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De novo production of new knockout mouse lines for the next phase of the KOMP2 Project

Award Number: UM1OD023221
ORGANIZATION: OFFICE OF THE DIRECTOR, NIH
OPDIV: NIH
AWARD CLASS: COOPERATIVE AGREEMENT
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

Group Awards By Issue Date FY or Funding FY:

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De novo production of new knockout mouse lines for the next phase of the KOMP2 Project UM1OD023221 Administrative Supplement August 1, 2021 – July 31, 2022 ABSTRACT The aim of this administrative supplement request is to generate 25 new homozygous viable gene knockout mouse lines ready for production of male and female cohorts for phenotyping in a forthcoming KOMP2-Phase3 project. To do so, this supplement will fund production activities under 3 specific aims. Specific Aim 1 involves gene selection and exdel KO allele design, CRISPR guide in vitro quality control (QC), zygote electroporation, embryo transfer, gestation and parturition, founder (F0) screening by gene specific PCR genotyping, weaning and housing F0 mice until breeding age, F0 breeding and sequence quality control (QC) to confirm germline transmission (GLT) for 25 genes (18 at UC Davis, 7 at TCP). Specific Aim 2 involves QC of N1 mice, production reporting to GenTar, and line expansion of N2 mice to breeding age which will be ready to generate male and female cohorts for phenotyping. Specific Aim 3 involves sperm cryopreservation of 2 heterozygote males for each line produced and deposition into the MMRRC at UC Davis. This work will be completed by July 31, 2022.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2024 ( Subtotal = $0 )
2024	2023	UNIVERSITY OF CALIFORNIA, DAVIS	1850 RESEARCH PARK DR STE 300	DAVIS	CA	95618	YOLO	USA	Research Infrastructure Programs	000	12	4/10/2024	NON-COMPETING CONTINUATION	$0
														Subtotal = $0

Issue Date FY: 2023 ( Subtotal = $2,365,000 )
2023	2023	UNIVERSITY OF CALIFORNIA, DAVIS	1850 RESEARCH PARK DR, STE 300	DAVIS	CA	95618	YOLO	USA	Research Infrastructure Programs	004	12	8/7/2023	NON-COMPETING CONTINUATION	$2,365,000
2023	2022	UNIVERSITY OF CALIFORNIA, DAVIS	1850 RESEARCH PARK DR, STE 300	DAVIS	CA	95618	YOLO	USA	Research Infrastructure Programs	003	11	3/18/2023	COMPETING CONTINUATION	$0
2023	2022	UNIVERSITY OF CALIFORNIA, DAVIS	1850 RESEARCH PARK DR, STE 300	DAVIS	CA	95618	YOLO	USA	Research Infrastructure Programs	002	11	3/10/2023	COMPETING CONTINUATION	$316,106
2023	2021	UNIVERSITY OF CALIFORNIA, DAVIS	1850 RESEARCH PARK DR, STE 300	DAVIS	CA	95618	YOLO	USA	Research Infrastructure Programs	000	10	3/10/2023	SUPPLEMENT FOR EXPANSION	-$296,291
2023	2021	UNIVERSITY OF CALIFORNIA, DAVIS	1850 RESEARCH PARK DR, STE 300	DAVIS	CA	95618	YOLO	USA	Research Infrastructure Programs	001	10	3/10/2023	SUPPLEMENT FOR EXPANSION	-$19,816
														Subtotal = $2,365,000

Issue Date FY: 2022 ( Subtotal = $2,725,667 )
2022	2022	UNIVERSITY OF CALIFORNIA, DAVIS	1850 RESEARCH PARK DR, STE 300	DAVIS	CA	95618	YOLO	USA	Research Infrastructure Programs	000	11	8/12/2022	COMPETING CONTINUATION	$2,725,667
														Subtotal = $2,725,667

Issue Date FY: 2021 ( Subtotal = $2,551,619 )
2021	2021	UNIVERSITY OF CALIFORNIA, DAVIS	1850 RESEARCH PARK DR, STE 300	DAVIS	CA	95618	YOLO	USA	Research Infrastructure Programs	002	10	7/21/2021	SUPPLEMENT FOR EXPANSION	$2,391,666
2021	2021	UNIVERSITY OF CALIFORNIA, DAVIS	1850 RESEARCH PARK DR, STE 300	DAVIS	CA	95618	YOLO	USA	Research Infrastructure Programs	003	10	7/28/2021	SUPPLEMENT FOR EXPANSION	$159,953
2021	2020	UNIVERSITY OF CALIFORNIA, DAVIS	1850 RESEARCH PARK DR, STE 300	DAVIS	CA	95618	YOLO	USA	Research Infrastructure Programs	000	10	3/16/2021	NON-COMPETING CONTINUATION	$0
2021	2020	UNIVERSITY OF CALIFORNIA, DAVIS	1850 RESEARCH PARK DR, STE 300	DAVIS	CA	95618	YOLO	USA	Research Infrastructure Programs	001	10	7/19/2021	NON-COMPETING CONTINUATION	$0
														Subtotal = $2,551,619

