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THE ORIGIN AND ROLE OF KINETOCHORE FIBERS DURING MITOSIS

Award Number: F32GM077911
ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: FELLOWSHIP/SCHOLARSHIP/STUDENT LOANS
PERIOD OF PERFORMANCE START DATE: 06/12/2006
PERIOD OF PERFORMANCE END DATE: 06/11/2009

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THE ORIGIN AND ROLE OF KINETOCHORE FIBERS DURING MITOSIS - DESCRIPTION (provided by applicant): The Khodjakov lab has recently shown that formation of K-fibers during mitosis can occur via mechanisms intrinsic to the kinetochores, while centrosomes are responsible for the orientation of K-fibers toward spindle poles. Molecular mechanisms responsible for the kinetochore-mediated K-fiber formation remain unknown. We propose is to test the hypothesis that initiation of K-fiber formation involves capture of short microtubules that form in the vicinity of the kinetochore via centrosome-independent mechanisms. To test this hypothesis, we will laser-ablate centrosomes to remove astral microtubules, and simultaneously eliminate interzonal microtubules by siRNA of PRC1, a protein required for formation of the midzone. This approach will allow us to follow formation of K-fibers (labeled via a-tubulin/GFP expression) by time-lapse microscopy with unprecedented clarity and precision. The origin of microtubules comprising K-fibers and their behavior during K-fiber formation will be determined by EM tomography of cells fixed at various stages of the process. In addition to this primary goal, our experiments will reveal the extent to which centrosome-independent mechanisms operate by generating mitotic spindles comprised only of K-fibers.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2008 ( Subtotal = $48,796 )
2008	2008	HEALTH RESEARCH, INC - ALBANY DIVISION	1 UNIVERSITY PLACE	RENSSELAER	NY	12144	RENSSELAER	USA	Biomedical Research and Research Training	000	3	5/27/2008	NON-COMPETING CONTINUATION	$48,796
														Subtotal = $48,796

Issue Date FY: 2007 ( Subtotal = $45,976 )
2007	2007	HEALTH RESEARCH, INC - ALBANY DIVISION	1 UNIVERSITY PLACE	RENSSELAER	NY	12144	RENSSELAER	USA	Biomedical Research and Research Training	000	2	6/19/2007	NON-COMPETING CONTINUATION	$45,976
														Subtotal = $45,976

Issue Date FY: 2006 ( Subtotal = $43,996 )
2006	2006	HEALTH RESEARCH, INC - ALBANY DIVISION	1 UNIVERSITY PLACE	RENSSELAER	NY	12144	RENSSELAER	USA	Biomedical Research and Research Training	000	1	3/31/2006	NEW	$43,996
														Subtotal = $43,996

Grand Total All Awards = $138,768

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THE ORIGIN AND ROLE OF KINETOCHORE FIBERS DURING MITOSIS

Award Number: F32GM077911

ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: FELLOWSHIP/SCHOLARSHIP/STUDENT LOANS

PERIOD OF PERFORMANCE START DATE: 06/12/2006

PERIOD OF PERFORMANCE END DATE: 06/11/2009

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