Official websites use .gov

A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS

A lock ( 🔒 ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

Need Help

Bioengineering approaches to map mechanotransduction in the living cell

Award Number: R01GM072744
ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 08/01/2005
PERIOD OF PERFORMANCE END DATE: 05/31/2027

Group Awards By:

View Award Description

Bioengineering approaches to map mechanotransduction in the living cell - Project Summary/Abstract It is well known that stem cell differentiation and cell fate are regulated by mechanical forces and mechanics. We have reported earlier that cell mechanical properties dictate stress-induced spreading and differentiation in embryonic stem cells (ESCs). Other reports have also demonstrated the effect of forces on pluripotent stem cells. However, how force regulates (pluripotent) stem cell differentiation and cell fate at the level of chromatin remains elusive. This is an important question since the chromatin is the hub of gene transcription and DNA replication and DNA damage repair, critical for pluripotent stem cell self-renewal and differentiation and cancer stem cell self-renewal. Our preliminary results chromatin domain stretching but not compression rapidly and nuclear protein LAP2β mediates force transmission from nuclear lamina to chromatin. In addition, RNA polymerase II (Pol II) is recruited and elongated in response to stretching but not compression and demethylation of H3K9me3 is necessary for force-induced gene upregulation. Built on these results, we propose 3 specific aims to elucidate nuclear mechanobiology mechanisms in the living cells. Aim 1: To determine if chromatin stretching is necessary for force-induced stem cell differentiation; Aim 2: To test the hypothesis that H3K9me3 and H3K9ac regulate force-induced pluripotent stem cell gene transcription; Aim 3: To determine if large-strain induced telomere attrition contributes to transition from normal stem cells to cancer stem cells. Our experimental designs are rigorous and the likelihood of generating insightful discovery is high. The long-term goal is to develop novel approaches and strategies to intervene pathological processes like malignant tumors.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2026 ( Subtotal = $400,000 )
2026	2026	NORTHEASTERN UNIVERSITY	360 HUNTINGTON AVE	BOSTON	MA	02115	SUFFOLK	USA	Biomedical Research and Research Training	000	22	5/7/2026	NON-COMPETING CONTINUATION	$400,000
														Subtotal = $400,000

Issue Date FY: 2025 ( Subtotal = $400,000 )
2025	2025	NORTHEASTERN UNIVERSITY	360 HUNTINGTON AVE	BOSTON	MA	02115	SUFFOLK	USA	Biomedical Research and Research Training	000	21	8/7/2025	NON-COMPETING CONTINUATION	$400,000
														Subtotal = $400,000

Issue Date FY: 2024 ( Subtotal = $399,896 )
2024	2024	NORTHEASTERN UNIVERSITY	360 HUNTINGTON AVE	BOSTON	MA	02115	SUFFOLK	USA	Biomedical Research and Research Training	001	20	5/15/2024	NON-COMPETING CONTINUATION	$399,896
2024	2022	UNIVERSITY OF ILLINOIS	506 S WRIGHT ST	URBANA	IL	61801	CHAMPAIGN	USA	Biomedical Research and Research Training	000	18	4/22/2024	NON-COMPETING CONTINUATION	$0
														Subtotal = $399,896

Issue Date FY: 2023 ( Subtotal = $398,750 )
2023	2023	NORTHEASTERN UNIVERSITY	360 HUNTINGTON AVE	BOSTON	MA	02115	SUFFOLK	USA	Biomedical Research and Research Training	000	19	8/24/2023	COMPETING CONTINUATION	$398,750
														Subtotal = $398,750

Issue Date FY: 2022 ( Subtotal = $389,025 )
2022	2022	UNIVERSITY OF ILLINOIS	352 HENRY ADMINISTRATION BLDG	URBANA	IL	61801	CHAMPAIGN	USA	Biomedical Research and Research Training	000	18	2/15/2022	NON-COMPETING CONTINUATION	$350,122
2022	2022	UNIVERSITY OF ILLINOIS	506 S WRIGHT ST	URBANA	IL	61801	CHAMPAIGN	USA	Biomedical Research and Research Training	001	18	6/27/2022	NON-COMPETING CONTINUATION	$38,903
														Subtotal = $389,025

Issue Date FY: 2021 ( Subtotal = $389,242 )
2021	2021	UNIVERSITY OF ILLINOIS	364 HENRY ADMINISTRATION BLDG	URBANA	IL	61801	CHAMPAIGN	USA	Biomedical Research and Research Training	000	17	2/23/2021	NON-COMPETING CONTINUATION	$389,242
														Subtotal = $389,242

