Monday, February 16, 2026 2/16/2026

REACTIONS OF N-NITROSO COMPOUNDS WITH HEME

Award Number: R01GM064476
ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 08/01/2002
PERIOD OF PERFORMANCE END DATE: 05/02/2007

Group Awards By:

View Award Description

REACTIONS OF N-NITROSO COMPOUNDS WITH HEME - DESCRIPTION (provided by applicant): Nitric oxide (NO) is now one of the most studied small molecules in bioinorganic chemistry, and new discoveries of the consequence of NO binding to the metal center in heme continue to be made. N -nitroso compounds are also very important in the field of nitric oxide chemistry. Not only can they serve as sources of NO for metal-NO bond formation, but they are also associated with incidences of cancer after biochemical activation of nitrosmines and related compounds by the heme-containing cytochrome P450 enzyme. The iron center in cytochrome P450 has been implicated in some metabolic reactions of N -nitroso compounds. Surprisingly, however, metalloporphyrin-nitrosamine chemistry is an unexplored area of research. The biological and environmental occurrence of N -nitroso compounds, and the fact that they are metabolized by the heme-containing cytochrome P450 enzymes, underscores the necessity to examine their (N -nitroso) interactions with heme models and with heme-containing proteins as a means of better understanding their fundamental chemistry.In this proposal, we hypothesize that the bio-related chemistry of various organic N -nitroso compounds is interwoven with the chemistry of NO, and that heme iron is an important contributor in the biological NO-to-XNO conversion. Thus, a detailed knowledge of iron porphyrin-nitrosamine chemistry and subsequent Fe-NO bond formation will provide the necessary information needed to help elucidate some of the important metabolic pathways that involve nitrosamine interactions with heme biomolecules such as cytochrome P450.We have previously reported the preparation and reactions of a number of nitrosyl and nitroso (nitrosamine, nitrosoarene, nitrosothiol, etc.) complexes of metalloporphyrins. We are now entering the second major phase of our study to develop the representative reaction chemistry of these nitrosyl and nitroso metalloporphyrins. We propose to examine the interactions of nitrosamines and related compounds with heme models and with cytochrome P450 BM3 (both the heme domain and the full-length protein), and elucidate the reaction pathways of these heme-nitrosamine complexes. This planned research is fundamental to the understanding of the heme-dependent bloinorganic chemistry of NO.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2007 ( Subtotal = $83,250 )
2007	2007	UNIVERSITY OF OKLAHOMA NORMAN CAMPUS	1000 ASP AVENUE	NORMAN	OK	73109		USA	Biomedical Research and Research Training	001	4	12/25/2006	SUPPLEMENT FOR EXPANSION	$83,250
														Subtotal = $83,250

Issue Date FY: 2005 ( Subtotal = $246,650 )
2005	2005	UNIVERSITY OF OKLAHOMA NORMAN CAMPUS	1000 ASP AVENUE	NORMAN	OK	73109		USA	Biomedical Research and Research Training	000	4	8/4/2005	NON-COMPETING CONTINUATION	$246,650
														Subtotal = $246,650

Issue Date FY: 2004 ( Subtotal = $261,900 )
2004	2004	UNIVERSITY OF OKLAHOMA NORMAN CAMPUS	555 CONSTITUTION ST., STE. 237	NORMAN	OK	73072	CLEVELAND	USA	Biomedical Research and Research Training	000	3	7/26/2004	NON-COMPETING CONTINUATION	$261,900
														Subtotal = $261,900

Issue Date FY: 2003 ( Subtotal = $261,900 )
2003	2003	UNIVERSITY OF OKLAHOMA NORMAN CAMPUS	555 CONSTITUTION ST., STE. 237	NORMAN	OK	73072	CLEVELAND	USA	Biomedical Research and Research Training	000	2	7/25/2003	NON-COMPETING CONTINUATION	$261,900
														Subtotal = $261,900

Issue Date FY: 2002 ( Subtotal = $320,256 )
2002	2002	UNIVERSITY OF OKLAHOMA NORMAN CAMPUS	555 CONSTITUTION ST., STE. 237	NORMAN	OK	73072	CLEVELAND	USA	Biomedical Research and Research Training	000	1	7/5/2002	NEW	$320,256
														Subtotal = $320,256

Grand Total All Awards = $1,173,956

Top

All Categories

About

Search

Reports

Data Submission

Award Information

REACTIONS OF N-NITROSO COMPOUNDS WITH HEME

Award Number: R01GM064476

ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 08/01/2002

PERIOD OF PERFORMANCE END DATE: 05/02/2007

Federal Websites

Department of Health & Human Services

HHS Operating Divisions

HHS Staff Divisions

Download A Document Viewer