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SPATIAL GENE DYNAMICS IN THE FETAL MALE UROGENITAL TRACT

Award Number: U01DK070230
ORGANIZATION: NATIONAL INSTITUTE OF DIABETES & DIGESTIVE & KIDNEY DISEASES
OPDIV: NIH
AWARD CLASS: COOPERATIVE AGREEMENT
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 02/03/2005
PERIOD OF PERFORMANCE END DATE: 01/31/2010

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SPATIAL GENE DYNAMICS IN THE FETAL MALE UROGENITAL TRACT - DESCRIPTION (provided by applicant): The overall objective of this proposal is to establish a genome-wide, cell type-specific, computer-based searchable map of gene expression associated with critical periods of development of the normal fetal testis. The fetal testis plays an essential role in normal male urogenital tract development and many male urogenital tract disorders are linked to fetal testis perturbations. To understand the impact of genetic and environmental influences on testicular development, it is essential to determine normal gene expression patterns in the fetal testis during development. The goals of this proposal are to 1) define the 3-dimensional (3-D) spatial gene dynamics in relationship to structural development and hormonal balance in the developing testis and 2) identify cell-specific genes that can be used as biomarkers for key differentiation events. The following specific aims will address these goals: 1) Map global gene expression within specific regions in the developing fetal testis at critical developmental stages using laser capture microdissection and microarray analysis; 2) Quantitatively map key gene expression changes to specific regions within the developing fetal testis using laser capture microdissection and high throughput quantitative RT-PCR; 3) Identify testicular biomarkers representing cell-specific differentiation genes using in situ hybridization; 4) Develop a computer-based, 3-D model of the fetal testis incorporating the spatial and temporal expression patterns of key genes. Laser capture microdissection of select regions within the fetal testis during late gestation (gestation days 14-18) will be combined with microarray analysis and high-throughput real-time RT-PCR to characterize global gene expression. In situ hybridization will be used to identify gene expression in specific cell types. These data will be combined with a computerized structural mesh of the fetal testis to create a 3-D spatial and temporal gene expression model. This information will provide researchers with valuable tools for further investigations into normal fetal testicular development as well as the causes, treatment, and prevention of male urogenital tract disorders


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2009 ( Subtotal = $100 )
2009	2009	CHEMICAL INDUSTRY INSTITUTE OF TOXICOLOGY	POST OFFICE BOX 12137	RESCH TRIANGLE PK	NC	27709		USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	000	5	7/15/2009	NON-COMPETING CONTINUATION	$100
														Subtotal = $100

Issue Date FY: 2008 ( Subtotal = $152,550 )
2008	2008	CHEMICAL INDUSTRY INSTITUTE OF TOXICOLOGY	POST OFFICE BOX 12137	RESCH TRIANGLE PK	NC	27709		USA	Kidney Diseases, Urology and Hematology Research	000	4	6/5/2008	NON-COMPETING CONTINUATION	$152,550
														Subtotal = $152,550

Issue Date FY: 2007 ( Subtotal = $298,542 )
2007	2007	CHEMICAL INDUSTRY INSTITUTE OF TOXICOLOGY	POST OFFICE BOX 12137	RESCH TRIANGLE PK	NC	27709		USA	Kidney Diseases, Urology and Hematology Research	000	3	4/25/2007	NON-COMPETING CONTINUATION	$298,542
														Subtotal = $298,542

Issue Date FY: 2006 ( Subtotal = $506,491 )
2006	2006	CHEMICAL INDUSTRY INSTITUTE OF TOXICOLOGY	POST OFFICE BOX 12137	RESCH TRIANGLE PK	NC	27709		USA	Kidney Diseases, Urology and Hematology Research	000	2	4/20/2006	NON-COMPETING CONTINUATION	$506,491
														Subtotal = $506,491

Grand Total All Awards = $957,683

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SPATIAL GENE DYNAMICS IN THE FETAL MALE UROGENITAL TRACT

Award Number: U01DK070230

ORGANIZATION: NATIONAL INSTITUTE OF DIABETES & DIGESTIVE & KIDNEY DISEASES

OPDIV: NIH

AWARD CLASS: COOPERATIVE AGREEMENT

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 02/03/2005

PERIOD OF PERFORMANCE END DATE: 01/31/2010

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