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Mechanisms that couple irregular development of fetal melanoblasts to premature exhaustion of adult melanocyte stem cells

Award Number: R01AR078559
ORGANIZATION: NATIONAL INSTITUTE OF ARTHRITIS & MUSCULOSKELETAL & SKIN DISEASES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

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Mechanisms that couple irregular development of fetal melanoblasts to premature exhaustion of adult melanocyte stem cells - PROJECT SUMMARY Maintenance of tissue function during adulthood, and hence suppression of tissue degeneration and disease, depends on maintenance of stem cell populations. Adult stem cells are epigenetically programmed and a culmination of a series of developmental decisions initiated in the embryo. In utero environmental exposures on the embryo can influence adult and late-life disease, likely in part via effects on stem cell development that are transmitted to maintenance and function of stem cells in the adult. However, the molecular links between embryonic development and long-term maintenance of stem cell function and phenotype in adults are poorly defined. We will employ lineage-specific genetic inactivation of a histone chaperone to understand how embryonic developmental integrity of melanoblasts (Mb) impacts on maintenance adult melanocyte (Mc) stem cells (McSC). The histone chaperone HIRA deposits histone variant H3.3 into active genes, promoters and enhancers. Through in vitro and in vivo studies and single cell RNA-seq of mouse embryo melanoblasts (Mbs) from wild type mice and mice lacking expression of HIRA in embryonic Mbs, we have uncovered a role for HIRA in sustaining the PAX3/SOX10-MITF Mb specification pathway. Inactivation of HIRA in Mbs depletes the number of Mbs in early/mid stage embryos. However, this embryonic defect is rescued by birth and young mice exhibit normal numbers of melanocytic cells, and only a very subtle pigmentation defect. Nevertheless, in new-born mice, Hira knock out (KO) melanocytic cells exhibit a higher frequency of telomere-associated DNA damage foci, indicating that Hira knock out McSC and/or melanocytes harbor molecular damage, even in new-born mice. Indeed, melanoblasts and melanocytes from new-born Hira KO mice respond poorly to pro-proliferative challenge in vitro and in vivo, and these mice show marked accelerated McSC and melanocyte depletion and dramatically accelerated hair greying during adulthood. Building on these extensive preliminary data, we will investigate the role of HIRA in differentiation and development of the melanocytic lineage, and investigate the links between abnormal embryonic development and adult stem cell depletion during adulthood and aging. Dysregulation of the PAX3/SOX10-MITF signaling pathway contributes to developmental disorders and melanoma. These studies to define HIRA's role in the PAX3/SOX10-MITF axis can promote therapeutic interventions to combat these developmental and neoplastic disorders. Moreover, completion of these Specific Aims will address how the integrity of embryonic development of tissue specific stem cells impacts maintenance of those stem cells during adulthood.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2024 ( Subtotal = $676,107 )
2024	2024	SANFORD BURNHAM PREBYS MEDICAL DISCOVERY INSTITUTE	10901 N TORREY PINES RD	LA JOLLA	CA	92037	SAN DIEGO	USA	Arthritis, Musculoskeletal and Skin Diseases Research	000	4	5/30/2024	NON-COMPETING CONTINUATION	$676,107
														Subtotal = $676,107

Issue Date FY: 2023 ( Subtotal = $607,182 )
2023	2023	SANFORD BURNHAM PREBYS MEDICAL DISCOVERY INSTITUTE	10901 N TORREY PINES RD	LA JOLLA	CA	92037	SAN DIEGO	USA	Arthritis, Musculoskeletal and Skin Diseases Research	000	3	5/22/2023	NON-COMPETING CONTINUATION	$607,182
														Subtotal = $607,182

Issue Date FY: 2022 ( Subtotal = $599,047 )
2022	2022	SANFORD BURNHAM PREBYS MEDICAL DISCOVERY INSTITUTE	10901 NORTH TORREY PINES RD	LA JOLLA	CA	92037	SAN DIEGO	USA	Arthritis, Musculoskeletal and Skin Diseases Research	000	2	5/10/2022	NON-COMPETING CONTINUATION	$599,047
														Subtotal = $599,047

Issue Date FY: 2021 ( Subtotal = $600,533 )
2021	2021	SANFORD BURNHAM PREBYS MEDICAL DISCOVERY INSTITUTE	10901 NORTH TORREY PINES RD	LA JOLLA	CA	92037	SAN DIEGO	USA	Arthritis, Musculoskeletal and Skin Diseases Research	000	1	8/4/2021	NEW	$600,533
														Subtotal = $600,533

Grand Total All Awards = $2,482,869

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Mechanisms that couple irregular development of fetal melanoblasts to premature exhaustion of adult melanocyte stem cells

Award Number: R01AR078559

ORGANIZATION: NATIONAL INSTITUTE OF ARTHRITIS & MUSCULOSKELETAL & SKIN DISEASES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

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