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Determining the cellular and nanoscale localization of Synaptotagmin VII

Award Number: F30NS130973
ORGANIZATION: NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: FELLOWSHIP/SCHOLARSHIP/STUDENT LOANS
PERIOD OF PERFORMANCE START DATE: 12/05/2022
PERIOD OF PERFORMANCE END DATE: 07/31/2026

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Determining the cellular and nanoscale localization of Synaptotagmin VII - ABSTRACT Synaptic dysfunction is linked to numerous devastating neurological and psychiatric disease. Synaptic transmission is initiated by the calcium dependent release of neurotransmitters. The calcium sensors responsible for triggering vesicle release are the Synaptotagmins (SYT), a 17 protein family. The function of one SYT, SYT7, is less well understood but is thought to be of significant importance due to its broad expression throughout the body and brain, connections to cancer and ALS, and enigmatic function in the central nervous system. Unlike other Synaptotagmins, SYT7 is thought to be found on the plasma membrane and its function remains controversial. The role of SYT7 is still under intense debate due to several outstanding questions regarding its cellular and synaptic localization. Answering these key questions is the focus of my grant proposal and will provide necessary insights into SYT7’s function. This proposal aims to answer these questions by determining SYT7’s cellular and nanoscale localization in cortical neurons using biochemistry and three-color STED super resolution microscopy. Aim 1 will determine whether SYT7 is found on the cell surface of cortical neurons using live-cell surface protein biotinylation and live-cell surface staining in mouse cortical slices and rat primary cortical neurons. Aim 2 will determine how SYT7 is organized at synapses using three-color STED staining for endogenous synaptic proteins. Aim 3 will determine whether neuronal activity regulates the cellular and nanoscale localization of SYT7 using both surface protein biotinylation and live-cell surface staining with three-color STED. This proposal is designed to provide me with intellectual and technical training throughout the fellowship award. I will learn key biochemical assays, ex vivo slice preparations, and will hone my skills in advanced STED super resolution microscopy. With support from this award, the training during this fellowship will provide me with the skills necessary to become a successful physician scientist.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2026 ( Subtotal = $44,942 )
2026	2026	THOMAS JEFFERSON UNIVERSITY	1020 WALNUT ST STE 1	PHILADELPHIA	PA	19107	PHILADELPHIA	USA	Extramural Research Programs in the Neurosciences and Neurological Disorders	000	4	3/20/2026	NON-COMPETING CONTINUATION	$44,942
														Subtotal = $44,942

Issue Date FY: 2025 ( Subtotal = $54,538 )
2025	2025	THOMAS JEFFERSON UNIVERSITY	1020 WALNUT ST STE 1	PHILADELPHIA	PA	19107	PHILADELPHIA	USA	Extramural Research Programs in the Neurosciences and Neurological Disorders	000	3	11/19/2024	NON-COMPETING CONTINUATION	$53,974
2025	2025	THOMAS JEFFERSON UNIVERSITY	1020 WALNUT ST STE 1	PHILADELPHIA	PA	19107	PHILADELPHIA	USA	Extramural Research Programs in the Neurosciences and Neurological Disorders	001	3	8/14/2025	NON-COMPETING CONTINUATION	$564
														Subtotal = $54,538

Issue Date FY: 2024 ( Subtotal = $50,249 )
2024	2024	THOMAS JEFFERSON UNIVERSITY	1020 WALNUT ST	PHILADELPHIA	PA	19107	PHILADELPHIA	USA	Extramural Research Programs in the Neurosciences and Neurological Disorders	001	2	5/18/2024	NON-COMPETING CONTINUATION	$1,280
2024	2024	THOMAS JEFFERSON UNIVERSITY	1020 WALNUT ST	PHILADELPHIA	PA	19107	PHILADELPHIA	USA	Extramural Research Programs in the Neurosciences and Neurological Disorders	000	2	11/9/2023	NON-COMPETING CONTINUATION	$48,969
														Subtotal = $50,249

Issue Date FY: 2023 ( Subtotal = $48,969 )
2023	2023	THOMAS JEFFERSON UNIVERSITY	1020 WALNUT ST STE 1	PHILADELPHIA	PA	19107	PHILADELPHIA	USA	Extramural Research Programs in the Neurosciences and Neurological Disorders	000	1	12/5/2022	NEW	$48,027
2023	2023	THOMAS JEFFERSON UNIVERSITY	1020 WALNUT ST STE 1	PHILADELPHIA	PA	19107	PHILADELPHIA	USA	Extramural Research Programs in the Neurosciences and Neurological Disorders	002	1	2/10/2023	NEW	$942
2023	2023	THOMAS JEFFERSON UNIVERSITY	1020 WALNUT ST STE 1	PHILADELPHIA	PA	19107	PHILADELPHIA	USA	Extramural Research Programs in the Neurosciences and Neurological Disorders	001	1	12/5/2022	NEW	$0
														Subtotal = $48,969

Grand Total All Awards = $198,698

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Determining the cellular and nanoscale localization of Synaptotagmin VII

Award Number: F30NS130973

ORGANIZATION: NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: FELLOWSHIP/SCHOLARSHIP/STUDENT LOANS

PERIOD OF PERFORMANCE START DATE: 12/05/2022

PERIOD OF PERFORMANCE END DATE: 07/31/2026

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