Sunday, May 11, 2025 5/11/2025

Molecular and functional dissection of the zebrafish hematopoietic stem cell niche

Award Number: R01DK074482
ORGANIZATION: NATIONAL INSTITUTE OF DIABETES & DIGESTIVE & KIDNEY DISEASES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

Group Awards By:

View Award Description

Molecular and functional dissection of the zebrafish hematopoietic stem cell niche - Project Summary/Abstract: Hematopoietic stem cells (HSCs) give rise to all terminally differentiated cells in the blood. The ability of HSCs to reconstitute these blood cell lineages for life underlies the efficacy of bone marrow transplantation therapy for treatment of various blood disorders, including leukemias, anemia, and autoimmunity. Although this is an established and effective treatment, two-thirds of patients in need of a transplant lack a matched donor. Therefore, alternative sources of therapeutic HSCs would be a boon to the field. Human pluripotent stem cells (hPSCs) represent a potential source for cell-based therapies, including the derivation of patient-specific transplantable HSCs, which would additionally circumvent immune rejection and alloreactivity, both major issues in the clinic. The goal of the proposed research is to extend our preliminary observations on a novel population of somite- derived endothelial cells (SDECs). Of note, these cells migrate exclusively to the nascent dorsal aorta and act as a developmental niche for the subsequent emergence of HSCs. Characterization of SDECs will provide a deeper understanding of the cues that govern HSC development in vivo. The fundamental discoveries made in the course of these studies will ultimately inform derivation strategies of HSCs from hPSCs. This proposal will leverage lineage tracing methods in zebrafish and in vitro differentiation protocols of hPSCs combined with single-cell sequencing approaches to determine the molecular mechanisms of this requirement, and to provide a new level of understanding of how posterior lateral mesoderm is instructed to generate HSCs. The long-term goal of these studies is to gain a better understanding of how HSCs develop in the embryo in order to translate this information to hPSCs. Successful completion of this research will have a profound impact on HSC derivation and expansion, and thereby will be instrumental in overcoming current obstacles to the effective treatment of diseases requiring bone marrow transplant therapy.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2025 ( Subtotal = -$26,766 )
2025	2024	UNIVERSITY OF CALIFORNIA, SAN DIEGO	9500 GILMAN DR	LA JOLLA	CA	92093	SAN DIEGO	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	000	18	4/22/2025	NON-COMPETING CONTINUATION	-$26,766
														Subtotal = -$26,766

Issue Date FY: 2024 ( Subtotal = $406,941 )
2024	2024	UNIVERSITY OF CALIFORNIA, SAN DIEGO	9500 GILMAN DR	LA JOLLA	CA	92093	SAN DIEGO	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	001	18	5/1/2024	NON-COMPETING CONTINUATION	$25,434
2024	2024	UNIVERSITY OF CALIFORNIA, SAN DIEGO	9500 GILMAN DRIVE	LA JOLLA	CA	92093	SAN DIEGO	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	000	18	11/20/2023	NON-COMPETING CONTINUATION	$381,507
														Subtotal = $406,941

Issue Date FY: 2023 ( Subtotal = $376,791 )
2023	2023	UNIVERSITY OF CALIFORNIA, SAN DIEGO	9500 GILMAN DR	LA JOLLA	CA	92093	SAN DIEGO	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	000	17	2/3/2023	NON-COMPETING CONTINUATION	$376,791
														Subtotal = $376,791

Issue Date FY: 2022 ( Subtotal = $377,333 )
2022	2022	UNIVERSITY OF CALIFORNIA, SAN DIEGO	9500 GILMAN DR	LA JOLLA	CA	92093	SAN DIEGO	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	001	16	3/29/2022	NON-COMPETING CONTINUATION	$37,733
2022	2022	UNIVERSITY OF CALIFORNIA, SAN DIEGO	9500 GILMAN DR	LA JOLLA	CA	92093	SAN DIEGO	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	000	16	12/9/2021	NON-COMPETING CONTINUATION	$339,600
														Subtotal = $377,333

