Friday, May 3, 2024 5/3/2024

Chromatin Domain Structure and Function

Award Number: R01GM058460
ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

Group Awards By Issue Date FY or Funding FY:

View Award Abstract

Increasing genomic and cytological evidence suggests that where a gene is located within the nucleus and how it is folded within the interphase chromosome may produce comparable increases in a gene's transcriptional output as the initial binding of transcription factors to promoter sequences. Our long-term goals are to determine the intranuclear positioning and large-scale chromatin structure of specific gene loci, to identify the cis and trans determinants of this positioning and folding, and to determine the functional significance of this level of chromatin organization. Our hypothesis is that gene positioning and large-scale chromatin folding create a global chromatin environment whose influence on transcriptional regulation of certain genes is quantitatively is at least as large as the influence of local cis regulatory elements. Our goals for the next funding period will be to: 1) develop a new genomic method for measuring actual large-scale chromatin compaction; 2) determine the functional connections between gene positioning to the nuclear lamina and speckles and large-scale chromatin compaction with gene regulation; 3) dissect the relationships between large-scale chromatin folding, chromatin domain epigenetic states, and nuclear compartmentalization with each other and with transcriptional regulation. This work builds on several key advances during the last funding period, including: a) Development of our novel TSA-Seq method for genome-wide mapping of intranuclear gene positioning; b) Demonstration of deterministic positioning of >50% of the most highly expressed genes near nuclear speckles; c) Demonstration of long-range, directed movement of Hsp70 transgenes loci to nuclear speckles upon heat-shock; d) Demonstration of a tight correlation between speckle association and full Hsp70 transgene activation; e) Reconstitution of large chromatin domains with distinct nuclear compartmentalization, epigenetic, and large-scale chromatin condensation states and identification of cis and trans factors which determine these states. Together these advances enable this proposal's unique “Divide-and-Conquer” experimental approach in which we identify trans factors regulating gene positioning and/or large-scale chromatin compaction of particular gene loci and then use genomic methods to survey the genome-wide relevance of these trans factors to gene positioning, large-scale chromatin compaction, and gene regulation. Through this research we will gain a better understanding of gene regulation and how to manipulate gene expression for possible future therapeutic approaches.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2024 ( Subtotal = $335,720 )
2024	2024	UNIVERSITY OF ILLINOIS	506 S WRIGHT ST	URBANA	IL	61801	CHAMPAIGN	USA	Biomedical Research and Research Training	000	23	3/29/2024	NON-COMPETING CONTINUATION	$335,720
														Subtotal = $335,720

Issue Date FY: 2023 ( Subtotal = $373,706 )
2023	2023	UNIVERSITY OF ILLINOIS	506 S WRIGHT ST	URBANA	IL	61801	CHAMPAIGN	USA	Biomedical Research and Research Training	000	22	5/2/2023	NON-COMPETING CONTINUATION	$373,706
2023	2022	UNIVERSITY OF ILLINOIS	506 S WRIGHT ST	URBANA	IL	61801	CHAMPAIGN	USA	Biomedical Research and Research Training	003	21	7/3/2023	COMPETING CONTINUATION	$19,401
2023	2021	UNIVERSITY OF ILLINOIS	506 S WRIGHT ST	URBANA	IL	61801	CHAMPAIGN	USA	Biomedical Research and Research Training	002	20	7/3/2023	NON-COMPETING CONTINUATION	-$4,643
2023	2021	UNIVERSITY OF ILLINOIS	506 S WRIGHT ST	URBANA	IL	61801	CHAMPAIGN	USA	Biomedical Research and Research Training	001	20	5/15/2023	NON-COMPETING CONTINUATION	-$14,758
														Subtotal = $373,706

Issue Date FY: 2022 ( Subtotal = $374,370 )
2022	2022	UNIVERSITY OF ILLINOIS	506 S WRIGHT ST	URBANA	IL	61801	CHAMPAIGN	USA	Biomedical Research and Research Training	001	21	8/1/2022	COMPETING CONTINUATION	$374,370
2022	2021	UNIVERSITY OF ILLINOIS	506 S WRIGHT ST	URBANA	IL	61801	CHAMPAIGN	USA	Biomedical Research and Research Training	000	20	7/31/2022	NON-COMPETING CONTINUATION	$0
														Subtotal = $374,370

Issue Date FY: 2021 ( Subtotal = $379,991 )
2021	2021	UNIVERSITY OF ILLINOIS	364 HENRY ADMINISTRATION BLDG	URBANA	IL	61801	CHAMPAIGN	USA	Biomedical Research and Research Training	000	20	2/24/2021	NON-COMPETING CONTINUATION	$379,991
														Subtotal = $379,991

