Saturday, December 21, 2024 12/21/2024

Protein RNA Rearrangements in the Spliceosome

Award Number: R01GM057829
ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

Group Awards By:

View Award Abstract

ABSTRACT Excision of introns from precursor messenger RNA by the spliceosome is a critical step in almost all human gene expression. This process is highly regulated, integrally linked with the transcription of genes and other processing events, such as polyadenylation and nucleotide modification. The mechanism by which the spliceosome recognizes the exact sites for the chemical events and how the reactions are catalyzed are not well understood. The long-term goals of this project are to understand interactions and rearrangements between spliceosome components and the RNA ligands that are substrates for the catalytic reactions. Ample evidence argues for multiple rearrangements of factors and multiple recognition events at the branch site. Investigation of these events — which are not understood mechanistically — will elucidate interactions and rearrangements among core components and may serve as a paradigm for rearrangements in the spliceosome and in other RNP machines. This proposal focuses on mechanisms by which spliceosomal dynamics impact splicing fidelity. The Aims of this project focus on two newly-discovered aspects of the spliceosome and snRNAs: (1) We will investigate the role of inositol hexakisphosphate (IP6) in spliceosome function. This small molecule was recently identified unexpectedly by cryo-EM as part of splicing complexes. No biochemistry or genetics has so far explored its importance. We use mutants in the IP6 biosynthetic pathway to explore its contribution to splicing and whether inositol polyphosphate (IPP) isoform levels may influence splice events. (2) One hole in our current understanding of RNA biology is the role of snRNA mutations in disease. Two mutations were recently described in the 5'-end of U1 snRNA, associated with glioblastoma (GBM) and other cancers. We will use our in silico snRNA genome to interrogate TCGA whole genome datasets (WGS) and investigate, in particular, U2 snRNA mutations that we find highly enriched in bladder cancer and bladder cancer organoid lines.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2024 ( Subtotal = $554,371 )
2024	2024	ALBERT EINSTEIN COLLEGE OF MEDICINE	1300 MORRIS PARK AVE	BRONX	NY	10461	BRONX	USA	Biomedical Research and Research Training	000	26	1/5/2024	NON-COMPETING CONTINUATION	$498,935
2024	2024	ALBERT EINSTEIN COLLEGE OF MEDICINE	1300 MORRIS PARK AVE	BRONX	NY	10461	BRONX	USA	Biomedical Research and Research Training	001	26	6/20/2024	NON-COMPETING CONTINUATION	$55,436
														Subtotal = $554,371

Issue Date FY: 2023 ( Subtotal = $554,371 )
2023	2023	ALBERT EINSTEIN COLLEGE OF MEDICINE	1300 MORRIS PARK AVE	BRONX	NY	10461	BRONX	USA	Biomedical Research and Research Training	001	25	2/14/2023	NON-COMPETING CONTINUATION	$55,436
2023	2023	ALBERT EINSTEIN COLLEGE OF MEDICINE	1300 MORRIS PARK AVE	BRONX	NY	10461	BRONX	USA	Biomedical Research and Research Training	000	25	12/22/2022	NON-COMPETING CONTINUATION	$498,935
														Subtotal = $554,371

Issue Date FY: 2022 ( Subtotal = $554,371 )
2022	2022	ALBERT EINSTEIN COLLEGE OF MEDICINE	1300 MORRIS PARK AVE	BRONX	NY	10461	BRONX	USA	Biomedical Research and Research Training	002	24	1/21/2022	COMPETING CONTINUATION	$554,371
2022	2022	ALBERT EINSTEIN COLLEGE OF MEDICINE	1300 MORRIS PARK AVE	BRONX	NY	10461	BRONX	USA	Biomedical Research and Research Training	004	24	7/29/2022	COMPETING CONTINUATION	$1,508
2022	2020	ALBERT EINSTEIN COLLEGE OF MEDICINE	1300 MORRIS PARK AVE	BRONX	NY	10461	BRONX	USA	Biomedical Research and Research Training	000	23	1/20/2022	NON-COMPETING CONTINUATION	$0
2022	2020	ALBERT EINSTEIN COLLEGE OF MEDICINE	1300 MORRIS PARK AVE	BRONX	NY	10461	BRONX	USA	Biomedical Research and Research Training	001	23	1/21/2022	NON-COMPETING CONTINUATION	$0
2022	2020	ALBERT EINSTEIN COLLEGE OF MEDICINE	1300 MORRIS PARK AVE	BRONX	NY	10461	BRONX	USA	Biomedical Research and Research Training	003	23	7/29/2022	SUPPLEMENT FOR EXPANSION	-$1,508
														Subtotal = $554,371

