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Cytokinesis and RNA segregation in zebrafish development

Award Number: R01GM065303
ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 03/01/2002
PERIOD OF PERFORMANCE END DATE: 02/29/2024

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Cytokinesis and RNA segregation in zebrafish development - Project Summary / Abstract In many animal species, germ cell fate determination depends on the maternal inheritance of the germ plasm, a specialized cytoplasm composed of ribonucleoparticles (RNPs) and enriched in cytoskeletal elements. Germ plasm RNPs are typically inherited as particles within the egg, which during early embryogenesis become aggregated into large masses. These become segregated into primordial germ cells (PGCs), where they are thought to promote the initiation of the germ cell transcriptional program. In zebrafish, as in a number of other vertebrate species, gathering and segregation of germ plasm RNPs is coupled to cell division. Zebrafish germ plasm has been shown to be required and sufficient for germ cell induction in this organism. We have previously delineated specific steps during cell division that result in germ plasm RNP recruitment to the furrows for the first several cycles, with subsequent compaction at the furrow distal ends. These steps entail a gradual increase in germ plasm RNP aggregation and result in the stabilization of four large germ plasm masses. These masses are inherited by PGCs, to eventually disperse through the cytoplasm during PGC specification. Our overarching hypothesis is that germ plasm RNP aggregation is mediated by adaptations of the cellular machinery, relying on the interaction between a dynamic, excitable cortex and fluid phase-like properties of RNPs and associated intrinsically disordered proteins (IDPs). In Aim 1, we will address how the regulation of an excitable actomyosin network mediates germ plasm RNP movement and aggregation, and will study the function of factors components of the Chromosomal Passenger Complex (CPC) in cytoskeletal network dynamics and germ plasm RNP anchoring. In Aim 2, we will study the behavior of segregating germ plasm RNPs as ordered supramolecular structures at the furrows, as well as the role in germ plasm segregation of the previously uncharacterized IDP Bucky ball 2-like. The germ cell fate shares characteristics with pluripotency and cancer, and our studies will provide new knowledge applicable not only to reproduction but also cell reprogramming and cancer biology. Understanding of membrane-less compartmentalization through fluid phase behavior will provide additional fundamental insights relevant to human biology and disease.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2024 ( Subtotal = $0 )
2024	2023	UNIVERSITY OF WISCONSIN SYSTEM	21 N PARK ST STE 6301	MADISON	WI	53715	DANE	USA	Biomedical Research and Research Training	000	17	8/27/2024	NON-COMPETING CONTINUATION	$0
														Subtotal = $0

Issue Date FY: 2023 ( Subtotal = $306,352 )
2023	2023	UNIVERSITY OF WISCONSIN SYSTEM	21 N PARK ST STE 6301	MADISON	WI	53715	DANE	USA	Biomedical Research and Research Training	000	17	2/16/2023	NON-COMPETING CONTINUATION	$306,352
														Subtotal = $306,352

Issue Date FY: 2022 ( Subtotal = $306,352 )
2022	2022	UNIVERSITY OF WISCONSIN SYSTEM	21 N PARK ST STE 6301	MADISON	WI	53715	DANE	USA	Biomedical Research and Research Training	001	16	7/27/2022	NON-COMPETING CONTINUATION	$30,636
2022	2022	UNIVERSITY OF WISCONSIN SYSTEM	21 N PARK ST STE 6301	MADISON	WI	53715	DANE	USA	Biomedical Research and Research Training	000	16	2/21/2022	NON-COMPETING CONTINUATION	$275,716
														Subtotal = $306,352

Issue Date FY: 2021 ( Subtotal = $306,034 )
2021	2021	UNIVERSITY OF WISCONSIN SYSTEM	21 N PARK ST STE 6401	MADISON	WI	53715	DANE	USA	Biomedical Research and Research Training	000	15	3/25/2021	NON-COMPETING CONTINUATION	$306,034
														Subtotal = $306,034

Issue Date FY: 2020 ( Subtotal = $311,248 )
2020	2020	UNIVERSITY OF WISCONSIN SYSTEM	21 N PARK ST STE 6401	MADISON	WI	53715	DANE	USA	Biomedical Research and Research Training	000	14	2/28/2020	COMPETING CONTINUATION	$305,398
2020	2020	UNIVERSITY OF WISCONSIN SYSTEM	21 N PARK ST STE 6401	MADISON	WI	53715	DANE	USA	Biomedical Research and Research Training	001	14	3/6/2020	COMPETING CONTINUATION	$5,850
														Subtotal = $311,248

Issue Date FY: 2019 ( Subtotal = -$5,850 )
2019	2017	UNIVERSITY OF WISCONSIN SYSTEM	21 N PARK ST STE 6401	MADISON	WI	53715	DANE	USA	Biomedical Research and Research Training	000	13	5/20/2019	NON-COMPETING CONTINUATION	-$5,850
														Subtotal = -$5,850

Issue Date FY: 2017 ( Subtotal = $286,953 )
2017	2017	UNIVERSITY OF WISCONSIN SYSTEM	21 N PARK ST STE 6401	MADISON	WI	53715	DANE	USA	Biomedical Research and Research Training	000	13	1/26/2017	NON-COMPETING CONTINUATION	$258,257
2017	2017	UNIVERSITY OF WISCONSIN SYSTEM	21 N PARK ST STE 6401	MADISON	WI	53715	DANE	USA	Biomedical Research and Research Training	001	13	6/20/2017	NON-COMPETING CONTINUATION	$28,696
														Subtotal = $286,953

