Thursday, September 18, 2025 9/18/2025

Phosphorylation and ubiquitination of immune sensory complexes in innate immune signaling

Award Number: R01GM097247
ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 07/01/2011
PERIOD OF PERFORMANCE END DATE: 11/30/2022

Group Awards By:

View Award Description

Phosphorylation and ubiquitination of immune sensory complexes in innate immune signaling - Phosphorylation and ubiquitination of immune sensory complexes in innate immune signaling Project Summary Plants and animals rely on pattern-recognition receptors (PRRs) to detect infection by recognizing microbe-associated molecular patterns (MAMPs). FLS2, a structural and functional ortholog of mammalian Toll-like receptors (TLRs) in plants, recognizes bacterial flagellin and initiates immune signaling by dimerizing with a co-receptor BAK1. With the previous NIH support, we have identified a plasma membrane-tethered receptor-like cytoplasmic kinase (RLCK) BIK1 as a convergent immune regulator by association with multiple PRR complexes. MAMP perception induces rapid BIK1 phosphorylation by BAK1, and subsequent release from PRR complexes to transduce diverse immune signaling. It remains unknown how PRR-associated BIK1 is activated and then bifurcates intracellular PRR signaling. Our preliminary data indicate that MAMP perception triggers rapid mono-ubiquitination of BIK1 and related RLCKs. A RING-type E3 ubiquitin ligase BMU1, that is phosphorylated by BIK1, mediates MAMP-induced BIK1 ubiquitination. BIK1 interacts and phosphorylates a diacylglycerol kinase DGK5 that regulates MAMP-induced phosphatidic acid (PA) production. Three over-arching aims are proposed to test a central hypothesis that mono-ubiquitination of BIK1 by BMU1 triggers release of BIK1 from PRR complexes, and subsequent endosomal trafficking and relaying intracellular signaling events, including activation of DGK5 for PA production. Three specific aims are 1. Ubiquitination of BIK1 by BIK1-phosphorylated BMU1 in plant immunity; 2. Ligand-induced BIK1 mono-ubiquitination in endosomal trafficking and plant immunity; 3. Dual phosphorylation of DGK5 by PRR-activated BIK1 and MAP kinase 4 in plant immunity. Recent advance on the molecular architecture of nonself recognition has revealed remarkable conservation in the mechanisms of microbial perception and innate immune signaling in multicellular eukaryotes. Phosphorylation and ubiquitination are two key mechanisms in regulating diverse cellular and organismal processes. Our project on pathogen- induced sequential and intertwined phosphorylation and ubiquitination orchestrating PRR complex activation and signal transduction in plant immunity will generate conceptual advance in understanding the biological functions of these two universal regulatory mechanisms at the whole organismal level. The proposed research will also contribute to the general understanding of innate immune signaling and immune sensory complex function.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2023 ( Subtotal = -$41 )
2023	2021	TEXAS A&M AGRILIFE RESEARCH	2147 TAMU	COLLEGE STATION	TX	77843	BRAZOS	USA	Biomedical Research and Research Training	000	9	4/28/2023	NON-COMPETING CONTINUATION	-$41
														Subtotal = -$41

Issue Date FY: 2021 ( Subtotal = $267,067 )
2021	2021	TEXAS A&M AGRILIFE RESEARCH	2147 TAMU	COLLEGE STATION	TX	77843	BRAZOS	USA	Biomedical Research and Research Training	000	9	11/25/2020	NON-COMPETING CONTINUATION	$240,360
2021	2021	TEXAS A&M AGRILIFE RESEARCH	2147 TAMU	COLLEGE STATION	TX	77843	BRAZOS	USA	Biomedical Research and Research Training	001	9	3/11/2021	NON-COMPETING CONTINUATION	$26,707
														Subtotal = $267,067

Issue Date FY: 2020 ( Subtotal = $439,302 )
2020	2020	TEXAS A&M AGRILIFE RESEARCH	2147 TAMU	COLLEGE STATION	TX	77843	BRAZOS	USA	Biomedical Research and Research Training	002	8	7/9/2020	SUPPLEMENT FOR EXPANSION	$171,870
2020	2020	TEXAS A&M AGRILIFE RESEARCH	2147 TAMU	COLLEGE STATION	TX	77843	BRAZOS	USA	Biomedical Research and Research Training	001	8	3/6/2020	NON-COMPETING CONTINUATION	$26,744
2020	2020	TEXAS A&M AGRILIFE RESEARCH	2147 TAMU	COLLEGE STATION	TX	77843	BRAZOS	USA	Biomedical Research and Research Training	000	8	12/5/2019	NON-COMPETING CONTINUATION	$240,688
														Subtotal = $439,302