Issue Date FY: 2020 ( Subtotal = $380,472 )
2020	2020	UNIVERSITY OF ILLINOIS	364 HENRY ADMINISTRATION BLDG	URBANA	IL	61801	CHAMPAIGN	USA	Biomedical Research and Research Training	000	19	2/5/2020	NON-COMPETING CONTINUATION	$342,424
2020	2020	UNIVERSITY OF ILLINOIS	364 HENRY ADMINISTRATION BLDG	URBANA	IL	61801	CHAMPAIGN	USA	Biomedical Research and Research Training	001	19	2/27/2020	NON-COMPETING CONTINUATION	$38,048
														Subtotal = $380,472

Issue Date FY: 2019 ( Subtotal = $380,938 )
2019	2019	UNIVERSITY OF ILLINOIS	506 S WRIGHT ST STE 364	URBANA	IL	61801	CHAMPAIGN	USA	Biomedical Research and Research Training	000	18	12/10/2018	NON-COMPETING CONTINUATION	$380,938
														Subtotal = $380,938

Issue Date FY: 2018 ( Subtotal = $381,392 )
2018	2018	UNIVERSITY OF ILLINOIS	506 S WRIGHT ST STE 364	URBANA	IL	61801	CHAMPAIGN	USA	Biomedical Research and Research Training	000	17	12/7/2017	COMPETING CONTINUATION	$381,392
2018	2014	UNIVERSITY OF ILLINOIS	506 S WRIGHT ST STE 364	URBANA	IL	61801	CHAMPAIGN	USA	Biomedical Research and Research Training	001	16	3/5/2018	NON-COMPETING CONTINUATION	$0
														Subtotal = $381,392

Issue Date FY: 2016 ( Subtotal = $0 )
2016	2015	UNIVERSITY OF ILLINOIS BOARD OF TRUSTEES	109 COBLE HALL, 801 S WRIGHT S	CHAMPAIGN	IL	61820	CHAMPAIGN	USA	Biomedical Research and Research Training	000	16	7/18/2016	SUPPLEMENT FOR EXPANSION	$0
2016	2014	UNIVERSITY OF ILLINOIS BOARD OF TRUSTEES	109 COBLE HALL, 801 S WRIGHT S	CHAMPAIGN	IL	61820	CHAMPAIGN	USA	Biomedical Research and Research Training	001	16	7/18/2016	NON-COMPETING CONTINUATION	$0
														Subtotal = $0

Issue Date FY: 2015 ( Subtotal = $116,267 )
2015	2015	UNIVERSITY OF ILLINOIS BOARD OF TRUSTEES	109 COBLE HALL, 801 S WRIGHT S	CHAMPAIGN	IL	61820	CHAMPAIGN	USA	Biomedical Research and Research Training	001	16	9/15/2015	SUPPLEMENT FOR EXPANSION	$116,267
2015	2014	UNIVERSITY OF ILLINOIS BOARD OF TRUSTEES	109 COBLE HALL, 801 S WRIGHT S	CHAMPAIGN	IL	61820	CHAMPAIGN	USA	Biomedical Research and Research Training	000	16	9/14/2015	NON-COMPETING CONTINUATION	$0
														Subtotal = $116,267

Issue Date FY: 2014 ( Subtotal = $348,615 )
2014	2014	UNIVERSITY OF ILLINOIS BOARD OF TRUSTEES	109 COBLE HALL, 801 S WRIGHT S	CHAMPAIGN	IL	61820	CHAMPAIGN	USA	Biomedical Research and Research Training	000	16	5/30/2014	NON-COMPETING CONTINUATION	$348,615
														Subtotal = $348,615

Issue Date FY: 2013 ( Subtotal = $337,316 )
2013	2013	UNIVERSITY OF ILLINOIS BOARD OF TRUSTEES	109 COBLE HALL, 801 S WRIGHT S	CHAMPAIGN	IL	61820	CHAMPAIGN	USA	Biomedical Research and Research Training	000	15	5/31/2013	NON-COMPETING CONTINUATION	$337,316
														Subtotal = $337,316

Issue Date FY: 2012 ( Subtotal = $350,459 )
2012	2012	UNIVERSITY OF ILLINOIS BOARD OF TRUSTEES	109 COBLE HALL, 801 S WRIGHT S	CHAMPAIGN	IL	61820	CHAMPAIGN	USA	Biomedical Research and Research Training	000	14	5/21/2012	NON-COMPETING CONTINUATION	$350,459
														Subtotal = $350,459

