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Regulation of Planar Cell Polarity in Migrating Cells

Award Number: R01GM102356
ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 09/20/2013
PERIOD OF PERFORMANCE END DATE: 08/31/2020

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Regulation of Planar Cell Polarity in Migrating Cells - DESCRIPTION (provided by applicant): Planar cell polarity (PCP) describes the polarization of cells within the plane of a tissue and is an essential feature of embryonic morphogenesis. Disruption of vertebrate PCP is associated with multiple disorders including neural tube closure defects, ciliopathies, deafness, and potentially cancer. Two major questions face all fields of PCP research. First, what are the downstream effectors that transform signals from core PCP proteins into changes in cell polarity? Second, how is information communicated between cells to establish and maintain polarity in a planar tissue? Extensive characterization of PCP in the fly wing epithelium demonstrates those antagonistic interactions between Van Gogh and Frizzled (Fz)/Disheveled (Dsh) signaling function to polarize the formation of actin-rich hairs. By contrast despite a decade of speculation that asymmetric expression of these core PCP proteins polarizes the formation of membrane protrusions during gastrulation cell movements, the actual molecular mechanisms remain unclear. The goal of this proposal is to provide a new experimental paradigm explaining how PCP signaling coordinates the directed migration of polarized gastrula cell populations. Our central hypothesis is that Vang-like 2 (Vangl2) and Fz/Dsh signaling differentially impact cell-matrix interactions and cell-cell adhesion, respectivel, to control polarized membrane protrusive activity. Specific Aims: First, we will establish the mechanism whereby Vangl2 regulates Mmp14 activity, ECM proteolysis, and PCP. We will use a combination of in vivo molecular and genetic manipulations, cell transplantation methods, and confocal/time-lapse imaging to identify domains of Vangl2 expression and analyze membrane protrusion dynamics. We will use in vitro biochemical assays to identify signaling mechanisms connecting Vangl2 and integrin alpha5beta1 function. In the second aim, we will determine the contribution of cadherin-mediated adhesion to Fz/Dsh-dependent regulation of ECM assembly and PCP. We will use molecular and genetic methods, transplantations, and microscopic imaging to identify Fz, Dsh, and cadherin expression domains, manipulate cell adhesion, and analyze membrane protrusion dynamics. We will use biochemical methods to identify mechanisms whereby Fz/Dsh signaling regulates cell surface N-cadherin expression during gastrulation. In the third aim, we will dissect the abilities of Vangl2 and Fz/Dsh signaling to coordinate PCP across a field of migrating gastrula cells. Here, we will use a powerful combination of mosaic analyses and confocal imaging to quantify non-autonomous effects on membrane protrusion orientation, cell polarity, and PCP protein localization. Using these methods, we will identify both short- and long-range field effects and determine for the first time how changes in ECM structure and cell cohesion impact PCP during the collective movement of gastrula cells. Completion of this grant will significantly advance our understanding of vertebrate PCP. Since PCP occurs in the context of an ECM microenvironment and cell-cell adhesion, our data should have broad implications for other developmental and disease processes.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2022 ( Subtotal = -$9,079 )
2022	2017	MIDDLE TENNESSEE STATE UNIVERSITY	1301 E MAIN ST	MURFREESBORO	TN	37132	RUTHERFORD	USA	Biomedical Research and Research Training	000	5	12/2/2021	NON-COMPETING CONTINUATION	-$9,079
														Subtotal = -$9,079

Issue Date FY: 2019 ( Subtotal = $0 )
2019	2017	MIDDLE TENNESSEE STATE UNIVERSITY	1301 E MAIN ST	MURFREESBORO	TN	37132	RUTHERFORD	USA	Biomedical Research and Research Training	000	5	7/16/2019	NON-COMPETING CONTINUATION	$0
														Subtotal = $0

Issue Date FY: 2017 ( Subtotal = $247,000 )
2017	2017	MIDDLE TENNESSEE STATE UNIVERSITY	1301 E MAIN ST	MURFREESBORO	TN	37132	RUTHERFORD	USA	Biomedical Research and Research Training	001	5	8/22/2017	NON-COMPETING CONTINUATION	$247,000
2017	2015	MIDDLE TENNESSEE STATE UNIVERSITY	1301 E MAIN ST	MURFREESBORO	TN	37132	RUTHERFORD	USA	Biomedical Research and Research Training	000	3	8/16/2017	NON-COMPETING CONTINUATION	$0
														Subtotal = $247,000

Issue Date FY: 2016 ( Subtotal = $247,000 )
2016	2016	MIDDLE TENNESSEE STATE UNIVERSITY	1301 EAST MAIN STREET	MURFREESBORO	TN	37132	RUTHERFORD	USA	Biomedical Research and Research Training	000	4	8/15/2016	EXTENSION WITH OR WITHOUT FUNDS	$247,000
														Subtotal = $247,000

Issue Date FY: 2015 ( Subtotal = $247,000 )
2015	2015	MIDDLE TENNESSEE STATE UNIVERSITY	1301 EAST MAIN STREET	MURFREESBORO	TN	37132	RUTHERFORD	USA	Biomedical Research and Research Training	000	3	8/25/2015	NON-COMPETING CONTINUATION	$247,000
														Subtotal = $247,000

Issue Date FY: 2014 ( Subtotal = $247,000 )
2014	2014	MIDDLE TENNESSEE STATE UNIVERSITY	1301 EAST MAIN STREET	MURFREESBORO	TN	37132	RUTHERFORD	USA	Biomedical Research and Research Training	000	2	9/11/2014	NON-COMPETING CONTINUATION	$247,000
														Subtotal = $247,000

Issue Date FY: 2013 ( Subtotal = $242,896 )
2013	2013	MIDDLE TENNESSEE STATE UNIVERSITY	1301 EAST MAIN STREET	MURFREESBORO	TN	37132	RUTHERFORD	USA	Biomedical Research and Research Training	000	1	9/20/2013	NEW	$242,896
														Subtotal = $242,896

Grand Total All Awards = $1,221,817

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Regulation of Planar Cell Polarity in Migrating Cells

Award Number: R01GM102356

ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 09/20/2013

PERIOD OF PERFORMANCE END DATE: 08/31/2020

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