Issue Date FY: 2016 ( Subtotal = $286,953 )
2016	2016	UNIVERSITY OF WISCONSIN SYSTEM/BOARD OF REGENTS	21 N PARK ST	MADISON	WI	53715	DANE	USA	Biomedical Research and Research Training	000	12	3/8/2016	NON-COMPETING CONTINUATION	$286,953
														Subtotal = $286,953

Issue Date FY: 2015 ( Subtotal = $332,601 )
2015	2015	UNIVERSITY OF WISCONSIN SYSTEM/BOARD OF REGENTS	21 N PARK ST	MADISON	WI	53715	DANE	USA	Biomedical Research and Research Training	000	11	4/22/2015	NON-COMPETING CONTINUATION	$332,601
														Subtotal = $332,601

Issue Date FY: 2014 ( Subtotal = $302,168 )
2014	2014	UNIVERSITY OF WISCONSIN SYSTEM/BOARD OF REGENTS	21 N PARK ST	MADISON	WI	53715	DANE	USA	Biomedical Research and Research Training	001	10	9/15/2014	SUPPLEMENT FOR EXPANSION	$15,215
2014	2014	UNIVERSITY OF WISCONSIN SYSTEM/BOARD OF REGENTS	21 N PARK ST	MADISON	WI	53715	DANE	USA	Biomedical Research and Research Training	000	10	12/26/2013	COMPETING CONTINUATION	$286,953
														Subtotal = $302,168

Issue Date FY: 2011 ( Subtotal = $272,922 )
2011	2011	UNIVERSITY OF WISCONSIN SYSTEM/BOARD OF REGENTS	21 N PARK ST	MADISON	WI	53715	DANE	USA	Biomedical Research and Research Training	000	9	6/13/2011	NON-COMPETING CONTINUATION	$272,922
														Subtotal = $272,922

Issue Date FY: 2010 ( Subtotal = $275,679 )
2010	2010	UNIVERSITY OF WISCONSIN SYSTEM/BOARD OF REGENTS	21 N PARK ST	MADISON	WI	53715	DANE	USA	Biomedical Research and Research Training	000	8	6/28/2010	NON-COMPETING CONTINUATION	$275,679
														Subtotal = $275,679

Issue Date FY: 2009 ( Subtotal = $278,464 )
2009	2009	UNIVERSITY OF WISCONSIN SYSTEM/BOARD OF REGENTS	21 N PARK ST	MADISON	WI	53715	DANE	USA	Biomedical Research and Research Training	000	7	6/9/2009	NON-COMPETING CONTINUATION	$278,464
														Subtotal = $278,464

Issue Date FY: 2008 ( Subtotal = $278,464 )
2008	2008	UNIVERSITY OF WISCONSIN SYSTEM/BOARD OF REGENTS	21 NORTH PARK STREET	MADISON	WI	53715	DANE	USA	Biomedical Research and Research Training	000	6	6/13/2008	COMPETING CONTINUATION	$278,464
														Subtotal = $278,464

Issue Date FY: 2006 ( Subtotal = $239,036 )
2006	2006	UNIVERSITY OF WISCONSIN SYSTEM/BOARD OF REGENTS	750 UNIVERSITY AVENUE	MADISON	WI	53706	DANE	USA	Biomedical Research and Research Training	000	5	2/20/2006	NON-COMPETING CONTINUATION	$239,036
														Subtotal = $239,036

Issue Date FY: 2005 ( Subtotal = $244,864 )
2005	2005	UNIVERSITY OF WISCONSIN SYSTEM/BOARD OF REGENTS	750 UNIVERSITY AVENUE	MADISON	WI	53706	DANE	USA	Biomedical Research and Research Training	000	4	2/18/2005	NON-COMPETING CONTINUATION	$244,864
														Subtotal = $244,864

Issue Date FY: 2004 ( Subtotal = $244,936 )
2004	2004	UNIVERSITY OF WISCONSIN SYSTEM/BOARD OF REGENTS	750 UNIVERSITY AVENUE	MADISON	WI	53706	DANE	USA	Biomedical Research and Research Training	000	3	1/12/2004	NON-COMPETING CONTINUATION	$244,936
														Subtotal = $244,936

Issue Date FY: 2003 ( Subtotal = $245,007 )
2003	2003	UNIVERSITY OF WISCONSIN SYSTEM/BOARD OF REGENTS	750 UNIVERSITY AVENUE	MADISON	WI	53706	DANE	USA	Genetics and Developmental Biology Research and Research Training	000	2		NON-COMPETING CONTINUATION	$245,007
														Subtotal = $245,007

Issue Date FY: 2002 ( Subtotal = $216,146 )
2002	2002	UNIVERSITY OF WISCONSIN SYSTEM/BOARD OF REGENTS	750 UNIVERSITY AVENUE	MADISON	WI	53706	DANE	USA	Genetics and Developmental Biology Research and Research Training	000	1	2/15/2002	NEW	$216,146
														Subtotal = $216,146

Grand Total All Awards = $4,728,329

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Cytokinesis and RNA segregation in zebrafish development

Award Number: R01GM065303

ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 03/01/2002

PERIOD OF PERFORMANCE END DATE: 02/29/2024

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