Thursday, November 21, 2024 11/21/2024

Analysis of Cellular Plasticity in White Adipose Tissue.

Award Number: R01DK062292
ORGANIZATION: NATIONAL INSTITUTE OF DIABETES & DIGESTIVE & KIDNEY DISEASES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

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ABSTRACT The long-term goal of this project is to understand the cellular and molecular mechanisms of adipose tissue establishment, maintenance, and pathophysiological remodeling. Our central hypothesis is that adipose tissue plasticity involves the creation and resolution of dynamic adipogenic niches involving close interactions among specific stromal and immune cell subtypes. While single cell RNA sequencing (scRNA-seq) has identified the stromal and immune phenotypes that are involved in adipose tissue neogenesis, how these cells are recruited to specific locations and interact to create and resolve adipogenic niches has not been addressed. In this continuation we will use cutting edge high-dimension, high-resolution spatial transcriptomics and proteomics to map, order, and test the cellular events underlying adipose tissue neogenesis. Despite recent advances in single cell profiling, the origins of adipocytes and lineage relationships of stromal cells in adipose tissues remains controversial. To address this critical gap in knowledge we will apply newly- developed CRISPR/Cas9-based technologies for unbiased, high-dimension resolution of cell lineages during the life cycle of adipose tissues. This technology will be integrated with complementary techniques of spatial transcriptomics, multiplexed 3-dimensional single molecule fluorescence in situ hybridization and immunofluorescence. This integrated approach will provide comprehensive, unbiased spatial analysis of lineage relationships in postnatal adipose tissue establishment, expansion, aging, and remodeling in vivo. Successful completion of this proposal will provide the first unbiased high-dimension, single-cell resolution map of mouse adipose tissues during establishment, expansion, and aging. We will determine the cellular and molecular mechanisms of adipogenic niche formation and resolution, and resolve long-standing questions of cell lineages in a comprehensive and unbiased fashion.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2024 ( Subtotal = $591,795 )
2024	2024	WAYNE STATE UNIVERSITY	5700 CASS AVE STE 4900	DETROIT	MI	48202	WAYNE	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	001	18	6/19/2024	NON-COMPETING CONTINUATION	$552,000
2024	2023	WAYNE STATE UNIVERSITY	5700 CASS AVE STE 4900	DETROIT	MI	48202	WAYNE	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	000	17	1/24/2024	COMPETING CONTINUATION	$39,795
														Subtotal = $591,795

Issue Date FY: 2023 ( Subtotal = $149,041 )
2023	2023	WAYNE STATE UNIVERSITY	5700 CASS AVE STE 4900	DETROIT	MI	48202	WAYNE	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	000	17	8/23/2023	COMPETING CONTINUATION	$605,997
2023	2021	WAYNE STATE UNIVERSITY	5700 CASS AVE STE 4900	DETROIT	MI	48202	WAYNE	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	001	16	9/11/2023	NON-COMPETING CONTINUATION	-$456,956
														Subtotal = $149,041

Issue Date FY: 2022 ( Subtotal = $0 )
2022	2021	WAYNE STATE UNIVERSITY	5057 WOODWARD STE 13001	DETROIT	MI	48202	WAYNE	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	000	16	9/8/2022	NON-COMPETING CONTINUATION	$0
														Subtotal = $0

Issue Date FY: 2021 ( Subtotal = $457,380 )
2021	2021	WAYNE STATE UNIVERSITY	5057 WOODWARD STE 13001	DETROIT	MI	48202	WAYNE	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	000	16	2/12/2021	NON-COMPETING CONTINUATION	$457,380
														Subtotal = $457,380

Issue Date FY: 2020 ( Subtotal = $457,380 )
2020	2020	WAYNE STATE UNIVERSITY	5057 WOODWARD STE 13001	DETROIT	MI	48202	WAYNE	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	000	15	2/12/2020	NON-COMPETING CONTINUATION	$457,380
														Subtotal = $457,380

Issue Date FY: 2019 ( Subtotal = $457,380 )
2019	2019	WAYNE STATE UNIVERSITY	5057 WOODWARD 13 FLOOR	DETROIT	MI	48202	WAYNE	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	000	14	2/11/2019	NON-COMPETING CONTINUATION	$457,380
														Subtotal = $457,380

Issue Date FY: 2018 ( Subtotal = $457,380 )
2018	2018	WAYNE STATE UNIVERSITY	5057 WOODWARD 13 FLOOR	DETROIT	MI	48202	WAYNE	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	001	13	5/10/2018	COMPETING CONTINUATION	$457,380
2018	2018	WAYNE STATE UNIVERSITY	5057 WOODWARD 13 FLOOR	DETROIT	MI	48202	WAYNE	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	003	13	8/10/2018	COMPETING CONTINUATION	$28,809
2018	2016	WAYNE STATE UNIVERSITY	5057 WOODWARD 13 FLOOR	DETROIT	MI	48202	WAYNE	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	000	12	5/7/2018	EXTENSION WITH OR WITHOUT FUNDS	$0
2018	2016	WAYNE STATE UNIVERSITY	5057 WOODWARD 13 FLOOR	DETROIT	MI	48202	WAYNE	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	002	12	8/10/2018	EXTENSION WITH OR WITHOUT FUNDS	-$28,809
														Subtotal = $457,380

