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MECHANISM FOR LOADING THE E. COLI BETA CLAMP ON DNA

Award Number: F31GM073586
ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: FELLOWSHIP/SCHOLARSHIP/STUDENT LOANS
PERIOD OF PERFORMANCE START DATE: 04/02/2005
PERIOD OF PERFORMANCE END DATE: 09/01/2008

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MECHANISM FOR LOADING THE E. COLI BETA CLAMP ON DNA - DESCRIPTION (provided by applicant): The long-term goal of this research is to understand how organisms coordinate the replication and repair of their genomes. The use of processivity clamps that must be loaded onto DNA during replication of chromosomes by an ATP dependent clamp loader complex is highly conserved by all forms of life. Understanding how organisms coordinate DNA replication and repair is the fundamental basis for understanding human diseases such as cancer. The goal of the work proposed here is to better define the mechanism of clamp loading in the E. coli model system. The E. coli clamp loader (gamma complex) is a multisubunit complex that undergoes a conformational change upon binding ATP which facilitates opening of the beta clamp and its subsequent loading onto DNA. The contacts of the individual clamp loader subunits with beta differ between the ATP bound and free states of gamma complex. The interactions of the gamma complex subunits with beta in both the presence and absence of ATP will be determined. Biochemical assays using various clamp and clamp loader mutants will be utilized to accomplish this. The insight gained from E. coli can provide a framework for elucidating the mechanisms of eukaryotes, including humans.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2008 ( Subtotal = $8,655 )
2008	2008	RESEARCH FOUNDATION OF STATE UNIV OF NEW YORK (SUNY)	PO BOX 9	ALBANY	NY	12201	ALBANY	USA	Biomedical Research and Research Training	001	3	5/12/2008	SUPPLEMENT FOR EXPANSION	$8,655
														Subtotal = $8,655

Issue Date FY: 2007 ( Subtotal = $25,448 )
2007	2007	RESEARCH FOUNDATION OF STATE UNIV OF NEW YORK (SUNY)	PO BOX 9	ALBANY	NY	12201	ALBANY	USA	Biomedical Research and Research Training	000	3	3/30/2007	NON-COMPETING CONTINUATION	$25,448
														Subtotal = $25,448

Issue Date FY: 2006 ( Subtotal = $25,448 )
2006	2006	RESEARCH FOUNDATION OF STATE UNIV OF NEW YORK (SUNY)	PO BOX 9	ALBANY	NY	12201	ALBANY	USA	Biomedical Research and Research Training	000	2	3/30/2006	NON-COMPETING CONTINUATION	$25,448
														Subtotal = $25,448

Issue Date FY: 2005 ( Subtotal = $25,448 )
2005	2005	RESEARCH FOUNDATION OF STATE UNIV OF NEW YORK (SUNY)	PO BOX 9	ALBANY	NY	12201	ALBANY	USA	Biomedical Research and Research Training	000	1	3/28/2005	NEW	$25,448
														Subtotal = $25,448

Grand Total All Awards = $84,999

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MECHANISM FOR LOADING THE E. COLI BETA CLAMP ON DNA

Award Number: F31GM073586

ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: FELLOWSHIP/SCHOLARSHIP/STUDENT LOANS

PERIOD OF PERFORMANCE START DATE: 04/02/2005

PERIOD OF PERFORMANCE END DATE: 09/01/2008

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