PROJECT SUMMARY (Changes from awarded parent grant in bold)
The AAV BRAIN Safe and Effective Neuromodulator and Sensor Utilization across Species (SENSUS, led by
Caltech) of the NIH BRAIN Initiative Armamentarium Project will develop, validate, and disseminate integrated
(capsid and genome) engineered adeno-associated virus (AAV) tools to monitor and manipulate molecularly
defined neuronal cell types across vertebrate species. To broaden access to these reagents within the
neuroscientific community, we propose a close partnership between the AAV BRAIN SENSUS and California
State Polytechnic University, Pomona (CPP), which will host a new Armamentarium Vector Core (ArmVC). This
collaboration will also increase access to the research community itself for underrepresented minorities (URM)
and minority-serving institutions (MSI), as CPP is a designated Hispanic-Serving Institution that also serves large
populations of Pell-eligible, first-generation, and African American students. This partnership will foster close
interfacing between experts in AAV engineering for neuroscience (PI Gradinaru), AAV production innovations
and dissemination (PI Shay), and URM undergraduate research and mentoring (Co-I Steele) to build the physical
and human infrastructure for AAV production, validation, and dissemination at CPP while comprehensively
integrating MSI undergraduates.
Caltech’s CLOVER Center (directed by PI Shay) will utilize their experience in running a successful AAV
dissemination center to work with Co-I Steele and Damien Wolfe, M.S., a CPP and Steele lab alum and CLOVER
technician who will work at ArmVC, to establish robust AAV production and dissemination capabilities in the
ArmVC at CPP (Aim 1). CLOVER and ArmVC will cooperatively run a new AAV BRAIN SENSUS Program for
Inclusive Research Experience (ASPIRE) wherein CPP undergraduate students chosen as ASPIRE Scientists
will produce validated AAV for dissemination to the neuroscience community as part of the new ArmVC (Aim 2)
and work in Co-I Steele’s lab to independently validate, using immunohistochemistry, the novel AAV identified
via high-throughput methods in PI Gradinaru’s lab (Aim 3). ASPIRE Scientists will benefit from activities including
workshops, poster presentations, research seminars, and career-focused mentoring meetings with PI Gradinaru
at CPP and Caltech.
Yearly training (and re-training of returning staff and undergraduates) in AAV production by CLOVER
staff will ensure that methodological advances in AAV production, purification, and characterization developed
or adopted by the CLOVER center are quickly transferred between sites. ArmVC offerings will be publicized and
disseminated through a stand-alone website while also being integrated into AAV BRAIN SENSUS electronic
resources for seamless reagent cataloguing and feedback. With strong quality control and quality assurance
processes in place and overseen by CLOVER, the ArmVC at CPP will broaden access to modern neuroscientific
tools and help bridge the gap in URM career progression in research science.
