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Genetic access to cortical cell types with epigenetic assays and high-throughput, low-cost enhancer screening

Award Number: UG3MH123394
ORGANIZATION: NATIONAL INSTITUTE OF MENTAL HEALTH
OPDIV: NIH
AWARD CLASS: COOPERATIVE AGREEMENT
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

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Project Summary The function of the nervous system is dependent on complex interactions between networks of neurons composed of multiple neuron types. Understanding how these networks function both in health and disease is dependent on understanding the precise connectivity between specific neuron types and their functional interactions in the intact brain. It is therefore apparent that, in order to have an adequate understanding of the nervous system, it is necessary to have detailed descriptions of neuronal connectivity with the same level of precision at which these systems operate and to selectively manipulate and measure the activity of specific cell types in the context of the normal functioning network. Such studies would be greatly facilitated by the ability to target gene expression to specific cell types in the context of AAV vectors. The research proposed here is aimed at developing and using a novel high throughput strategy and PCR-activated cell sorting for identification of cell type specific enhancer elements. The approach used will identify enhancers that can drive transgene expression in specific types of cortical neurons. Further studies will valdiate these enhancers in a real-use context for ability to facilitate imaging with genetically-encoded calcium indicators and to facilitate optogenetic inactivation. These reagents will allow future studies testing the functional contributions of specific inhibitory neuron types to perception,cognition and behavior.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2023 ( Subtotal = $0 )
2023	2022	SALK INSTITUTE FOR BIOLOGICAL STUDIES, SAN DIEGO, CALIFORNIA, THE	10010 N TORREY PINES RD	LA JOLLA	CA	92037	SAN DIEGO	USA	Mental Health Research Grants	000	3	8/8/2023	NON-COMPETING CONTINUATION	$0
														Subtotal = $0

Issue Date FY: 2022 ( Subtotal = $731,932 )
2022	2022	SALK INSTITUTE FOR BIOLOGICAL STUDIES, SAN DIEGO, CALIFORNIA, THE	10010 N TORREY PINES RD	LA JOLLA	CA	92037	SAN DIEGO	USA	Mental Health Research Grants	000	3	7/1/2022	NON-COMPETING CONTINUATION	$731,932
														Subtotal = $731,932

Issue Date FY: 2021 ( Subtotal = $731,932 )
2021	2021	SALK INSTITUTE FOR BIOLOGICAL STUDIES, SAN DIEGO, CALIFORNIA, THE	10010 N TORREY PINES RD	LA JOLLA	CA	92037	SAN DIEGO	USA	Mental Health Research Grants	000	2	8/4/2021	NON-COMPETING CONTINUATION	$731,932
														Subtotal = $731,932

Issue Date FY: 2020 ( Subtotal = $789,827 )
2020	2020	SALK INSTITUTE FOR BIOLOGICAL STUDIES, SAN DIEGO, CALIFORNIA, THE	10010 N TORREY PINES RD	LA JOLLA	CA	92037	SAN DIEGO	USA	Mental Health Research Grants	000	1	8/12/2020	NEW	$789,827
														Subtotal = $789,827

Grand Total All Awards = $2,253,691

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Genetic access to cortical cell types with epigenetic assays and high-throughput, low-cost enhancer screening

Award Number: UG3MH123394

ORGANIZATION: NATIONAL INSTITUTE OF MENTAL HEALTH

OPDIV: NIH

AWARD CLASS: COOPERATIVE AGREEMENT

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

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