Official websites use .gov

A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS

A lock ( 🔒 ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

Need Help

Long-Range Regulatory Interactions in the Drosophila Brain.

Award Number: K99NS138574
ORGANIZATION: NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: TRAINING/TRAINEESHIP
PERIOD OF PERFORMANCE START DATE: 05/01/2025
PERIOD OF PERFORMANCE END DATE: 04/30/2027

Group Awards By:

View Award Description

Long-Range Regulatory Interactions in the Drosophila Brain. - This research proposal is dedicated to unraveling the intricate regulatory mechanisms governing chromatin organization and gene transcription during neural development in the model organism Drosophila. The primary focus is on understanding how 3D genome architecture, specifically chromatin loops, contributes to the spatial and temporal control of gene expression, particularly in the context of cell adhesion molecule (CAM) genes. This comprehensive investigation involves a multi-faceted approach, combining cutting-edge experimental techniques, advanced computational analyses, and genetic manipulations. The first aim involves the identification of focal contact anchors using Micro-C maps, high-resolution chromatin contacts maps. These maps will be generated for various developmental stages, including the wing imaginal disc, larval brain, and adult brain. The analysis will also include CAM genes, a significant group of genes with critical roles in neural development. This aim will provide insight into the specific chromatin regions involved in gene regulation and spatial organization. The second aim delves into the functional role of identified focal contact anchors in regulating gene transcription. By employing precise deletions and insertions of these anchors, the study aims to decipher their contribution to gene expression. The investigation will focus on CAM genes, aiming to distinguish between essential anchors that disrupt transcription upon deletion and assisting anchors that do not significantly impact gene expression. This analysis will provide a nuanced understanding of the functional significance of these anchors in gene regulation. The third aim focuses on dissecting the involvement of transcription factors in the formation of chromatin loops. Through motif enrichment analysis and depletion experiments using RNA interference, the study aims to identify critical transcription factors that contribute to loop formation. This aim will provide insights into the intricate interplay between transcription factors and chromatin architecture, shedding light on the complex regulatory mechanisms governing gene expression. The proposed research holds potential for transformative contributions to our understanding of neural development and gene regulation. By dissecting the role of 3D genome organization in gene expression, this study bridges the gap between chromatin architecture and functional gene networks. This research not only contributes to fundamental insights into neural circuit assembly but also has broader implications for our understanding of gene regulation in diverse biological contexts.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2026 ( Subtotal = $127,145 )
2026	2026	THE TRUSTEES OF PRINCETON UNIVERSITY	1 NASSAU HALL	PRINCETON	NJ	08544	MERCER	USA	Extramural Research Programs in the Neurosciences and Neurological Disorders	000	2	6/23/2026	NON-COMPETING CONTINUATION	$127,145
														Subtotal = $127,145

Issue Date FY: 2025 ( Subtotal = $127,145 )
2025	2025	THE TRUSTEES OF PRINCETON UNIVERSITY	1 NASSAU HALL	PRINCETON	NJ	08544	MERCER	USA	Extramural Research Programs in the Neurosciences and Neurological Disorders	000	1	4/22/2025	NEW	$127,145
														Subtotal = $127,145

Grand Total All Awards = $254,290

Top

All Categories

About

Search

Reports

Data Submission

Award Information

Long-Range Regulatory Interactions in the Drosophila Brain.

Award Number: K99NS138574

ORGANIZATION: NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: TRAINING/TRAINEESHIP

PERIOD OF PERFORMANCE START DATE: 05/01/2025

PERIOD OF PERFORMANCE END DATE: 04/30/2027

Federal Websites

Department of Health & Human Services

HHS Operating Divisions

HHS Staff Divisions

Download A Document Viewer