Issue Date FY: 2020 ( Subtotal = $598,506 )
2020	2020	ALBERT EINSTEIN COLLEGE OF MEDICINE	1300 MORRIS PARK AVE	BRONX	NY	10461	BRONX	USA	Biomedical Research and Research Training	000	23	2/13/2020	NON-COMPETING CONTINUATION	$535,021
2020	2020	ALBERT EINSTEIN COLLEGE OF MEDICINE	1300 MORRIS PARK AVE	BRONX	NY	10461	BRONX	USA	Biomedical Research and Research Training	001	23	6/24/2020	SUPPLEMENT FOR EXPANSION	$63,485
														Subtotal = $598,506

Issue Date FY: 2019 ( Subtotal = $535,021 )
2019	2019	ALBERT EINSTEIN COLLEGE OF MEDICINE	1300 MORRIS PARK AVE	BRONX	NY	10461	BRONX	USA	Biomedical Research and Research Training	003	22	2/21/2019	NON-COMPETING CONTINUATION	$535,021
2019	2018	ALBERT EINSTEIN COLLEGE OF MEDICINE	1300 MORRIS PARK AVE	BRONX	NY	10461	BRONX	USA	Biomedical Research and Research Training	006	21	8/7/2019	CHANGE OF GRANTEE / TRAINING INSTITUTION / AWARDING INSTITUTION	$120,191
2019	2018	ALBERT EINSTEIN COLLEGE OF MEDICINE	1300 MORRIS PARK AVE	BRONX	NY	10461	BRONX	USA	Biomedical Research and Research Training	005	21	5/16/2019	CHANGE OF GRANTEE / TRAINING INSTITUTION / AWARDING INSTITUTION	$16,517
2019	2018	ALBERT EINSTEIN COLLEGE OF MEDICINE	1300 MORRIS PARK AVE	BRONX	NY	10461	BRONX	USA	Biomedical Research and Research Training	002	21	2/8/2019	CHANGE OF GRANTEE / TRAINING INSTITUTION / AWARDING INSTITUTION	$50,664
2019	2018	ALBERT EINSTEIN COLLEGE OF MEDICINE, INC.	1300 MORRIS PARK AVE	BRONX	NY	10461	BRONX	USA	Biomedical Research and Research Training	001	20	2/7/2019	SUPPLEMENT FOR EXPANSION	-$120,191
2019	2018	ALBERT EINSTEIN COLLEGE OF MEDICINE, INC.	1300 MORRIS PARK AVE	BRONX	NY	10461	BRONX	USA	Biomedical Research and Research Training	004	20	5/16/2019	NON-COMPETING CONTINUATION	-$16,517
2019	2018	ALBERT EINSTEIN COLLEGE OF MEDICINE, INC.	1300 MORRIS PARK AVE	BRONX	NY	10461	BRONX	USA	Biomedical Research and Research Training	000	20	2/6/2019	NON-COMPETING CONTINUATION	-$50,664
														Subtotal = $535,021

Issue Date FY: 2018 ( Subtotal = $655,212 )
2018	2018	ALBERT EINSTEIN COLLEGE OF MEDICINE, INC.	1300 MORRIS PARK AVE	BRONX	NY	10461	BRONX	USA	Biomedical Research and Research Training	000	20	2/19/2018	NON-COMPETING CONTINUATION	$481,519
2018	2018	ALBERT EINSTEIN COLLEGE OF MEDICINE, INC.	1300 MORRIS PARK AVE	BRONX	NY	10461	BRONX	USA	Biomedical Research and Research Training	002	20	9/19/2018	SUPPLEMENT FOR EXPANSION	$120,191
2018	2018	ALBERT EINSTEIN COLLEGE OF MEDICINE, INC.	1300 MORRIS PARK AVE	BRONX	NY	10461	BRONX	USA	Biomedical Research and Research Training	001	20	5/31/2018	NON-COMPETING CONTINUATION	$53,502
														Subtotal = $655,212

Issue Date FY: 2017 ( Subtotal = $535,021 )
2017	2017	ALBERT EINSTEIN COLLEGE OF MEDICINE, INC.	1300 MORRIS PARK AVE	BRONX	NY	10461	BRONX	USA	Biomedical Research and Research Training	001	19	5/19/2017	COMPETING CONTINUATION	$535,021
2017	2015	ALBERT EINSTEIN COLLEGE OF MEDICINE, INC.	1300 MORRIS PARK AVE	BRONX	NY	10461	BRONX	USA	Biomedical Research and Research Training	000	18	5/16/2017	CHANGE OF GRANTEE / TRAINING INSTITUTION / AWARDING INSTITUTION	$0
2017	2015	ALBERT EINSTEIN COLLEGE OF MEDICINE, INC.	1300 MORRIS PARK AVE	BRONX	NY	10461	BRONX	USA	Biomedical Research and Research Training	002	18	9/9/2017	CHANGE OF GRANTEE / TRAINING INSTITUTION / AWARDING INSTITUTION	$0
														Subtotal = $535,021

