Official websites use .gov

A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS

A lock ( 🔒 ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

Need Help

Mechanisms and accurate diagnosis of cefazolin allergy

Award Number: K23AI190126
ORGANIZATION: NATIONAL INSTITUTE OF ALLERGY & INFECTIOUS DISEASES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: TRAINING/TRAINEESHIP
PERIOD OF PERFORMANCE START DATE: 05/06/2026
PERIOD OF PERFORMANCE END DATE: 04/30/2031

Group Awards By:

View Award Description

Mechanisms and accurate diagnosis of cefazolin allergy - PROJECT SUMMARY/ABSTRACT This K23 award application describes a career development plan for Dr. Alexei Gonzalez-Estrada and is designed to provide the training and support needed to facilitate his becoming an independent clinician- investigator specializing in perioperative anaphylaxis and drug allergy. The candidate’s career goal is to become a leader in drug allergy by integrating clinical practice and research focusing on mechanisms, diagnostics, and prevention. Specifically, this proposal will enable Dr. Gonzalez-Estrada to accomplish the following: (1) Acquire hands-on experience by developing drug allergy mouse models and dissecting the mechanisms, (2) Advance his in vitro drug allergy testing skills by acquiring a cell-based assay, (3) Enhance his clinical and translational research training by taking courses in the Mayo Center for Clinical and Translational Science, and (4) Enhance his communication and leadership skills. The findings will serve as preliminary data to apply for R01s and other funding opportunities. The prevalence of cephalosporin allergy is 0.5-2%, making it one of the most common drug classes causing immediate drug allergy. Cefazolin, a frequently used cephalosporin, is the most common cause of perioperative anaphylaxis – a potentially fatal immediate drug allergy. However, the immunologic mechanisms of cefazolin allergy are not well understood, and current clinical tests for cefazolin allergy have several limitations. To fill the gaps, the specific goals of the research proposal are to elucidate the immunologic mechanisms by which cefazolin induces anaphylaxis, identify the antigenic epitopes of cefazolin allergy, and evaluate the clinical performance of a new in vitro cefazolin allergy test. We will use a novel cefazolin allergy mouse model that manifests clinical symptoms of anaphylaxis and peptide microarray platform combined with machine learning to identify cefazolin allergy mimotopes. To evaluate the performance of a cell-based in vitro test, we will perform a prospective multicenter clinical study. Dr. Gonzalez-Estrada will complete a basic science internship and external internships, including mouse models, peptide microarray technology, and cell-based in vitro testing. In addition, he will complete formal didactic coursework in clinical research study design, biostatistics, and bioethics among others. Dr. Gonzalez-Estrada has assembled a mentoring committee comprised of international experts in perioperative anaphylaxis and drug allergy, type 2 immunity, and protein biochemistry uniquely suited to address the hypotheses set forth in this proposal and guide Dr. Gonzalez-Estrada through this stage of his career development. This proposal combines the strength of the research environment at Mayo Clinic and an ongoing collaboration with the University of Antwerp and Arizona State University to pursue the training/research goals. At the conclusion of this program, Dr. Gonzalez-Estrada will have the skillset and experience required to transition to independence and lead a translational and clinical research team.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2026 ( Subtotal = $201,636 )
2026	2026	MAYO CLINIC ARIZONA	13400 E SHEA BLVD	SCOTTSDALE	AZ	85259	MARICOPA	USA	Allergy and Infectious Diseases Research	000	1	5/7/2026	NEW	$201,636
														Subtotal = $201,636

Grand Total All Awards = $201,636

Top

All Categories

About

Search

Reports

Data Submission

Award Information

Mechanisms and accurate diagnosis of cefazolin allergy

Award Number: K23AI190126

ORGANIZATION: NATIONAL INSTITUTE OF ALLERGY & INFECTIOUS DISEASES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: TRAINING/TRAINEESHIP

PERIOD OF PERFORMANCE START DATE: 05/06/2026

PERIOD OF PERFORMANCE END DATE: 04/30/2031

Federal Websites

Department of Health & Human Services

HHS Operating Divisions

HHS Staff Divisions

Download A Document Viewer