Issue Date FY: 2016 ( Subtotal = $330,600 )
2016	2016	WAYNE STATE UNIVERSITY	5700 CASS SUITE 4602	DETROIT	MI	48202	WAYNE	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	000	12	6/10/2016	EXTENSION WITH OR WITHOUT FUNDS	$330,600
														Subtotal = $330,600

Issue Date FY: 2015 ( Subtotal = $330,600 )
2015	2015	WAYNE STATE UNIVERSITY	5700 CASS SUITE 4602	DETROIT	MI	48202	WAYNE	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	000	11	6/11/2015	NON-COMPETING CONTINUATION	$330,600
														Subtotal = $330,600

Issue Date FY: 2014 ( Subtotal = $330,600 )
2014	2014	WAYNE STATE UNIVERSITY	5700 CASS SUITE 4602	DETROIT	MI	48202	WAYNE	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	000	10	6/17/2014	NON-COMPETING CONTINUATION	$330,600
														Subtotal = $330,600

Issue Date FY: 2013 ( Subtotal = $319,030 )
2013	2013	WAYNE STATE UNIVERSITY	5700 CASS SUITE 4602	DETROIT	MI	48202	WAYNE	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	000	9	6/24/2013	NON-COMPETING CONTINUATION	$319,030
														Subtotal = $319,030

Issue Date FY: 2012 ( Subtotal = $330,600 )
2012	2012	WAYNE STATE UNIVERSITY	5700 CASS SUITE 4602	DETROIT	MI	48202	WAYNE	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	000	8	6/8/2012	COMPETING CONTINUATION	$330,600
														Subtotal = $330,600

Issue Date FY: 2010 ( Subtotal = $290,650 )
2010	2010	WAYNE STATE UNIVERSITY	5700 CASS SUITE 4602	DETROIT	MI	48202	WAYNE	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	000	7	7/5/2010	NON-COMPETING CONTINUATION	$290,650
														Subtotal = $290,650

Issue Date FY: 2009 ( Subtotal = $293,586 )
2009	2009	WAYNE STATE UNIVERSITY	5700 CASS SUITE 4602	DETROIT	MI	48202	WAYNE	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	001	6	6/16/2009	NON-COMPETING CONTINUATION	$293,586
2009	2006	WAYNE STATE UNIVERSITY	5700 CASS SUITE 4602	DETROIT	MI	48202	WAYNE	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	000	3	4/6/2009	COMPETING CONTINUATION	$0
														Subtotal = $293,586

Issue Date FY: 2008 ( Subtotal = $293,586 )
2008	2008	WAYNE STATE UNIVERSITY	5700 CASS SUITE 4602	DETROIT	MI	48202	WAYNE	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	000	5	6/26/2008	NON-COMPETING CONTINUATION	$293,586
														Subtotal = $293,586

Issue Date FY: 2007 ( Subtotal = $299,578 )
2007	2007	WAYNE STATE UNIVERSITY	5700 CASS SUITE 4602	DETROIT	MI	48202	WAYNE	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	000	4	5/28/2007	NON-COMPETING CONTINUATION	$299,578
														Subtotal = $299,578

Issue Date FY: 2006 ( Subtotal = $308,525 )
2006	2006	WAYNE STATE UNIVERSITY	5700 CASS SUITE 4602	DETROIT	MI	48202	WAYNE	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	000	3	7/18/2006	COMPETING CONTINUATION	$308,525
														Subtotal = $308,525

Issue Date FY: 2004 ( Subtotal = $113,250 )
2004	2004	WAYNE STATE UNIVERSITY	5700 CASS SUITE 4602	DETROIT	MI	48202	WAYNE	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	000	2	3/22/2004	NON-COMPETING CONTINUATION	$113,250
														Subtotal = $113,250

Issue Date FY: 2003 ( Subtotal = $339,750 )
2003	2003	WAYNE STATE UNIVERSITY	5700 CASS SUITE 4602	DETROIT	MI	48202	WAYNE	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	000	1		NEW	$339,750
														Subtotal = $339,750

Grand Total All Awards = $6,150,710

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Analysis of Cellular Plasticity in White Adipose Tissue.

Award Number: R01DK062292

ORGANIZATION: NATIONAL INSTITUTE OF DIABETES & DIGESTIVE & KIDNEY DISEASES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

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