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PhAIge Therapy Center: AI-Guided Tailspike Engineering and Molecular Typing for Rapid Phage Design and Testing Against ESKAPE Pathogens

Award Number: P01AI195327
ORGANIZATION: NATIONAL INSTITUTE OF ALLERGY & INFECTIOUS DISEASES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 05/11/2026
PERIOD OF PERFORMANCE END DATE: 04/30/2031

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PhAIge Therapy Center: AI-Guided Tailspike Engineering and Molecular Typing for Rapid Phage Design and Testing Against ESKAPE Pathogens - PROJECT SUMMARY/ABSTRACT The Gladstone PhAIge Therapy Center seeks to address the critical threat of multidrug-resistant ESKAPE pathogens by developing next-generation preclinical assays and computational tools. Our overarching goal is to establish a robust, quantitative, and predictive preclinical framework that accelerates the pipeline from phage discovery to clinical application, with a primary focus on Klebsiella pneumoniae. Specifically, Project 1 develops high-throughput, sequencing-based assays to measure rate constants for each component of the phage infection cycle (recognition, entry, replication, packaging, and lysis). By capturing granular, quantitative data, this work will enable the rational selection and engineering of phages that exhibit superior efficacy. Project 2 employs multiplexed assays to characterize K. pneumoniae strains rapidly by their capsule types and susceptibility profiles. Integrating capsule-typing with systematic phage compatibility testing will allow for the creation of optimized, off-the-shelf phage cocktails tailored to individual clinical isolates. The Center’s strategy includes two specialized Cores to ensure translational relevance and efficiency. The Advanced Computational Core combines bioinformatics pipelines, AI-driven modeling, and machine learning to predict phage-host interactions and guide phage selection. The Organoid and Human Cell Culture Core incorporates 3D human tissue models and primary cell cultures into early screening, capturing host-pathogen dynamics that are overlooked by standard in vitro assays. An Administrative Core fosters collaboration, manages the Development and Research Program (DRP) to support innovative pilot projects, and liaises closely with clinical and industry advisors. By uniting modern molecular methods, computational analytics, and human- relevant models, the Gladstone PhAIge Therapy Center will advance systematic, clinically ready phage therapies to combat antibiotic resistance.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2026 ( Subtotal = $2,047,959 )
2026	2026	J.DAVID GLADSTONE INSTITUTES	1650 OWENS ST	SAN FRANCISCO	CA	94158	SAN FRANCISCO	USA	Allergy and Infectious Diseases Research	000	1	5/12/2026	NEW	$2,047,959
														Subtotal = $2,047,959

Grand Total All Awards = $2,047,959

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PhAIge Therapy Center: AI-Guided Tailspike Engineering and Molecular Typing for Rapid Phage Design and Testing Against ESKAPE Pathogens

Award Number: P01AI195327

ORGANIZATION: NATIONAL INSTITUTE OF ALLERGY & INFECTIOUS DISEASES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 05/11/2026

PERIOD OF PERFORMANCE END DATE: 04/30/2031

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