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Multiplex Mass Spectrometric Protein Assays for Precise Monitoring of the Pathophysiology of Obesity

Award Number: U01DK124020
ORGANIZATION: NATIONAL INSTITUTE OF DIABETES & DIGESTIVE & KIDNEY DISEASES
OPDIV: NIH
AWARD CLASS: COOPERATIVE AGREEMENT
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

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Multiplex Mass Spectrometric Protein Assays for Precise Monitoring of the Pathophysiology of Obesity - PROJECT SUMMARY/ABSTRACT The prevalence of overweight and obesity has increased dramatically in recent decades in both children and adults, reaching an epidemic proportion. In order to gain new insights into the pathophysiology of obesity towards better personalized patient care, there is a critical need to develop reliable multiplex protein assays that can be easily implemented in clinical laboratories to enable precise monitoring of many hormones and inflammatory markers closely associated with obesity. Unfortunately, current clinical assays often lack of reproducibility, especially between different clinical laboratories due to the lack of assay standardization. Targeted mass spectrometry is a promising technology for providing robust multiplex assays for blood proteins. Therefore, the overall objective of this application is to develop and validate reproducible and transferable mass spectrometry-based multiplex protein assays for enabling precise quantification of a panel of obesity markers. The panel will cover multiple aspects of obesity pathophysiology, including glucose homeostasis and energy balance (e.g., insulin, leptin, and other hormones), pro-inflammatory markers (e.g., C-reactive protein), and anti-inflammatory markers (e.g., adiponectin). To facilitate full validation of the robustness and transferability of the assays, an inter-lab assay validation will be pursued. Specifically, Aim 1 will be focused on initial assay development for optimal configurations and on demonstrating confident multiplex detection of endogenous analytes in blood plasma. Aim 2 will be centered on assay optimization and full assay characterization in the aspects of reproducibility, stability, selectivity, linearity, and limit of quantification. Aim 3 will demonstrate the utility of the assays through inter-lab quantification of endogenous analytes in a pilot cohort of clinical plasma samples from normal weight and obese subjects, and obese individual before and after weight loss and benchmarking against well-established immunoassays for selected analytes such as insulin and leptin. To facilitate the easy- implementation of the validated assays in other laboratories, all assay data including chromatographic data and detailed standard operating procedures will be shared through public data repositories and assay portals. Together, the project will establish robust and easy-to-transfer multiplex assays that will enable precise monitoring of the pathophysiology of obesity.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2025 ( Subtotal = -$11,551 )
2025	2022	BATTELLE MEMORIAL INSTITUTE	902 BATTELLE BLVD	RICHLAND	WA	99354	BENTON	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	000	4	12/13/2024	NON-COMPETING CONTINUATION	-$11,551
														Subtotal = -$11,551

Issue Date FY: 2023 ( Subtotal = $0 )
2023	2022	BATTELLE MEMORIAL INSTITUTE	902 BATTELLE BLVD	RICHLAND	WA	99354	BENTON	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	000	4	3/16/2023	NON-COMPETING CONTINUATION	$0
														Subtotal = $0

Issue Date FY: 2022 ( Subtotal = $743,932 )
2022	2022	BATTELLE MEMORIAL INSTITUTE	900 BATTELLE BLVD	RICHLAND	WA	99352	BENTON	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	001	4	7/18/2022	NON-COMPETING CONTINUATION	$743,932
2022	2021	BATTELLE MEMORIAL INSTITUTE	900 BATTELLE BLVD	RICHLAND	WA	99352	BENTON	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	000	3	2/9/2022	NON-COMPETING CONTINUATION	$0
														Subtotal = $743,932

Issue Date FY: 2021 ( Subtotal = $752,834 )
2021	2021	BATTELLE MEMORIAL INSTITUTE	900 BATTELLE BLVD	RICHLAND	WA	99352	BENTON	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	001	3	7/9/2021	NON-COMPETING CONTINUATION	$752,834
2021	2020	BATTELLE MEMORIAL INSTITUTE	900 BATTELLE BLVD	RICHLAND	WA	99352	BENTON	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	000	2	4/30/2021	NON-COMPETING CONTINUATION	$0
														Subtotal = $752,834

Issue Date FY: 2020 ( Subtotal = $755,569 )
2020	2020	BATTELLE MEMORIAL INSTITUTE	900 BATTELLE BLVD	RICHLAND	WA	99352	BENTON	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	001	2	8/13/2020	NON-COMPETING CONTINUATION	$755,569
2020	2019	BATTELLE MEMORIAL INSTITUTE	900 BATTELLE BLVD	RICHLAND	WA	99352	BENTON	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	000	1	11/8/2019	NEW	$0
														Subtotal = $755,569

Issue Date FY: 2019 ( Subtotal = $1,010,255 )
2019	2019	BATTELLE MEMORIAL INSTITUTE	900 BATTELLE BLVD	RICHLAND	WA	99352	BENTON	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	000	1	9/18/2019	NEW	$1,010,255
														Subtotal = $1,010,255

Grand Total All Awards = $3,251,039

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Multiplex Mass Spectrometric Protein Assays for Precise Monitoring of the Pathophysiology of Obesity

Award Number: U01DK124020

ORGANIZATION: NATIONAL INSTITUTE OF DIABETES & DIGESTIVE & KIDNEY DISEASES

OPDIV: NIH

AWARD CLASS: COOPERATIVE AGREEMENT

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

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