Issue Date FY: 2019 ( Subtotal = $267,779 )
2019	2019	TEXAS A&M AGRILIFE RESEARCH	2147 TAMU	COLLEGE STATION	TX	77843	BRAZOS	USA	Biomedical Research and Research Training	000	7	12/28/2018	NON-COMPETING CONTINUATION	$267,779
														Subtotal = $267,779

Issue Date FY: 2018 ( Subtotal = $268,110 )
2018	2018	TEXAS A&M AGRILIFE RESEARCH	2147 TAMU	COLLEGE STATION	TX	77843	BRAZOS	USA	Biomedical Research and Research Training	000	6	12/2/2017	COMPETING CONTINUATION	$268,110
														Subtotal = $268,110

Issue Date FY: 2017 ( Subtotal = $0 )
2017	2015	TEXAS A & M RESEARCH FOUNDATION	400 HARVEY MITCHELL PKWY STE 300	COLLEGE STATION	TX	77845	BRAZOS	USA	Biomedical Research and Research Training	000	5	9/9/2017	NON-COMPETING CONTINUATION	$0
														Subtotal = $0

Issue Date FY: 2015 ( Subtotal = $243,249 )
2015	2015	TEXAS A & M RESEARCH FOUNDATION	400 Harvey Mitchell Parkway S,	COLLEGE STATION	TX	77845	BRAZOS	USA	Biomedical Research and Research Training	000	5	4/29/2015	NON-COMPETING CONTINUATION	$243,249
														Subtotal = $243,249

Issue Date FY: 2014 ( Subtotal = $243,249 )
2014	2014	TEXAS A & M RESEARCH FOUNDATION	400 Harvey Mitchell Parkway S,	COLLEGE STATION	TX	77845	BRAZOS	USA	Biomedical Research and Research Training	000	4	4/18/2014	NON-COMPETING CONTINUATION	$243,249
														Subtotal = $243,249

Issue Date FY: 2013 ( Subtotal = $234,735 )
2013	2013	TEXAS A & M RESEARCH FOUNDATION	400 Harvey Mitchell Parkway S,	COLLEGE STATION	TX	77845	BRAZOS	USA	Biomedical Research and Research Training	001	3	6/20/2013	NON-COMPETING CONTINUATION	$15,811
2013	2013	TEXAS A & M RESEARCH FOUNDATION	400 Harvey Mitchell Parkway S,	COLLEGE STATION	TX	77845	BRAZOS	USA	Biomedical Research and Research Training	000	3	4/4/2013	NON-COMPETING CONTINUATION	$218,924
														Subtotal = $234,735

Issue Date FY: 2012 ( Subtotal = $243,249 )
2012	2012	TEXAS A & M RESEARCH FOUNDATION	400 Harvey Mitchell Parkway S,	COLLEGE STATION	TX	77845	BRAZOS	USA	Biomedical Research and Research Training	000	2	4/27/2012	NON-COMPETING CONTINUATION	$243,249
														Subtotal = $243,249

Issue Date FY: 2011 ( Subtotal = $243,249 )
2011	2011	TEXAS A & M RESEARCH FOUNDATION	400 Harvey Mitchell Parkway S,	COLLEGE STATION	TX	77845	BRAZOS	USA	Biomedical Research and Research Training	000	1	6/28/2011	NEW	$243,249
														Subtotal = $243,249

Grand Total All Awards = $2,449,948

Top

All Categories

About

Search

Reports

Data Submission

Award Information

Phosphorylation and ubiquitination of immune sensory complexes in innate immune signaling

Award Number: R01GM097247

ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 07/01/2011

PERIOD OF PERFORMANCE END DATE: 11/30/2022

Federal Websites

Department of Health & Human Services

HHS Operating Divisions

HHS Staff Divisions

Download A Document Viewer