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Spatiotemporal Regulation of AMPK During Amoeboid Migration

Award Number: F32GM167153
ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: FELLOWSHIP/SCHOLARSHIP/STUDENT LOANS
PERIOD OF PERFORMANCE START DATE: 08/01/2026
PERIOD OF PERFORMANCE END DATE: 07/31/2028

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Spatiotemporal Regulation of AMPK During Amoeboid Migration - Abstract Cell migration is fundamental to development, immunity, and disease, yet the molecular mechanisms governing directed amoeboid migration in vivo remain poorly understood. AMP-activated protein kinase (AMPK), canonically known as a homeostatic energy sensor, has emerged as a critical regulator of cell polarity and actomyosin contractility during migration. However, the spatiotemporal regulation of AMPK in migrating cells and how it integrates metabolic cues with directional movement are unclear. This research proposes to use Drosophila primordial germ cells (PGCs) as a genetically tractable in vivo model to investigate how AMPK coordinates cellular metabolism with contractile machinery during amoeboid migration. Building on preliminary data showing AMPK enrichment on endolysosomes in isolated PGCs and early evidence of CaMKK2- dependent migration defects, this proposal employs three integrated aims: (1) determine the spatial localization mechanisms of AMPK on endolysosomes and test whether this localization is functionally required for directed migration, (2) characterize AMPK activity dynamics using live biosensors and define the role of calcium- CaMKK2 signaling in AMPK activation, and (3) assess whether ATP depletion contributes to spatial AMPK regulation during migration. This work will provide the first spatiotemporally-resolved view of AMPK function in migrating cells in vivo and establish a mechanistic framework for how cells couple metabolism with contractility. The findings have broad implications for understanding pathological migration in cancer metastasis and immune cell trafficking, with potential therapeutic applications.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2026 ( Subtotal = $79,348 )
2026	2026	THE RESEARCH FOUNDATION FOR THE STATE UNIVERSITY OF NEW YORK	W5510 FRANKS MELVILLE MEMORIAL LIBRARY	STONY BROOK	NY	11794	SUFFOLK	USA	Biomedical Research and Research Training	001	1	7/22/2026	NEW	$0
2026	2026	THE RESEARCH FOUNDATION FOR THE STATE UNIVERSITY OF NEW YORK	W5510 FRANKS MELVILLE MEMORIAL LIBRARY	STONY BROOK	NY	11794	SUFFOLK	USA	Biomedical Research and Research Training	000	1	7/22/2026	NEW	$79,348
														Subtotal = $79,348

Grand Total All Awards = $79,348

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Spatiotemporal Regulation of AMPK During Amoeboid Migration

Award Number: F32GM167153

ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: FELLOWSHIP/SCHOLARSHIP/STUDENT LOANS

PERIOD OF PERFORMANCE START DATE: 08/01/2026

PERIOD OF PERFORMANCE END DATE: 07/31/2028

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