Official websites use .gov

A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS

A lock ( 🔒 ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

Need Help

The Molecular Basis of Transcription-Replication Conflicts and Pathways to Resolution

Award Number: K99GM163010
ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: TRAINING/TRAINEESHIP
PERIOD OF PERFORMANCE START DATE: 03/01/2026
PERIOD OF PERFORMANCE END DATE: 02/29/2028

Group Awards By:

View Award Description

The Molecular Basis of Transcription-Replication Conflicts and Pathways to Resolution - Project Summary/Abstract: Transcription and replication machineries frequently collide, leading to transcription-replication conflicts that destabilize the genome if not resolved, which contributes to diseases such as cancer. Despite their destructive potential, the mechanisms behind these conflicts and their resolution are poorly understood. This proposal aims to identify novel factors involved in resolving these conflicts, define how they form, and provide mechanistic insights into their resolution. By combining computational predictions, advanced biochemistry, and cryo-EM, this research will reveal how genomic stability is maintained in the face of these conflicts. During the beginning of my postdoc, I investigated how genome stability is maintained through the recruitment of key factors, demonstrating that the transcription machinery directly promotes the deposition of a histone modification that recruits stability-maintaining factors. I am now focused on understanding transcription-replication conflicts, a critical source of genome instability. I have already purified the necessary components for this project and visualized interactions between transcription machinery and stability-promoting factors, as well as replication machinery with a genome-stabilizing factor. In Aim 1, I will use AlphaFold predictions to identify novel factors recruited to transcription-replication conflicts and employ cryo-EM to visualize their interactions with the transcription or replication machinery. Aim 2 will reconstitute transcription-replication conflicts in vitro, enabling manipulation and visualization of these reactions to understand how various factors contribute. In Aim 3, I will investigate how the Integrator Complex works with replication machinery to resolve conflicts. During the K99 phase, I will develop systems for reconstituting and manipulating transcription- replication conflicts under expert mentorship. This training will provide the skills necessary for independent research in the R00 phase, where I will define the molecular mechanisms of conflict resolution. My research aligns with the NIH’s mission to advance biomedical knowledge and improve human health. By uncovering mechanisms behind transcription-replication conflicts, my work will enhance our understanding of genome stability and contribute to preventing diseases such as cancer. My environment offers cutting-edge technology, expert mentorship, and a collaborative community, positioning me to make meaningful contributions to the field and establish myself as a successful, independent researcher.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2026 ( Subtotal = $125,000 )
2026	2026	PRESIDENT AND FELLOWS OF HARVARD COLLEGE	25 SHATTUCK ST	BOSTON	MA	02115	SUFFOLK	USA	Biomedical Research and Research Training	000	1	2/9/2026	NEW	$125,000
														Subtotal = $125,000

Grand Total All Awards = $125,000

Top

All Categories

About

Search

Reports

Data Submission

Award Information

The Molecular Basis of Transcription-Replication Conflicts and Pathways to Resolution

Award Number: K99GM163010

ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: TRAINING/TRAINEESHIP

PERIOD OF PERFORMANCE START DATE: 03/01/2026

PERIOD OF PERFORMANCE END DATE: 02/29/2028

Federal Websites

Department of Health & Human Services

HHS Operating Divisions

HHS Staff Divisions

Download A Document Viewer