HEALTH SCIENCES	MEDICAL CENTER BLVD	WINSTON SALEM	NC	27157	FORSYTH	USA	Biomedical Research and Research Training	000	4	9/18/2024	NON-COMPETING CONTINUATION	$0
														Subtotal = $354,963

Issue Date FY: 2023 ( Subtotal = $305,774 )
2023	2023	WAKE FOREST UNIVERSITY HEALTH SCIENCES	MEDICAL CENTER BLVD	WINSTON SALEM	NC	27157	FORSYTH	USA	Biomedical Research and Research Training	000	4	7/7/2023	NON-COMPETING CONTINUATION	$305,774
														Subtotal = $305,774

Issue Date FY: 2022 ( Subtotal = $305,885 )
2022	2022	WAKE FOREST UNIVERSITY HEALTH SCIENCES	MEDICAL CENTER BLVD	WINSTON SALEM	NC	27157	FORSYTH	USA	Biomedical Research and Research Training	000	3	8/30/2022	NON-COMPETING CONTINUATION	$305,885
														Subtotal = $305,885

Issue Date FY: 2021 ( Subtotal = $306,199 )
2021	2021	WAKE FOREST UNIVERSITY HEALTH SCIENCES	MEDICAL CENTER BLVD	WINSTON SALEM	NC	27157	FORSYTH	USA	Biomedical Research and Research Training	000	2	7/15/2021	NON-COMPETING CONTINUATION	$306,199
														Subtotal = $306,199

Issue Date FY: 2020 ( Subtotal = $312,723 )
2020	2020	WAKE FOREST UNIVERSITY HEALTH SCIENCES	MEDICAL CENTER BLVD	WINSTON SALEM	NC	27157	FORSYTH	USA	Biomedical Research and Research Training	000	1	9/5/2020	NEW	$312,723
														Subtotal = $312,723

Grand Total All Awards = $1,954,771

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Development of solid-state nanopore technology for improved glycosaminoglycan analytics

Award Number: R01GM134226

ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 09/30/2020

PERIOD OF PERFORMANCE END DATE: 06/30/2028

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