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Identifying the Mechanisms and Localization of Activity-Dependent CaMKII Synthesis

Award Number: F31NS134189
ORGANIZATION: NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: FELLOWSHIP/SCHOLARSHIP/STUDENT LOANS
PERIOD OF PERFORMANCE START DATE: 07/16/2023
PERIOD OF PERFORMANCE END DATE: 07/15/2026

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Identifying the Mechanisms and Localization of Activity-Dependent CaMKII Synthesis - Project Summary At the Drosophila neuromuscular junction (NMJ), repetitive stimulation has been shown to induce various forms of protein synthesis-dependent plasticity, including growth of immature “ghost” boutons and potentiation of miniature excitatory junction potential (mEJP) frequency. There is a concurrent increase in calcium/calmodulin-dependent protein kinase II (CaMKII) protein abundance, which is required for plasticity. The molecular mechanisms of this increase in CaMKII are not well understood, and previous studies examining this effect did not differentiate between the presynaptic and postsynaptic sides of the NMJ. The Drosophila NMJ has a wide variety of genetic tools to separate the motoneuron and muscle, making it an ideal model for separating presynaptic and postsynaptic mechanisms. Preliminary data indicate that after spaced depolarization with a high potassium solution, CaMKII levels increase in both the presynaptic and postsynaptic sides of the NMJ. Additionally, this increase was blocked by application of cycloheximide, a translation inhibitor, indicating that CaMKII accumulation is dependent on protein synthesis and is unlikely to be due to protein translocation or transport. The goal of this research proposal is to identify the molecular mechanisms and localization of CaMKII synthesis on both sides of the synapse. In the first part of Aim 1, GFP reporter constructs will be used to determine the sequences in the CaMKII 3’UTR that are required for activity- dependent translation. In the second part of Aim 1, the trans-acting proteins that bind to the UTR will be identified using RNA affinity purification. The results from these experiments will elucidate the specific cis- and trans-acting factors that are required for activity-dependent CaMKII protein synthesis, and determine whether similar mechanisms act pre- and post-synaptically. In Aim 2, Flp-Frt recombination will be used to delete either the CaMKII catalytic domain or the 3’UTR and the effect on potentiation of mEJP frequency will be determined. The excision of the domains will be done exclusively in the presynaptic or postsynaptic compartment, so that the contribution of either the motoneuron or muscle can be clearly separated. In Aim 3, the subcellular localization of newly-synthesized CaMKII will be compared to that of pre-existing CaMKII. The function of this newly-synthesized CaMKII pool is unknown, and this experiment will determine whether the newly-synthesized CaMKII is targeted to a distinct subcellular nanodomain. Ultimately, this research plan will clarify how compartment-specific CaMKII synthesis may be contributing to long-term functional changes in the synapse and may identify new avenues of research into CaMKII-dependent plasticity. While conducting this research, in-depth training will be provided in a variety of crucial skills, including research design, statistical analysis, communication skills, super-resolution microscopy, and image analysis.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2025 ( Subtotal = $38,887 )
2025	2025	BRANDEIS UNIVERSITY	415 SOUTH ST	WALTHAM	MA	02453	MIDDLESEX	USA	Extramural Research Programs in the Neurosciences and Neurological Disorders	000	3	5/26/2025	NON-COMPETING CONTINUATION	$38,887
														Subtotal = $38,887

Issue Date FY: 2024 ( Subtotal = $38,233 )
2024	2024	BRANDEIS UNIVERSITY	415 SOUTH ST	WALTHAM	MA	02453	MIDDLESEX	USA	Extramural Research Programs in the Neurosciences and Neurological Disorders	000	2	6/13/2024	NON-COMPETING CONTINUATION	$38,233
														Subtotal = $38,233

Issue Date FY: 2023 ( Subtotal = $38,631 )
2023	2023	BRANDEIS UNIVERSITY	415 SOUTH ST	WALTHAM	MA	02453	MIDDLESEX	USA	Extramural Research Programs in the Neurosciences and Neurological Disorders	001	1	7/12/2023	NEW	$0
2023	2023	BRANDEIS UNIVERSITY	415 SOUTH ST	WALTHAM	MA	02453	MIDDLESEX	USA	Extramural Research Programs in the Neurosciences and Neurological Disorders	000	1	7/10/2023	NEW	$38,631
														Subtotal = $38,631

Grand Total All Awards = $115,751

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Identifying the Mechanisms and Localization of Activity-Dependent CaMKII Synthesis

Award Number: F31NS134189

ORGANIZATION: NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: FELLOWSHIP/SCHOLARSHIP/STUDENT LOANS

PERIOD OF PERFORMANCE START DATE: 07/16/2023

PERIOD OF PERFORMANCE END DATE: 07/15/2026

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