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Genetic and biochemical dissection of anterograde signaling for controlling plastid transcription

Award Number: R01GM132765
ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 02/07/2020
PERIOD OF PERFORMANCE END DATE: 05/31/2028

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Genetic and biochemical dissection of anterograde signaling for controlling plastid transcription - Abstract The control of organellar gene expression is critical for the cellular programming of all eukaryotic organisms. While perturbing mitochondrial gene expression leads to human pathologies, including cancer, altering plastid gene expression can kill plants. However, the cell signaling mechanisms that control organellar gene expression remain poorly understood. The long-term goal of the PI’s laboratory is to utilize the light-induced plastid differentiation into photosynthetically active chloroplasts in Arabidopsis as a genetic model to interrogate cell signaling mechanisms for controlling organellar gene expression. The current data support the central hypothesis that the red and far-red photoreceptor phytochrome B promotes the degradation of a small family of phytochrome-interacting basic/helix-loop-helix transcription factors in the nucleus to generate nucleus-to-plastid (anterograde) signals that trigger the assembly and activation of the multisubunit, bacterial-type plastid RNA polymerase for transcribing plastid photosynthesis genes. Here the PI propose to utilize a combination of molecular genetics, biochemistry, and genomics approaches to (1) determine the mechanism initiating anterograde signaling in the nucleus, (2) determine the mechanism of the assembly of the multisubunit plastid RNA polymerase complex, and (3) determine the mechanism activating the plastid RNA polymerase. The proposed research is innovative because it utilizes photoreceptor signaling and chloroplast biogenesis in Arabidopsis as a genetic model to elucidate a previously uncharacterized anterograde signaling pathway. The PI has developed new forward genetic approaches and biochemical assays and identified critical components that define the framework of anterograde signaling. The proposed research is significant, because it is expected to uncover the light signaling mechanism for initiating chloroplast biogenesis - a long-standing gap in our knowledge of plant light signaling and the regulation of photosynthesis. Because the control of transcription in plastids shares intrinsic similarities with that in mitochondria, what we learn in the plastid model is expected to enhance the understanding of the general principles of cell signaling mechanisms in controlling organellar gene expression, including the regulation of mitochondrial gene expression, and therefore, will ultimately contribute to the understanding of the mechanisms underlying the misregulations of mitochondrial gene expression in human diseases.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2025 ( Subtotal = $319,912 )
2025	2025	REGENTS OF THE UNIVERSITY OF CALIFORNIA AT RIVERSIDE	200 UNIVERSTY OFC BUILDING	RIVERSIDE	CA	92521	RIVERSIDE	USA	Biomedical Research and Research Training	001	6	4/25/2025	NON-COMPETING CONTINUATION	$319,912
2025	2023	REGENTS OF THE UNIVERSITY OF CALIFORNIA AT RIVERSIDE	200 UNIVERSTY OFC BUILDING	RIVERSIDE	CA	92521	RIVERSIDE	USA	Biomedical Research and Research Training	000	4	3/20/2025	NON-COMPETING CONTINUATION	$0
														Subtotal = $319,912

Issue Date FY: 2024 ( Subtotal = $319,963 )
2024	2024	REGENTS OF THE UNIVERSITY OF CALIFORNIA AT RIVERSIDE	200 UNIVERSTY OFC BUILDING	RIVERSIDE	CA	92521	RIVERSIDE	USA	Biomedical Research and Research Training	002	5	7/23/2024	COMPETING CONTINUATION	$3,964
2024	2024	REGENTS OF THE UNIVERSITY OF CALIFORNIA AT RIVERSIDE	200 UNIVERSTY OFC BUILDING	RIVERSIDE	CA	92521	RIVERSIDE	USA	Biomedical Research and Research Training	001	5	7/9/2024	COMPETING CONTINUATION	$315,999
2024	2023	REGENTS OF THE UNIVERSITY OF CALIFORNIA AT RIVERSIDE	200 UNIVERSTY OFC BUILDING	RIVERSIDE	CA	92521	RIVERSIDE	USA	Biomedical Research and Research Training	000	4	7/9/2024	NON-COMPETING CONTINUATION	$0
														Subtotal = $319,963