Issue Date FY: 2020 ( Subtotal = $389,454 )
2020	2020	UNIVERSITY OF ILLINOIS	364 HENRY ADMINISTRATION BLDG	URBANA	IL	61801	CHAMPAIGN	USA	Biomedical Research and Research Training	001	16	3/12/2020	NON-COMPETING CONTINUATION	$389,454
2020	2018	UNIVERSITY OF ILLINOIS	364 HENRY ADMINISTRATION BLDG	URBANA	IL	61801	CHAMPAIGN	USA	Biomedical Research and Research Training	000	14	2/19/2020	NON-COMPETING CONTINUATION	$0
														Subtotal = $389,454

Issue Date FY: 2019 ( Subtotal = $389,659 )
2019	2019	UNIVERSITY OF ILLINOIS	364 HENRY ADMINISTRATION BLDG	URBANA	IL	61801	CHAMPAIGN	USA	Biomedical Research and Research Training	001	15	5/14/2019	COMPETING CONTINUATION	$389,659
2019	2018	UNIVERSITY OF ILLINOIS	364 HENRY ADMINISTRATION BLDG	URBANA	IL	61801	CHAMPAIGN	USA	Biomedical Research and Research Training	000	14	5/9/2019	NON-COMPETING CONTINUATION	$0
														Subtotal = $389,659

Issue Date FY: 2018 ( Subtotal = $370,714 )
2018	2018	UNIVERSITY OF ILLINOIS	506 S WRIGHT ST STE 364	URBANA	IL	61801	CHAMPAIGN	USA	Biomedical Research and Research Training	000	14	1/24/2018	NON-COMPETING CONTINUATION	$333,642
2018	2018	UNIVERSITY OF ILLINOIS	506 S WRIGHT ST STE 364	URBANA	IL	61801	CHAMPAIGN	USA	Biomedical Research and Research Training	001	14	5/7/2018	NON-COMPETING CONTINUATION	$37,072
														Subtotal = $370,714

Issue Date FY: 2017 ( Subtotal = $371,065 )
2017	2017	UNIVERSITY OF ILLINOIS	506 S WRIGHT ST STE 364	URBANA	IL	61801	CHAMPAIGN	USA	Biomedical Research and Research Training	001	13	8/15/2017	NON-COMPETING CONTINUATION	$37,107
2017	2017	UNIVERSITY OF ILLINOIS	506 S WRIGHT ST STE 364	URBANA	IL	61801	CHAMPAIGN	USA	Biomedical Research and Research Training	000	13	11/8/2016	NON-COMPETING CONTINUATION	$333,958
														Subtotal = $371,065

Issue Date FY: 2016 ( Subtotal = $371,405 )
2016	2016	UNIVERSITY OF ILLINOIS BOARD OF TRUSTEES	109 COBLE HALL, 801 S WRIGHT S	CHAMPAIGN	IL	61820	CHAMPAIGN	USA	Biomedical Research and Research Training	001	12	4/6/2016	NON-COMPETING CONTINUATION	$37,141
2016	2016	UNIVERSITY OF ILLINOIS BOARD OF TRUSTEES	109 COBLE HALL, 801 S WRIGHT S	CHAMPAIGN	IL	61820	CHAMPAIGN	USA	Biomedical Research and Research Training	000	12	11/18/2015	NON-COMPETING CONTINUATION	$334,264
														Subtotal = $371,405

Issue Date FY: 2015 ( Subtotal = $371,733 )
2015	2015	UNIVERSITY OF ILLINOIS BOARD OF TRUSTEES	109 COBLE HALL, 801 S WRIGHT S	CHAMPAIGN	IL	61820	CHAMPAIGN	USA	Biomedical Research and Research Training	002	11	12/18/2014	COMPETING CONTINUATION	$371,733
2015	2014	UNIVERSITY OF ILLINOIS BOARD OF TRUSTEES	109 COBLE HALL, 801 S WRIGHT S	CHAMPAIGN	IL	61820	CHAMPAIGN	USA	Biomedical Research and Research Training	001	10	12/15/2014	SUPPLEMENT FOR EXPANSION	$0
2015	2013	UNIVERSITY OF ILLINOIS BOARD OF TRUSTEES	109 COBLE HALL, 801 S WRIGHT S	CHAMPAIGN	IL	61820	CHAMPAIGN	USA	Biomedical Research and Research Training	000	10	12/15/2014	NON-COMPETING CONTINUATION	$0
														Subtotal = $371,733