Issue Date FY: 2021 ( Subtotal = $373,374 )
2021	2021	UNIVERSITY OF CALIFORNIA, SAN DIEGO	9500 GILMAN DR	LA JOLLA	CA	92093	SAN DIEGO	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	002	15	7/9/2021	COMPETING CONTINUATION	$26,766
2021	2021	UNIVERSITY OF CALIFORNIA, SAN DIEGO	9500 GILMAN DR	LA JOLLA	CA	92093	SAN DIEGO	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	000	15	11/24/2020	COMPETING CONTINUATION	$373,374
2021	2019	UNIVERSITY OF CALIFORNIA, SAN DIEGO	9500 GILMAN DR	LA JOLLA	CA	92093	SAN DIEGO	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	001	14	7/9/2021	NON-COMPETING CONTINUATION	-$26,766
														Subtotal = $373,374

Issue Date FY: 2019 ( Subtotal = $331,946 )
2019	2019	UNIVERSITY OF CALIFORNIA, SAN DIEGO	9500 GILMAN DR DEPT 621	LA JOLLA	CA	92093	SAN DIEGO	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	000	14	8/26/2019	NON-COMPETING CONTINUATION	$331,946
														Subtotal = $331,946

Issue Date FY: 2018 ( Subtotal = $420,547 )
2018	2018	UNIVERSITY OF CALIFORNIA, SAN DIEGO	9500 GILMAN DR DEPT 621	LA JOLLA	CA	92093	SAN DIEGO	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	000	13	8/20/2018	NON-COMPETING CONTINUATION	$420,547
														Subtotal = $420,547

Issue Date FY: 2017 ( Subtotal = $422,154 )
2017	2017	UNIVERSITY OF CALIFORNIA, SAN DIEGO	9500 GILMAN DR DEPT 621	LA JOLLA	CA	92093	SAN DIEGO	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	001	12	9/12/2017	SUPPLEMENT FOR EXPANSION	$86,800
2017	2017	UNIVERSITY OF CALIFORNIA, SAN DIEGO	9500 GILMAN DR DEPT 621	LA JOLLA	CA	92093	SAN DIEGO	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	000	12	8/8/2017	NON-COMPETING CONTINUATION	$335,354
														Subtotal = $422,154

Issue Date FY: 2016 ( Subtotal = $336,789 )
2016	2016	UNIVERSITY OF CALIFORNIA-SAN DIEGO	9500 GILMAN DRIVE	LA JOLLA	CA	92093	SAN DIEGO	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	000	11	8/4/2016	NON-COMPETING CONTINUATION	$336,789
														Subtotal = $336,789

Issue Date FY: 2015 ( Subtotal = $338,071 )
2015	2015	UNIVERSITY OF CALIFORNIA-SAN DIEGO	9500 GILMAN DRIVE	LA JOLLA	CA	92093	SAN DIEGO	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	000	10	5/12/2015	COMPETING CONTINUATION	$338,071
														Subtotal = $338,071

Issue Date FY: 2014 ( Subtotal = $407,685 )
2014	2014	UNIVERSITY OF CALIFORNIA-SAN DIEGO	9500 GILMAN DRIVE	LA JOLLA	CA	92093	SAN DIEGO	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	000	8	3/28/2014	SUPPLEMENT FOR EXPANSION	$80,408
2014	2014	UNIVERSITY OF CALIFORNIA-SAN DIEGO	9500 GILMAN DRIVE	LA JOLLA	CA	92093	SAN DIEGO	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	001	9	8/19/2014	NON-COMPETING CONTINUATION	$327,277
														Subtotal = $407,685

Issue Date FY: 2013 ( Subtotal = $316,686 )
2013	2013	UNIVERSITY OF CALIFORNIA-SAN DIEGO	9500 GILMAN DRIVE	LA JOLLA	CA	92093	SAN DIEGO	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	000	8	7/30/2013	NON-COMPETING CONTINUATION	$316,686
														Subtotal = $316,686