Issue Date FY: 2020 ( Subtotal = $6,129,573 )
2020	2020	UNIVERSITY OF CALIFORNIA, DAVIS	1850 RESEARCH PARK DR, STE 300	DAVIS	CA	95618	YOLO	USA	Trans-NIH Research Support	001	9	4/7/2020	SUPPLEMENT FOR EXPANSION	$71,152
2020	2020	UNIVERSITY OF CALIFORNIA, DAVIS	1850 RESEARCH PARK DR, STE 300	DAVIS	CA	95618	YOLO	USA	Research Infrastructure Programs	002	10	7/23/2020	NON-COMPETING CONTINUATION	$6,058,421
2020	2019	UNIVERSITY OF CALIFORNIA, DAVIS	1850 RESEARCH PARK DR, STE 300	DAVIS	CA	95618	YOLO	USA	Research Infrastructure Programs	000	9	3/19/2020	NON-COMPETING CONTINUATION	$0
														Subtotal = $6,129,573

Issue Date FY: 2019 ( Subtotal = $6,658,702 )
2019	2019	UNIVERSITY OF CALIFORNIA, DAVIS	1850 RESEARCH PARK DR, STE 300	DAVIS	CA	95618	YOLO	USA	Research Infrastructure Programs	003	9	8/22/2019	SUPPLEMENT FOR EXPANSION	$600,000
2019	2019	UNIVERSITY OF CALIFORNIA, DAVIS	1850 RESEARCH PARK DR, STE 300	DAVIS	CA	95618	YOLO	USA	Research Infrastructure Programs	002	9	7/23/2019	NON-COMPETING CONTINUATION	$6,058,702
2019	2018	UNIVERSITY OF CALIFORNIA, DAVIS	1850 RESEARCH PARK DR, STE 300	DAVIS	CA	95618	YOLO	USA	Research Infrastructure Programs	001	8	3/19/2019	NON-COMPETING CONTINUATION	$0
2019	2018	UNIVERSITY OF CALIFORNIA, DAVIS	1850 RESEARCH PARK DR, STE 300	DAVIS	CA	95618	YOLO	USA	Research Infrastructure Programs	000	8	10/30/2018	SUPPLEMENT FOR EXPANSION	$0
														Subtotal = $6,658,702

Issue Date FY: 2018 ( Subtotal = $6,059,352 )
2018	2018	UNIVERSITY OF CALIFORNIA, DAVIS	1850 RESEARCH PARK DR, STE 300	DAVIS	CA	95618	YOLO	USA	Research Infrastructure Programs	001	8	8/27/2018	SUPPLEMENT FOR EXPANSION	$199,305
2018	2018	UNIVERSITY OF CALIFORNIA, DAVIS	1850 RESEARCH PARK DR, STE 300	DAVIS	CA	95618	YOLO	USA	Research Infrastructure Programs	000	8	8/1/2018	NON-COMPETING CONTINUATION	$5,860,047
														Subtotal = $6,059,352

Issue Date FY: 2017 ( Subtotal = $6,735,199 )
2017	2017	UNIVERSITY OF CALIFORNIA, DAVIS	1850 RESEARCH PARK DR, STE 300	DAVIS	CA	95618	YOLO	USA	Trans-NIH Research Support	002	7	9/13/2017	SUPPLEMENT FOR EXPANSION	$759,937
2017	2017	UNIVERSITY OF CALIFORNIA, DAVIS	1850 RESEARCH PARK DR, STE 300	DAVIS	CA	95618	YOLO	USA	Research Infrastructure Programs	000	6	3/29/2017	SUPPLEMENT FOR EXPANSION	$131,465
2017	2017	UNIVERSITY OF CALIFORNIA, DAVIS	1850 RESEARCH PARK DR, STE 300	DAVIS	CA	95618	YOLO	USA	Research Infrastructure Programs	001	7	7/27/2017	NON-COMPETING CONTINUATION	$5,843,797
														Subtotal = $6,735,199

Issue Date FY: 2016 ( Subtotal = $4,860,047 )
2016	2016	UNIVERSITY OF CALIFORNIA	1850 RESEARCH PARK DRIVE	DAVIS	CA	95618	YOLO	USA	Research Infrastructure Programs	000	6	9/8/2016	CHANGE OF GRANTEE / TRAINING INSTITUTION / AWARDING INSTITUTION	$4,860,047
														Subtotal = $4,860,047

Grand Total All Awards = $38,085,159

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De novo production of new knockout mouse lines for the next phase of the KOMP2 Project

Award Number: UM1OD023221

ORGANIZATION: OFFICE OF THE DIRECTOR, NIH

OPDIV: NIH

AWARD CLASS: COOPERATIVE AGREEMENT

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

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