Issue Date FY: 2016 ( Subtotal = $0 )
2016	2015	ALBERT EINSTEIN COLLEGE OF MEDICINE INC	111 E 210TH ST	BRONX	NY	10467	BRONX	USA	Biomedical Research and Research Training	001	18	12/11/2015	CHANGE OF GRANTEE / TRAINING INSTITUTION / AWARDING INSTITUTION	$460,326
2016	2015	ALBERT EINSTEIN COLLEGE OF MEDICINE INC	111 E 210TH ST	BRONX	NY	10467	BRONX	USA	Biomedical Research and Research Training	003	18	5/27/2016	CHANGE OF GRANTEE / TRAINING INSTITUTION / AWARDING INSTITUTION	-$3,924
2016	2015	ALBERT EINSTEIN COLLEGE OF MEDICINE OF YESHIVA UNIV	1300 MORRIS PARK AVENUE	BRONIX	NY	10461		USA	Biomedical Research and Research Training	002	17	5/26/2016	NON-COMPETING CONTINUATION	$3,924
2016	2015	ALBERT EINSTEIN COLLEGE OF MEDICINE OF YESHIVA UNIV	1300 MORRIS PARK AVENUE	BRONIX	NY	10461		USA	Biomedical Research and Research Training	000	17	12/10/2015	NON-COMPETING CONTINUATION	-$460,326
														Subtotal = $0

Issue Date FY: 2015 ( Subtotal = $511,402 )
2015	2015	ALBERT EINSTEIN COLLEGE OF MEDICINE OF YESHIVA UNIV	1300 MORRIS PARK AVENUE	BRONIX	NY	10461		USA	Biomedical Research and Research Training	000	17	7/24/2015	NON-COMPETING CONTINUATION	$511,402
														Subtotal = $511,402

Issue Date FY: 2014 ( Subtotal = $546,692 )
2014	2014	ALBERT EINSTEIN COLLEGE OF MEDICINE OF YESHIVA UNIV	1300 MORRIS PARK AVENUE	BRONIX	NY	10461		USA	Biomedical Research and Research Training	000	16	7/21/2014	NON-COMPETING CONTINUATION	$546,692
														Subtotal = $546,692

Issue Date FY: 2013 ( Subtotal = $596,903 )
2013	2013	ALBERT EINSTEIN COLLEGE OF MEDICINE OF YESHIVA UNIV	1300 MORRIS PARK AVENUE	BRONIX	NY	10461		USA	Biomedical Research and Research Training	000	14	2/25/2013	SUPPLEMENT FOR EXPANSION	$35,290
2013	2013	ALBERT EINSTEIN COLLEGE OF MEDICINE OF YESHIVA UNIV	1300 MORRIS PARK AVENUE	BRONIX	NY	10461		USA	Biomedical Research and Research Training	001	15	7/29/2013	NON-COMPETING CONTINUATION	$561,613
														Subtotal = $596,903

Issue Date FY: 2012 ( Subtotal = $511,402 )
2012	2012	ALBERT EINSTEIN COLLEGE OF MEDICINE OF YESHIVA UNIV	1300 MORRIS PARK AVENUE	BRONIX	NY	10461		USA	Biomedical Research and Research Training	000	14	7/30/2012	COMPETING CONTINUATION	$511,402
														Subtotal = $511,402

Issue Date FY: 2011 ( Subtotal = $472,690 )
2011	2011	ALBERT EINSTEIN COLLEGE OF MEDICINE OF YESHIVA UNIV	1300 MORRIS PARK AVENUE	BRONIX	NY	10461		USA	Biomedical Research and Research Training	000	13	7/20/2011	NON-COMPETING CONTINUATION	$472,690
														Subtotal = $472,690

Issue Date FY: 2010 ( Subtotal = $477,544 )
2010	2010	ALBERT EINSTEIN COLLEGE OF MEDICINE OF YESHIVA UNIV	1300 MORRIS PARK AVENUE	BRONIX	NY	10461		USA	Biomedical Research and Research Training	000	12	7/13/2010	NON-COMPETING CONTINUATION	$477,544
														Subtotal = $477,544

Issue Date FY: 2009 ( Subtotal = $468,401 ) (Continued on the next page)
Grand Total All Awards = $11,139,172

Page 1 of 2 (71 items)12

Top

All Categories

About

Search

Reports

Data Submission

Award Information

Protein RNA Rearrangements in the Spliceosome

Award Number: R01GM057829

ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

Federal Websites

Department of Health & Human Services

HHS Operating Divisions

HHS Staff Divisions

Download A Document Viewer