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Building next-generation sequencing (NGS) capacity for One Health and antimicrobial stewardship projects An on-going case study of multidrug-resistant Salmonella in zoo mammals

Award Number: U18FD008355
ORGANIZATION: FOOD AND DRUG ADMINISTRATION
OPDIV: FDA
AWARD CLASS: COOPERATIVE AGREEMENT
AWARD ACTIVITY TYPE: KDA (KNOWLEDGE/DEVELOPMENT/APPLICATION)
PERIOD OF PERFORMANCE START DATE: 09/01/2024
PERIOD OF PERFORMANCE END DATE: 08/31/2025

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Building next-generation sequencing (NGS) capacity for One Health and antimicrobial stewardship projects An on-going case study of multidrug-resistant Salmonella in zoo mammals - SUMMARY The overall goal of this project is to build next-generation sequencing (NGS) capacity that will support One Health and antimicrobial stewardship. Currently, the NDSU VDL along with multiple partners representing North Dakota zoos, human health lab, CDC, FDA, Vet-LIRN, NVSL, and USDA are investigating a multidrug-resistant (MDR) Salmonella serovar Newport in zoo mammals that clusters with a known human pathogenic strain, REPJJP01, making it a One Health issue. Implementation of the MIRO CANVAS would allow the NDSU VDL to more rapidly and cost effectively prepare NGS libraries for sequencing rather than waiting to batch samples. Additionally, the MIRO CANVAS coupled with a laptop, small sequencer, portable power supply, pipettes, minimal supplies, and small cooler bag could provide a SLAB, or Sequencing Lab-in- A-Bag, that could be extremely versatile and deployable in the field or point-of-care site. A zoological facility has agreed to help in the proof-of-concept of the SLAB. This equipment proposal has two specific aims. 1) Purchase and implement a MIRO CANVAS digital microfluidics platform for walk-away next-generation sequencing (NGS) library preparation to sequence bacterial and viral pathogens, especially Salmonella isolates. 2) Prepare a portable sequencing lab-in-a-bag or “SLAB” that can be deployed to the field or point-of-care site to detect and identify potential pathogens relatively rapidly and on-site.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2026 ( Subtotal = $0 )
2026	2024	NORTH DAKOTA STATE UNIVERSITY	1340 ADMINISTRATION AVE	FARGO	ND	58105	CASS	USA	Food and Drug Administration Research	000	1	1/8/2026	NEW	$0
														Subtotal = $0

Issue Date FY: 2024 ( Subtotal = $44,320 )
2024	2024	NORTH DAKOTA STATE UNIVERSITY	1301 12TH AVE N	FARGO	ND	58102	CASS	USA	Food and Drug Administration Research	000	1	7/31/2024	NEW	$44,320
														Subtotal = $44,320

Grand Total All Awards = $44,320

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Building next-generation sequencing (NGS) capacity for One Health and antimicrobial stewardship projects An on-going case study of multidrug-resistant Salmonella in zoo mammals

Award Number: U18FD008355

ORGANIZATION: FOOD AND DRUG ADMINISTRATION

OPDIV: FDA

AWARD CLASS: COOPERATIVE AGREEMENT

AWARD ACTIVITY TYPE: KDA (KNOWLEDGE/DEVELOPMENT/APPLICATION)

PERIOD OF PERFORMANCE START DATE: 09/01/2024

PERIOD OF PERFORMANCE END DATE: 08/31/2025

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