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Machine learning-based methods for the analysis of microbial glycomes and proteomes in inflammatory bowel disease.

Award Number: K08DK132516
ORGANIZATION: NATIONAL INSTITUTE OF DIABETES & DIGESTIVE & KIDNEY DISEASES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: TRAINING/TRAINEESHIP
PERIOD OF PERFORMANCE START DATE: 01/04/2023
PERIOD OF PERFORMANCE END DATE: 11/30/2027

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Machine learning-based methods for the analysis of microbial glycomes and proteomes in inflammatory bowel disease. - Inflammatory bowel disease (IBD) affects over 1.2 million patients in the United States and causes significant morbidity and healthcare expenditures. Studies have associated the development of IBD with changes in the human gut microbiome, which together with genetic and environmental factors alter immune responses to gut flora and cause chronic inflammation. The surface and secreted proteins and glycans of gut microbes mediates many aspects of these immune interactions. However, the study of these molecules is limited by the extreme complexity of the gut environment. Standard proteomic techniques only capture a small fraction of the predicted microbial gene products while metagenomic analyses using automated annotations fail to identify functions for nearly half of all predicted proteins. The dietary, host, and microbial contributions to the diverse carbohydrate pool also makes the analysis of microbial glycans in stool samples highly challenging. The incomplete evaluation of microbial surface and secreted proteins and microbial glycans impedes the discovery of new biological insights into IBD. Machine learning algorithms, and especially advancements in natural language processing (NLP) based on deep neural networks, have enabled major improvements in the accuracy of a number of tasks related to human speech and written text. These deep neural networks function by analyzing massive collections of texts and then creating high-dimensional vectors to represent the semantic meaning of words without the need for specific labels. Biological polymers such as DNA, proteins, and glycans are also long complex sequences, and application of NLP techniques enabled accurate predictions of the functional and structural characteristics of proteins and glycans from primary sequence. We hypothesize that machine learning methods incorporating deep neural networks can be successfully applied to the analysis of microbial metagenomes and glycomes to identify previously unknown perturbations in IBD. We will test this hypothesis with the following aims: 1) Develop and adapt deep learning algorithms to analyze the surface-associated and secreted gut microbial metaproteome in IBD; 2) Create and apply deep learning algorithms to analyze the fecal microbial glycome in IBD; 3) Experimentally validate the functions of a subset of novel microbial proteins and glycans that are altered in IBD. The long-term goal of this project is to discover new biological insights into the pathogenesis, progression, and treatment of IBD. This proposal comprises a five-year research career development program focused on the creation and adaptation of deep learning algorithms to the analysis of gut microbial metagenomic and glycomic data. The candidate is an Instructor of Medicine at Harvard Medical School and the Division of Gastroenterology at Massachusetts General Hospital. He has assembled an outstanding group of collaborators and advisors with deep expertise in machine learning, glycobiology, microbiome analysis, and IBD. Under the guidance of his mentors Dr. James Collins and Dr. Tristan Bepler, the proposed experiments and training will equip the candidate with a unique set of skills that will enable him to transition to independence as a physician-scientist.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2026 ( Subtotal = $169,560 )
2026	2026	THE GENERAL HOSPITAL CORPORATION	55 FRUIT ST	BOSTON	MA	02114	SUFFOLK	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	000	4	12/18/2025	NON-COMPETING CONTINUATION	$169,560
														Subtotal = $169,560

Issue Date FY: 2025 ( Subtotal = $169,560 )
2025	2025	THE GENERAL HOSPITAL CORPORATION	55 FRUIT ST	BOSTON	MA	02114	SUFFOLK	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	000	3	11/20/2024	NON-COMPETING CONTINUATION	$169,560
														Subtotal = $169,560

Issue Date FY: 2024 ( Subtotal = $169,560 )
2024	2024	THE GENERAL HOSPITAL CORPORATION	55 FRUIT ST	BOSTON	MA	02114	SUFFOLK	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	000	2	11/7/2023	NON-COMPETING CONTINUATION	$169,560
														Subtotal = $169,560

Issue Date FY: 2023 ( Subtotal = $169,560 )
2023	2023	MASSACHUSETTS GENERAL HOSPITAL, THE	55 FRUIT ST	BOSTON	MA	02114	SUFFOLK	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	000	1	1/4/2023	NEW	$169,560
														Subtotal = $169,560

Grand Total All Awards = $678,240

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Machine learning-based methods for the analysis of microbial glycomes and proteomes in inflammatory bowel disease.

Award Number: K08DK132516

ORGANIZATION: NATIONAL INSTITUTE OF DIABETES & DIGESTIVE & KIDNEY DISEASES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: TRAINING/TRAINEESHIP

PERIOD OF PERFORMANCE START DATE: 01/04/2023

PERIOD OF PERFORMANCE END DATE: 11/30/2027

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