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Replacement of Widefield Imaging System

Award Number: R01GM125800
ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 01/22/2018
PERIOD OF PERFORMANCE END DATE: 12/31/2022

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Replacement of Widefield Imaging System - PROJECT SUMMARY The central aim of the parent grant is to identify roles of the proteasome along meiotic chromosomes. The 26S proteasome is the main site of protein degradation in all eukaryotes. It is a compartmentalized, multicomponent protease that resides both in the cytoplasm and the nucleus. Cytoplasmic proteasome functions in eliminating regulatory and misfolded proteins have been recognized for a long time. Functions of the proteasome in the nucleus are much less understood. We recently discovered that during meiosis I, the 26S proteasome is recruited in an evolutionarily conserved manner to chromosomes where it controls homolog pairing, synapsis and recombination. Using a combination of high-resolution microscopy, genetic and proteomic approaches, we are pursuing three aims that build on this discovery: First, we are using tightly controlled conditional alleles to systematically characterize roles during meiosis of different proteasome components. Second, we are identifying substrates of the proteasome relevant to chromosome pairing and recombination, providing insights into a class of molecules that need to be degraded to ensure normal meiotic progression. Third, we are identifying determinants for proteasome recruitment to chromosomes. Meiotic chromosome segregation defects in absence of a functional proteasome emphasize the importance for reproductive health of a better understanding of chromosome- associated proteolysis. Our analysis is also relevant to cancer therapy where proteasome inhibitors have become increasingly important. Many of our approaches involve high resolution and live-cell imaging in the model eukaryote budding yeast. In this supplement proposal, we are requesting funds for an advanced high-resolution, live imaging system as replacement for our aging current microscope. Our current system has given us valuable insights but has become unreliable and is near the end of its lifetime. A state-of-the- art imaging system will enable us to maintain and further enhance a high impact research program into mechanisms of chromosome transmission.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2024 ( Subtotal = -$1,961 )
2024	2021	CLEVELAND STATE UNIVERSITY	2121 EUCLID AVE	CLEVELAND	OH	44115	CUYAHOGA	USA	Biomedical Research and Research Training	001	4	10/16/2023	SUPPLEMENT FOR EXPANSION	-$663
2024	2021	CLEVELAND STATE UNIVERSITY	2121 EUCLID AVE	CLEVELAND	OH	44115	CUYAHOGA	USA	Biomedical Research and Research Training	000	4	10/16/2023	NON-COMPETING CONTINUATION	-$1,299
														Subtotal = -$1,961

Issue Date FY: 2021 ( Subtotal = $736,784 )
2021	2021	CLEVELAND STATE UNIVERSITY	2121 EUCLID AVE	CLEVELAND	OH	44115	CUYAHOGA	USA	Biomedical Research and Research Training	001	4	3/3/2021	NON-COMPETING CONTINUATION	$48,782
2021	2021	CLEVELAND STATE UNIVERSITY	2121 EUCLID AVE	CLEVELAND	OH	44115	CUYAHOGA	USA	Biomedical Research and Research Training	000	4	12/23/2020	NON-COMPETING CONTINUATION	$439,027
2021	2021	CLEVELAND STATE UNIVERSITY	2121 EUCLID AVE	CLEVELAND	OH	44115	CUYAHOGA	USA	Biomedical Research and Research Training	002	4	8/6/2021	SUPPLEMENT FOR EXPANSION	$248,975
														Subtotal = $736,784

Issue Date FY: 2020 ( Subtotal = $487,809 )
2020	2020	CLEVELAND STATE UNIVERSITY	2121 EUCLID AVE	CLEVELAND	OH	44115	CUYAHOGA	USA	Biomedical Research and Research Training	001	3	3/9/2020	NON-COMPETING CONTINUATION	$48,782
2020	2020	CLEVELAND STATE UNIVERSITY	2121 EUCLID AVE	CLEVELAND	OH	44115	CUYAHOGA	USA	Biomedical Research and Research Training	000	3	12/13/2019	NON-COMPETING CONTINUATION	$439,027
														Subtotal = $487,809

Issue Date FY: 2019 ( Subtotal = $487,809 )
2019	2019	CLEVELAND STATE UNIVERSITY	2121 EUCLID AVE	CLEVELAND	OH	44115	CUYAHOGA	USA	Biomedical Research and Research Training	000	2	12/12/2018	NON-COMPETING CONTINUATION	$487,809
														Subtotal = $487,809

Issue Date FY: 2018 ( Subtotal = $556,585 )
2018	2018	CLEVELAND STATE UNIVERSITY	2121 EUCLID AVE	CLEVELAND	OH	44115	CUYAHOGA	USA	Biomedical Research and Research Training	000	1	1/22/2018	NEW	$556,585
														Subtotal = $556,585

Grand Total All Awards = $2,267,026

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Replacement of Widefield Imaging System

Award Number: R01GM125800

ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 01/22/2018

PERIOD OF PERFORMANCE END DATE: 12/31/2022

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