Issue Date FY: 2012 ( Subtotal = $328,986 )
2012	2012	UNIVERSITY OF CALIFORNIA-SAN DIEGO	9500 GILMAN DRIVE	LA JOLLA	CA	92093	SAN DIEGO	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	000	7	8/14/2012	NON-COMPETING CONTINUATION	$328,986
														Subtotal = $328,986

Issue Date FY: 2011 ( Subtotal = $328,961 )
2011	2011	UNIVERSITY OF CALIFORNIA-SAN DIEGO	9500 GILMAN DRIVE	LA JOLLA	CA	92093	SAN DIEGO	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	001	6	9/9/2011	COMPETING CONTINUATION	$328,961
2011	2010	UNIVERSITY OF CALIFORNIA-SAN DIEGO	9500 GILMAN DRIVE	LA JOLLA	CA	92093	SAN DIEGO	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	000	5	9/8/2011	NON-COMPETING CONTINUATION	$0
														Subtotal = $328,961

Issue Date FY: 2010 ( Subtotal = $390,842 )
2010	2010	UNIVERSITY OF CALIFORNIA-SAN DIEGO	9500 GILMAN DRIVE	LA JOLLA	CA	92093	SAN DIEGO	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	001	5	4/29/2010	NON-COMPETING CONTINUATION	$290,843
2010	2010	UNIVERSITY OF CALIFORNIA-SAN DIEGO	9500 GILMAN DRIVE	LA JOLLA	CA	92093	SAN DIEGO	USA	Trans-NIH Recovery Act Research Support	000	4	1/18/2010	SUPPLEMENT FOR EXPANSION	$99,999
														Subtotal = $390,842

Issue Date FY: 2009 ( Subtotal = $296,018 )
2009	2009	UNIVERSITY OF CALIFORNIA-SAN DIEGO	9500 GILMAN DRIVE	LA JOLLA	CA	92093	SAN DIEGO	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	000	3	3/17/2009	SUPPLEMENT FOR EXPANSION	$1,545
2009	2009	UNIVERSITY OF CALIFORNIA-SAN DIEGO	9500 GILMAN DRIVE	LA JOLLA	CA	92093	SAN DIEGO	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	001	4	6/5/2009	NON-COMPETING CONTINUATION	$294,473
														Subtotal = $296,018

Issue Date FY: 2008 ( Subtotal = $328,768 )
2008	2008	UNIVERSITY OF CALIFORNIA-SAN DIEGO	9500 GILMAN DRIVE	LA JOLLA	CA	92093	SAN DIEGO	USA	Kidney Diseases, Urology and Hematology Research	000	3	3/27/2008	NON-COMPETING CONTINUATION	$328,768
														Subtotal = $328,768

Issue Date FY: 2007 ( Subtotal = $370,716 )
2007	2007	UNIVERSITY OF CALIFORNIA-SAN DIEGO	9500 GILMAN DRIVE	LA JOLLA	CA	92093	SAN DIEGO	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	000	2	4/5/2007	NON-COMPETING CONTINUATION	$370,716
														Subtotal = $370,716

Issue Date FY: 2006 ( Subtotal = $336,427 )
2006	2006	UNIVERSITY OF CALIFORNIA-SAN DIEGO	9500 GILMAN DRIVE	LA JOLLA	CA	92093	SAN DIEGO	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	001	1	9/18/2006	SUPPLEMENT FOR EXPANSION	$38,783
2006	2006	UNIVERSITY OF CALIFORNIA-SAN DIEGO	9500 GILMAN DRIVE	LA JOLLA	CA	92093	SAN DIEGO	USA	Kidney Diseases, Urology and Hematology Research	000	1	5/8/2006	NEW	$297,644
														Subtotal = $336,427

Grand Total All Awards = $6,462,269

Top

All Categories

About

Search

Reports

Data Submission

Award Information

Molecular and functional dissection of the zebrafish hematopoietic stem cell niche

Award Number: R01DK074482

ORGANIZATION: NATIONAL INSTITUTE OF DIABETES & DIGESTIVE & KIDNEY DISEASES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

Federal Websites

Department of Health & Human Services

HHS Operating Divisions

HHS Staff Divisions

Download A Document Viewer