Issue Date FY: 2023 ( Subtotal = $302,577 )
2023	2023	REGENTS OF THE UNIVERSITY OF CALIFORNIA AT RIVERSIDE	200 UNIVERSTY OFC BUILDING	RIVERSIDE	CA	92521	RIVERSIDE	USA	Biomedical Research and Research Training	001	4	2/22/2023	NON-COMPETING CONTINUATION	$30,257
2023	2023	REGENTS OF THE UNIVERSITY OF CALIFORNIA AT RIVERSIDE	200 UNIVERSTY OFC BUILDING	RIVERSIDE	CA	92521	RIVERSIDE	USA	Biomedical Research and Research Training	000	4	12/14/2022	NON-COMPETING CONTINUATION	$272,320
														Subtotal = $302,577

Issue Date FY: 2022 ( Subtotal = $363,804 )
2022	2022	REGENTS OF THE UNIVERSITY OF CALIFORNIA AT RIVERSIDE	900 UNIVERSITY AVE	RIVERSIDE	CA	92521	RIVERSIDE	USA	Biomedical Research and Research Training	001	3	6/20/2022	SUPPLEMENT FOR EXPANSION	$60,894
2022	2022	REGENTS OF THE UNIVERSITY OF CALIFORNIA AT RIVERSIDE	900 UNIVERSITY AVE	RIVERSIDE	CA	92521	RIVERSIDE	USA	Biomedical Research and Research Training	000	3	12/6/2021	NON-COMPETING CONTINUATION	$272,619
2022	2022	REGENTS OF THE UNIVERSITY OF CALIFORNIA AT RIVERSIDE	900 UNIVERSITY AVE	RIVERSIDE	CA	92521	RIVERSIDE	USA	Biomedical Research and Research Training	002	3	7/27/2022	NON-COMPETING CONTINUATION	$30,291
														Subtotal = $363,804

Issue Date FY: 2021 ( Subtotal = $357,759 )
2021	2021	REGENTS OF THE UNIVERSITY OF CALIFORNIA AT RIVERSIDE	200 UNIVERSTY OFFICE BLDG	RIVERSIDE	CA	92521	RIVERSIDE	USA	Biomedical Research and Research Training	001	2	3/15/2021	NON-COMPETING CONTINUATION	$30,064
2021	2021	REGENTS OF THE UNIVERSITY OF CALIFORNIA AT RIVERSIDE	200 UNIVERSTY OFFICE BLDG	RIVERSIDE	CA	92521	RIVERSIDE	USA	Biomedical Research and Research Training	002	2	9/20/2021	SUPPLEMENT FOR EXPANSION	$57,123
2021	2021	REGENTS OF THE UNIVERSITY OF CALIFORNIA AT RIVERSIDE	200 UNIVERSTY OFFICE BLDG	RIVERSIDE	CA	92521	RIVERSIDE	USA	Biomedical Research and Research Training	000	2	12/23/2020	NON-COMPETING CONTINUATION	$270,572
														Subtotal = $357,759

Issue Date FY: 2020 ( Subtotal = $301,041 )
2020	2020	REGENTS OF THE UNIVERSITY OF CALIFORNIA AT RIVERSIDE	200 UNIVERSTY OFFICE BLDG	RIVERSIDE	CA	92521	RIVERSIDE	USA	Biomedical Research and Research Training	000	1	2/7/2020	NEW	$301,041
														Subtotal = $301,041

Grand Total All Awards = $1,965,056

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Genetic and biochemical dissection of anterograde signaling for controlling plastid transcription

Award Number: R01GM132765

ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 02/07/2020

PERIOD OF PERFORMANCE END DATE: 05/31/2028

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