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Discovering signature genes and developing expression markers for blastema cells

Award Number: F32GM117806
ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: FELLOWSHIP/SCHOLARSHIP/STUDENT LOANS
PERIOD OF PERFORMANCE START DATE: 04/25/2016
PERIOD OF PERFORMANCE END DATE: 03/03/2019

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Discovering signature genes and developing expression markers for blastema cells - DESCRIPTION (provided by applicant): The objective of this three-year postdoctoral training program is to empower the applicant with the necessary skills and network to establish an independent laboratory in the United States studying regeneration in newts (salamanders). Newts are critical components of the regenerative research landscape due to their ability to replace complex body parts after loss. Limb regeneration is an important model in this regard. Central to newt limb regeneration is the formation of a blastema; a heterogeneous mass of cells that develop into the new limb. Despite its centrality, we have a minimal understanding of the blastema and lack any specific expression markers. The applicant will identify the genes and cis-regulatory elements that regulate blastema formation in the Iberian newt and develop fluorescent expression markers to identify blastema cell types in vivo. This will be accomplished through the following research plan. First, functionally relevant genomic regions will be sequenced and assembled by a combination of whole genome sequencing and selectively sequencing open chromatin (Aim 1). Second, using single-cell transcriptomics, the cell types contributing to the blastema at will be classified and marker genes identified. Additionally, by integrating this single-cell transcriptomic information with obtained genomic sequences, cis-regulatory elements that are over- or under- represented in blastema markers genes will be identified (Aim 2). Third, using CRISPR/Cas9 genome editing, transgenic newts expressing fluorescent blastema markers will be developed (Aim 3). The identified genes, cis-regulatory elements and transgenic newts will offer unprecedented knowledge and tools to further understand the regulation of regeneration, thereby enabling the applicant to begin a promising independent career in regeneration research.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2024 ( Subtotal = -$7,723 )
2024	2018	REDACTED DUE TO PII		SOLNA				SWE	Biomedical Research and Research Training	000	3	10/1/2023	NON-COMPETING CONTINUATION	-$7,723
														Subtotal = -$7,723

Issue Date FY: 2019 ( Subtotal = -$7,723 )
2019	2018	KAROLINSKA INST	SOLNAVAGEN 1	SOLNA		17165		SWE	Biomedical Research and Research Training	001	3	8/23/2019	NON-COMPETING CONTINUATION	$0
2019	2018	REDACTED DUE TO PII		SOLNA		17165		SWE	Biomedical Research and Research Training	000	3	8/20/2019	NON-COMPETING CONTINUATION	-$7,723
														Subtotal = -$7,723

Issue Date FY: 2018 ( Subtotal = $9,850 )
2018	2018	KAROLINSKA INST	SOLNAVAGEN 1	SOLNA		17165		SWE	Biomedical Research and Research Training	001	3	4/24/2018	NON-COMPETING CONTINUATION	$8,850
2018	2018	KAROLINSKA INST	SOLNAVAGEN 1	SOLNA		17165		SWE	Biomedical Research and Research Training	003	3	5/16/2018	NON-COMPETING CONTINUATION	$1,000
														Subtotal = $9,850

Issue Date FY: 2017 ( Subtotal = $8,850 )
2017	2017	KAROLINSKA INST	NOBELS VAG 5	SOLNA		17165		SWE	Biomedical Research and Research Training	001	2	3/22/2017	NON-COMPETING CONTINUATION	$8,850
														Subtotal = $8,850

Issue Date FY: 2016 ( Subtotal = $8,850 )
2016	2016	KAROLINSKA INSTITUTE, RYLCAR INST OF MEDICINE & SURGERY	DEPT OF NEUROSCIENCE S-171 77	STOCKHOLM				SWE	Biomedical Research and Research Training	003	1	3/18/2016	NEW	$8,850
2016	2016	KAROLINSKA INSTITUTE, RYLCAR INST OF MEDICINE & SURGERY	DEPT OF NEUROSCIENCE S-171 77	STOCKHOLM				SWE	Biomedical Research and Research Training	002	1	3/18/2016	NEW	$0
														Subtotal = $8,850

Grand Total All Awards = $12,104

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Discovering signature genes and developing expression markers for blastema cells

Award Number: F32GM117806

ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: FELLOWSHIP/SCHOLARSHIP/STUDENT LOANS

PERIOD OF PERFORMANCE START DATE: 04/25/2016

PERIOD OF PERFORMANCE END DATE: 03/03/2019

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