Monday, June 30, 2025 6/30/2025

Genome-wide structural organization of proteins within human gene regulatory complexes

Award Number: R01GM125722
ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 01/19/2018
PERIOD OF PERFORMANCE END DATE: 12/31/2022

Group Awards By:

View Award Description

Genome-wide structural organization of proteins within human gene regulatory complexes - The DNA sequence of the human genome informs us as to the composition of proteins that make up healthy cells, but also altered compositions that create diseased cells. How protein production is controlled through the regulation of the genes that encode them is of critical importance for healthy and diseased cells. Knowing precisely where gene regulatory proteins bind, and are organized throughout the genome, including their interactions with each other, informs us as to how genes are regulated and mis-regulated. Since there are potentially thousands of different kinds of regulatory proteins and thousands of different kinds of human cell types and environmental responses that are a product of various subsets of regulatory proteins, the entire “universe” of gene regulatory events is quite substantial and consequently, quite costly to identify. A subset of these events will likely be informative or diagnostic of diseases states. Therefore, an important goal is to define informative interactions using cost-enabling, high accuracy, and robust genome-wide assays. To this end, ChIP-exo was developed to map the genomic binding locations of gene regulatory proteins at near-single base pair resolution. This assay will be applied, in high throughput, to determine the genome-wide positional organization of factors within protein-DNA complexes, like enhanceosomes. By broadly mapping the various classes of proteins that constitute much of the regulated epigenome, general rules about enhancer and repressor complex organization will be deduced. Aim 1 involves collecting genome-wide ChIP-exo data in human cell lines for a wide variety of protein-DNA complexes. Aim 2 will develop and implement computational approaches towards pattern recognition and data distillation in ChIP-exo datasets. The results are expected to provide structural insights into macromolecular protein complex assembly on a genomic scale, and in various cell types and conditions.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2024 ( Subtotal = -$3,800 )
2024	2021	THE PENNSYLVANIA STATE UNIVERSITY	201 OLD MAIN	UNIVERSITY PARK	PA	16802	CENTRE	USA	Biomedical Research and Research Training	000	4	10/17/2023	NON-COMPETING CONTINUATION	-$3,800
														Subtotal = -$3,800

Issue Date FY: 2021 ( Subtotal = $453,058 )
2021	2021	PENNSYLVANIA STATE UNIVERSITY, THE	201 OLD MAIN	UNIVERSITY PARK	PA	16802	CENTRE	USA	Biomedical Research and Research Training	000	4	12/23/2020	NON-COMPETING CONTINUATION	$407,752
2021	2021	PENNSYLVANIA STATE UNIVERSITY, THE	201 OLD MAIN	UNIVERSITY PARK	PA	16802	CENTRE	USA	Biomedical Research and Research Training	001	4	3/3/2021	NON-COMPETING CONTINUATION	$45,306
														Subtotal = $453,058

Issue Date FY: 2020 ( Subtotal = $386,674 )
2020	2020	PENNSYLVANIA STATE UNIVERSITY, THE	201 OLD MAIN	UNIVERSITY PARK	PA	16802	CENTRE	USA	Biomedical Research and Research Training	002	3	9/15/2020	SUPPLEMENT FOR EXPANSION	$66,712
2020	2020	PENNSYLVANIA STATE UNIVERSITY, THE	201 OLD MAIN	UNIVERSITY PARK	PA	16802	CENTRE	USA	Biomedical Research and Research Training	001	3	3/9/2020	NON-COMPETING CONTINUATION	$31,997
2020	2020	PENNSYLVANIA STATE UNIVERSITY, THE	201 OLD MAIN	UNIVERSITY PARK	PA	16802	CENTRE	USA	Biomedical Research and Research Training	000	3	12/30/2019	NON-COMPETING CONTINUATION	$287,965
														Subtotal = $386,674

Issue Date FY: 2019 ( Subtotal = $320,279 )
2019	2019	PENNSYLVANIA STATE UNIVERSITY, THE	201 OLD MAIN	UNIVERSITY PARK	PA	16802	CENTRE	USA	Biomedical Research and Research Training	000	2	12/18/2018	NON-COMPETING CONTINUATION	$320,279
														Subtotal = $320,279

Issue Date FY: 2018 ( Subtotal = $382,334 )
2018	2018	PENNSYLVANIA STATE UNIVERSITY, THE	201 OLD MAIN	UNIVERSITY PARK	PA	16802	CENTRE	USA	Biomedical Research and Research Training	001	1	9/17/2018	SUPPLEMENT FOR EXPANSION	$61,750
2018	2018	PENNSYLVANIA STATE UNIVERSITY, THE	201 OLD MAIN	UNIVERSITY PARK	PA	16802	CENTRE	USA	Biomedical Research and Research Training	000	1	1/18/2018	NEW	$320,584
														Subtotal = $382,334

Grand Total All Awards = $1,538,545

Top

All Categories

About

Search

Reports

Data Submission

Award Information

Genome-wide structural organization of proteins within human gene regulatory complexes

Award Number: R01GM125722

ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 01/19/2018

PERIOD OF PERFORMANCE END DATE: 12/31/2022

Federal Websites

Department of Health & Human Services

HHS Operating Divisions

HHS Staff Divisions

Download A Document Viewer