Wednesday, January 7, 2026 1/7/2026

Investigating disseminated cancer cell clonal cooperation and immune control in dormancy and metastasis

Award Number: U01CA284085
ORGANIZATION: NATIONAL CANCER INSTITUTE
OPDIV: NIH
AWARD CLASS: COOPERATIVE AGREEMENT
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 05/21/2024
PERIOD OF PERFORMANCE END DATE: 04/30/2029

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Investigating disseminated cancer cell clonal cooperation and immune control in dormancy and metastasis - SUMMARY. We revealed that cancer cells can disseminate from early stages of cancer progression and after variable periods of dormancy they go on to form metastasis. Knowledge about how early DCCs (eDCCs) shape metastatic progression, how they remain dormant and immune evasive for years and how they get reactivated, is limited, but critically needed to develop more effective treatments. We discovered that as the primary tumor evolves genetically and grows, target organs are seeded by more genetically evolved late DCCs (L-DCCs) that can be dormant but also may carry more growth prone programs. How the first arriving eDCC and following L- DCCs crosstalk to affect immune niches in the lung for metastasis development has never been explored. Using genetic lineage tracing, protein barcoding, and single cell RNAseq (scRNAseq) we found that 79% of metastasis were derived from eDCCs and that eDCCs and L-DCCs cooperate in lungs to initiate metastasis. We also discovered that dormancy of eDCCs is driven by ZFP281 and NR2F1 and that interaction of eDCC and L-DCC downregulates NR2F1 expression to cooperatively found metastasis. Mouse models and human data suggest that immune-surveillance does not fully eliminate eDCCs, but how eDCCs evade immune targeting is unclear. To this end, we discovered that eDCCs have a low mutational burden, downregulate MHC-I compared to L- DCCs and upregulate the immune suppressors Gal-1 and TGFβ2. In Lungs with eDCCs CD4 and CD8 T cells seem to be dysfunctional, as depletion of CD8 T cells does not lead to eDCC outgrowth and treatment with anti- PD-L1 therapy does cause eDCC eradication. We also find that tissue resident alveolar macrophages (AMs) maintain dormancy of eDCCs but may contribute to immune evasion while also suppressing metastatic outgrowth. We hypothesize that eDCCs and L-DCCs cooperate to found metastasis by (a) the ability of L-DCCs to awaken dormant eDCCs via the production of inhibitors of pro-dormancy pathways and (b) eDCCs creating immune-suppressive lung niches that allow more immunogenic L-DCCs to escape detection. We will test 1) the role of clonal cooperation and metastasis founding capacity between eDCC and L-DCCs, 2) the molecular mechanisms used by DCCs to protect themselves from immune clearance and 3) how L-DCC and lung alveolar macrophage (AM) crosstalk affects eDCC reawakening and metastatic growth. Exploring these questions will provide insight into novel immune evasive mechanisms by early and late cooperating DCCs that may be targeted during dormancy or reactivation with immune targeting approaches to eradicate them. We also may discover ways to prevent cooperating early and late DCCs from founding metastasis using activators of pro-dormancy programs. Our work might explain why despite cancer cells disseminating early, removal of early stage tumors (e.g. DCIS)reduces metastatic relapse, and why PT size is a poor prognosis indicator: namely that early DCCs remain dormant unless reactivated by late DCCs that are more frequent the later a tumor is removed.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2026 ( Subtotal = $0 )
2026	2025	ALBERT EINSTEIN COLLEGE OF MEDICINE	1300 MORRIS PARK AVE	BRONX	NY	10461	BRONX	USA	Cancer Biology Research	000	2	12/4/2025	NON-COMPETING CONTINUATION	$0
														Subtotal = $0

Issue Date FY: 2025 ( Subtotal = $693,712 )
2025	2025	ALBERT EINSTEIN COLLEGE OF MEDICINE	1300 MORRIS PARK AVE	BRONX	NY	10461	BRONX	USA	Cancer Biology Research	001	2	5/22/2025	NON-COMPETING CONTINUATION	$69,370
2025	2025	ALBERT EINSTEIN COLLEGE OF MEDICINE	1300 MORRIS PARK AVE	BRONX	NY	10461	BRONX	USA	Cancer Biology Research	000	2	4/10/2025	NON-COMPETING CONTINUATION	$624,342
														Subtotal = $693,712

Issue Date FY: 2024 ( Subtotal = $715,578 )
2024	2024	ALBERT EINSTEIN COLLEGE OF MEDICINE	1300 MORRIS PARK AVE	BRONX	NY	10461	BRONX	USA	Cancer Biology Research	000	1	5/21/2024	NEW	$715,578
														Subtotal = $715,578

Grand Total All Awards = $1,409,290

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Investigating disseminated cancer cell clonal cooperation and immune control in dormancy and metastasis

Award Number: U01CA284085

ORGANIZATION: NATIONAL CANCER INSTITUTE

OPDIV: NIH

AWARD CLASS: COOPERATIVE AGREEMENT

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 05/21/2024

PERIOD OF PERFORMANCE END DATE: 04/30/2029

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