Wednesday, July 2, 2025 7/2/2025

SOX9 Expression Identifies A Novel Alveolar Stem Cell Population

Award Number: F30HL143911
ORGANIZATION: NATIONAL HEART, LUNG, & BLOOD INSTITUTE
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: FELLOWSHIP/SCHOLARSHIP/STUDENT LOANS
PERIOD OF PERFORMANCE START DATE: 12/01/2018
PERIOD OF PERFORMANCE END DATE: 11/30/2022

Group Awards By:

View Award Description

SOX9 Expression Identifies A Novel Alveolar Stem Cell Population - ABSTRACT While the lung has long been known to undergo extensive regeneration during adulthood, the particular stem cells involved in this process are only now being discovered. Recent studies have indicated that a subset of alveolar Type II cells, a known stem cell population of the alveoli, are preferentially activated to proliferate following different forms of injuries. Our preliminary data indicate that we have identified a novel stem cell population located in the periphery of the adult mouse lung. These cells constitute a subset of Type II cells and express SOX9, a transcription factor present in lung development but one whose expression had been previously thought to subside by adulthood. Our preliminary data show that Sox9-lineage labeled cells expand, generating new alveolar structures preferentially in the periphery of the lung lobes following partial left lobe pneumonectomy in mice. Moreover, both Sox9+ and Sox9 negative cells upregulate and re-express SOX9 in 3-dimensional organoid cultures, further suggesting that SOX9 functions to promote “stemness” in alveolar cells. Given these findings, we hypothesize that Sox9+ cells are the primary alveolar stem cell, that they preferentially proliferate to repopulate the homeostatic and post-pneumonectomy lung parenchyma, that they are necessary for these processes, and that SOX9 enhances regenerative and differentiation potential of alveolar cells. We will address these questions using a combination of lineage tracing, cell-type specific ablation, ex vivo assays, and RNA- Sequencing approaches. Aim 1 will address the contribution of Sox9+ cells to both homeostasis and compensatory lung growth following pneumonectomy. Aim 2 will determine whether Sox9+ cells are essential for maintenance of alveolar homeostasis and injury repair. Aim 3 will address whether SOX9 expression can substantially influence the stemness of alveolar Type II cells. Collectively, answering these questions will help determine whether Sox9+ cells and/or SOX9 protein are viable therapeutic targets that will help accelerate lung repair following acute injury. !


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2022 ( Subtotal = $51,752 )
2022	2022	DUKE UNIVERSITY	2200 W MAIN ST STE 710	DURHAM	NC	27708	DURHAM	USA	Lung Diseases Research	001	4	6/24/2022	NON-COMPETING CONTINUATION	$716
2022	2022	DUKE UNIVERSITY	2200 W MAIN ST STE 710	DURHAM	NC	27708	DURHAM	USA	Lung Diseases Research	000	4	11/30/2021	NON-COMPETING CONTINUATION	$51,036
														Subtotal = $51,752

Issue Date FY: 2021 ( Subtotal = $37,867 )
2021	2021	DUKE UNIVERSITY	2200 W MAIN ST STE 710	DURHAM	NC	27708	DURHAM	USA	Lung Diseases Research	001	3	3/15/2021	NON-COMPETING CONTINUATION	$516
2021	2021	DUKE UNIVERSITY	2200 W MAIN ST STE 710	DURHAM	NC	27708	DURHAM	USA	Lung Diseases Research	000	3	11/24/2020	NON-COMPETING CONTINUATION	$37,351
														Subtotal = $37,867

Issue Date FY: 2020 ( Subtotal = $37,351 )
2020	2020	DUKE UNIVERSITY	2200 W MAIN ST STE 710	DURHAM	NC	27708	DURHAM	USA	Lung Diseases Research	001	2	4/13/2020	NON-COMPETING CONTINUATION	$504
2020	2020	DUKE UNIVERSITY	2200 W MAIN ST STE 710	DURHAM	NC	27708	DURHAM	USA	Lung Diseases Research	000	2	11/27/2019	NON-COMPETING CONTINUATION	$36,847
														Subtotal = $37,351

Issue Date FY: 2019 ( Subtotal = $0 )
2019	2018	DUKE UNIVERSITY	2200 W MAIN ST STE 710	DURHAM	NC	27708	DURHAM	USA	Lung Diseases Research	000	1	12/6/2018	NEW	$0
2019	2018	DUKE UNIVERSITY	2200 W MAIN ST STE 710	DURHAM	NC	27708	DURHAM	USA	Lung Diseases Research	001	1	12/6/2018	NEW	$0
														Subtotal = $0

Issue Date FY: 2018 ( Subtotal = $49,524 )
2018	2018	DUKE UNIVERSITY	2200 W MAIN ST STE 710	DURHAM	NC	27708	DURHAM	USA	Lung Diseases Research	000	1	9/3/2018	NEW	$49,524
														Subtotal = $49,524

Grand Total All Awards = $176,494

Top

All Categories

About

Search

Reports

Data Submission

Award Information

SOX9 Expression Identifies A Novel Alveolar Stem Cell Population

Award Number: F30HL143911

ORGANIZATION: NATIONAL HEART, LUNG, & BLOOD INSTITUTE

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: FELLOWSHIP/SCHOLARSHIP/STUDENT LOANS

PERIOD OF PERFORMANCE START DATE: 12/01/2018

PERIOD OF PERFORMANCE END DATE: 11/30/2022

Federal Websites

Department of Health & Human Services

HHS Operating Divisions

HHS Staff Divisions

Download A Document Viewer