Issue Date FY: 2014 ( Subtotal = $118,342 )
2014	2014	UNIVERSITY OF ILLINOIS BOARD OF TRUSTEES	109 COBLE HALL, 801 S WRIGHT S	CHAMPAIGN	IL	61820	CHAMPAIGN	USA	Biomedical Research and Research Training	000	10	6/16/2014	SUPPLEMENT FOR EXPANSION	$118,342
2014	2013	UNIVERSITY OF ILLINOIS BOARD OF TRUSTEES	109 COBLE HALL, 801 S WRIGHT S	CHAMPAIGN	IL	61820	CHAMPAIGN	USA	Biomedical Research and Research Training	001	10	6/16/2014	NON-COMPETING CONTINUATION	$0
														Subtotal = $118,342

Issue Date FY: 2013 ( Subtotal = $354,813 )
2013	2013	UNIVERSITY OF ILLINOIS BOARD OF TRUSTEES	109 COBLE HALL, 801 S WRIGHT S	CHAMPAIGN	IL	61820	CHAMPAIGN	USA	Biomedical Research and Research Training	000	10	8/26/2013	NON-COMPETING CONTINUATION	$354,813
														Subtotal = $354,813

Issue Date FY: 2012 ( Subtotal = $368,026 )
2012	2012	UNIVERSITY OF ILLINOIS BOARD OF TRUSTEES	109 COBLE HALL, 801 S WRIGHT S	CHAMPAIGN	IL	61820	CHAMPAIGN	USA	Biomedical Research and Research Training	000	9	8/1/2012	NON-COMPETING CONTINUATION	$368,026
														Subtotal = $368,026

Issue Date FY: 2011 ( Subtotal = $368,354 )
2011	2011	UNIVERSITY OF ILLINOIS BOARD OF TRUSTEES	109 COBLE HALL, 801 S WRIGHT S	CHAMPAIGN	IL	61820	CHAMPAIGN	USA	Biomedical Research and Research Training	000	8	8/5/2011	NON-COMPETING CONTINUATION	$368,354
														Subtotal = $368,354

Issue Date FY: 2010 ( Subtotal = $372,395 )
2010	2010	UNIVERSITY OF ILLINOIS BOARD OF TRUSTEES	109 COBLE HALL, 801 S WRIGHT S	CHAMPAIGN	IL	61820	CHAMPAIGN	USA	Biomedical Research and Research Training	000	7	6/21/2010	COMPETING CONTINUATION	$372,395
														Subtotal = $372,395

Issue Date FY: 2009 ( Subtotal = $380,434 )
2009	2009	UNIVERSITY OF ILLINOIS BOARD OF TRUSTEES	109 COBLE HALL, 801 S WRIGHT S	CHAMPAIGN	IL	61820	CHAMPAIGN	USA	Biomedical Research and Research Training	001	6	8/26/2009	NON-COMPETING CONTINUATION	$314,616
2009	2009	UNIVERSITY OF ILLINOIS BOARD OF TRUSTEES	109 COBLE HALL, 801 S WRIGHT S	CHAMPAIGN	IL	61820	CHAMPAIGN	USA	Biomedical Research and Research Training	000	5	3/6/2009	SUPPLEMENT FOR EXPANSION	$65,818
														Subtotal = $380,434

Issue Date FY: 2008 ( Subtotal = $248,994 )
2008	2008	UNIVERSITY OF ILLINOIS BOARD OF TRUSTEES	109 COBLE HALL, 801 S WRIGHT S	CHAMPAIGN	IL	61820	CHAMPAIGN	USA	Biomedical Research and Research Training	000	5	7/21/2008	NON-COMPETING CONTINUATION	$248,994
														Subtotal = $248,994

Issue Date FY: 2007 ( Subtotal = $249,182 ) (Continued on the next page)
Grand Total All Awards = $7,645,293

Page 1 of 2 (55 items)12

Top

All Categories

About

Search

Reports

Data Submission

Award Information

Bioengineering approaches to map mechanotransduction in the living cell

Award Number: R01GM072744

ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 08/01/2005

PERIOD OF PERFORMANCE END DATE: 05/31/2027

Federal Websites

Department of Health & Human Services

HHS Operating Divisions

HHS Staff Divisions

Download A Document Viewer