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RECOGNITION AND PHAGOCYTOSIS OF BACTERIA IN DROSOPHILA

Award Number: R01GM073701
ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 02/01/2007
PERIOD OF PERFORMANCE END DATE: 03/27/2010

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RECOGNITION AND PHAGOCYTOSIS OF BACTERIA IN DROSOPHILA - DESCRIPTION (provided by applicant): In innate immunity, pathogen associated molecular patterns (PAMPs) are recognized by pattern recognition receptors (PRRs) that activate signaling cascades and effector mechanisms, like phagocytosis which act to clear the microbes out of the host's system. We are interested in the phagocytosis of bacteria in Drosophila because not much is know about cellular immunity in flies and it may be the first-line immune response. In a pilot genetic screen, we have identified 8 mutations that affect the phagocytosis of E. coli (a Gram- bacteria) and/or S. aureus (a Gram+ bacteria) in vivo. The specific aims of this proposal are: 1. To elucidate the role two catalytic peptidoglycan recognition proteins (PGRP-SC1a/b and PGRP-SC2) play in the phagocytosis of bacteria and the activation of the antimicrobial peptide (AMP) response. Two of our phagocytosis mutations affect PGRP-SC1a/b and PGRP-SC2 expression. We will examine how these mutants affect phagocytosis and AMP responses. 2. To determine if disruption of the outer membrane of Gram-negative bacteria is necessary for recognition and clearance of the bacteria by the Drosophila immune response. We believe that this may be occuring to allow access to peptidoglycan in the cell wall which can trigger the downstream AMP responses. 3. To examine how genes important for immune responses or phagocytosis affect phagosome maturation in vivo. We have preliminary data indicating that PGRP-SC2, Rab14 and AMPs may be important for phagosome maturation. With these experiments, we hope to understand the role these genes play in recognition and phagocytosis of bacteria in vivo.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2010 ( Subtotal = $148 )
2010	2009	MARYLAND BIOTECHNOLOGY INSTITUTE	701 E PRATT STREET, SUITE 200	BALTIMORE	MD	21202	BALTIMORE CITY	USA	Biomedical Research and Research Training	002	3	5/10/2010	SUPPLEMENT FOR EXPANSION	-$9,306
2010	2009	MARYLAND BIOTECHNOLOGY INSTITUTE	701 E PRATT STREET, SUITE 200	BALTIMORE	MD	21202	BALTIMORE CITY	USA	Biomedical Research and Research Training	000	3	5/10/2010	NON-COMPETING CONTINUATION	-$14,611
2010	2009	UNIVERSITY OF MARYLAND	4101 CHESAPEAKE BLDG	COLLEGE PARK	MD	20742	PRINCE GEORGES	USA	Biomedical Research and Research Training	001	4	5/10/2010	CHANGE OF GRANTEE / TRAINING INSTITUTION / AWARDING INSTITUTION	$24,065
														Subtotal = $148

Issue Date FY: 2009 ( Subtotal = $308,861 )
2009	2009	MARYLAND BIOTECHNOLOGY INSTITUTE	701 E PRATT STREET, SUITE 200	BALTIMORE	MD	21202	BALTIMORE CITY	USA	Biomedical Research and Research Training	002	3	4/20/2009	SUPPLEMENT FOR EXPANSION	$16,524
2009	2009	MARYLAND BIOTECHNOLOGY INSTITUTE	701 E PRATT STREET, SUITE 200	BALTIMORE	MD	21202	BALTIMORE CITY	USA	Biomedical Research and Research Training	001	3	5/7/2009	NON-COMPETING CONTINUATION	$29,234
2009	2009	MARYLAND BIOTECHNOLOGY INSTITUTE	701 E PRATT STREET, SUITE 200	BALTIMORE	MD	21202	BALTIMORE CITY	USA	Biomedical Research and Research Training	000	3	1/29/2009	NON-COMPETING CONTINUATION	$263,103
														Subtotal = $308,861

Issue Date FY: 2008 ( Subtotal = $292,517 )
2008	2008	MARYLAND BIOTECHNOLOGY INSTITUTE	701 E PRATT STREET, SUITE 200	BALTIMORE	MD	21202	BALTIMORE CITY	USA	Biomedical Research and Research Training	000	2	2/22/2008	NON-COMPETING CONTINUATION	$292,517
														Subtotal = $292,517

Issue Date FY: 2007 ( Subtotal = $281,480 )
2007	2007	MARYLAND BIOTECHNOLOGY INSTITUTE	701 E PRATT STREET, SUITE 200	BALTIMORE	MD	21202	BALTIMORE CITY	USA	Biomedical Research and Research Training	000	1	1/8/2007	NEW	$281,480
														Subtotal = $281,480

Grand Total All Awards = $883,006

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RECOGNITION AND PHAGOCYTOSIS OF BACTERIA IN DROSOPHILA

Award Number: R01GM073701

ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 02/01/2007

PERIOD OF PERFORMANCE END DATE: 03/27/2010

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