Issue Date FY: 2011 ( Subtotal = $351,340 )
2011	2011	UNIVERSITY OF ILLINOIS BOARD OF TRUSTEES	109 COBLE HALL, 801 S WRIGHT S	CHAMPAIGN	IL	61820	CHAMPAIGN	USA	Biomedical Research and Research Training	000	13	5/31/2011	COMPETING CONTINUATION	$351,340
2011	2010	UNIVERSITY OF ILLINOIS BOARD OF TRUSTEES	109 COBLE HALL, 801 S WRIGHT S	CHAMPAIGN	IL	61820	CHAMPAIGN	USA	Biomedical Research and Research Training	001	12	5/31/2011	NON-COMPETING CONTINUATION	$0
														Subtotal = $351,340

Issue Date FY: 2010 ( Subtotal = $329,662 )
2010	2010	UNIVERSITY OF ILLINOIS BOARD OF TRUSTEES	109 COBLE HALL, 801 S WRIGHT S	CHAMPAIGN	IL	61820	CHAMPAIGN	USA	Biomedical Research and Research Training	000	12	2/24/2010	NON-COMPETING CONTINUATION	$329,662
														Subtotal = $329,662

Issue Date FY: 2009 ( Subtotal = $371,237 )
2009	2009	UNIVERSITY OF ILLINOIS BOARD OF TRUSTEES	109 COBLE HALL, 801 S WRIGHT S	CHAMPAIGN	IL	61820	CHAMPAIGN	USA	Biomedical Research and Research Training	002	11	2/2/2009	SUPPLEMENT FOR EXPANSION	$47,944
2009	2009	UNIVERSITY OF ILLINOIS BOARD OF TRUSTEES	109 COBLE HALL, 801 S WRIGHT S	CHAMPAIGN	IL	61820	CHAMPAIGN	USA	Biomedical Research and Research Training	000	11	1/28/2009	NON-COMPETING CONTINUATION	$290,964
2009	2009	UNIVERSITY OF ILLINOIS BOARD OF TRUSTEES	109 COBLE HALL, 801 S WRIGHT S	CHAMPAIGN	IL	61820	CHAMPAIGN	USA	Biomedical Research and Research Training	001	11	5/12/2009	NON-COMPETING CONTINUATION	$32,329
														Subtotal = $371,237

Issue Date FY: 2008 ( Subtotal = $367,144 )
2008	2008	UNIVERSITY OF ILLINOIS BOARD OF TRUSTEES	109 COBLE HALL, 801 S WRIGHT S	CHAMPAIGN	IL	61820	CHAMPAIGN	USA	Biomedical Research and Research Training	001	10	6/20/2008	SUPPLEMENT FOR EXPANSION	$53,268
2008	2008	UNIVERSITY OF ILLINOIS BOARD OF TRUSTEES	109 COBLE HALL, 801 S WRIGHT S	CHAMPAIGN	IL	61820	CHAMPAIGN	USA	Biomedical Research and Research Training	000	10	3/4/2008	NON-COMPETING CONTINUATION	$313,876
														Subtotal = $367,144

Issue Date FY: 2007 ( Subtotal = $422,849 )
2007	2007	UNIVERSITY OF ILLINOIS BOARD OF TRUSTEES	109 COBLE HALL, 801 S WRIGHT S	CHAMPAIGN	IL	61820	CHAMPAIGN	USA	Biomedical Research and Research Training	001	8	1/29/2007	SUPPLEMENT FOR EXPANSION	$98,794
2007	2007	UNIVERSITY OF ILLINOIS BOARD OF TRUSTEES	109 COBLE HALL, 801 S WRIGHT S	CHAMPAIGN	IL	61820	CHAMPAIGN	USA	Biomedical Research and Research Training	000	9	7/17/2007	COMPETING CONTINUATION	$324,055
														Subtotal = $422,849

Issue Date FY: 2006 ( Subtotal = $296,380 )
2006	2006	UNIVERSITY OF ILLINOIS BOARD OF TRUSTEES	109 COBLE HALL, 801 S WRIGHT S	CHAMPAIGN	IL	61820	CHAMPAIGN	USA	Biomedical Research and Research Training	000	8	1/26/2006	NON-COMPETING CONTINUATION	$296,380
														Subtotal = $296,380

Issue Date FY: 2005 ( Subtotal = $303,697 ) (Continued on the next page)
Grand Total All Awards = $7,905,916

Page 1 of 2 (63 items)12

Top

All Categories

About

Search

Reports

Data Submission

Award Information

Chromatin Domain Structure and Function

Award Number: R01GM058460

ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

Federal Websites

Department of Health & Human Services

HHS Operating Divisions

HHS Staff Divisions

Download A Document Viewer