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Adapting multiplex microspheres to analyze glycosylation features by flow cytometry

Award Number: U01CA221213
ORGANIZATION: NATIONAL CANCER INSTITUTE
OPDIV: NIH
AWARD CLASS: COOPERATIVE AGREEMENT
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 08/01/2017
PERIOD OF PERFORMANCE END DATE: 07/31/2021

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Adapting multiplex microspheres to analyze glycosylation features by flow cytometry - PROJECT SUMMARY The goal of this proposal is to create a method for characterizing glycosylation features based around flow cytometry employing glycan-specific reagents (lectins, antibodies, Lectenz®) conjugated to spectrally-unique microspheres (beads) to detect glycan features. By combining the detection elements into a multiplexed array, an analysis can be obtained in minutes on a basic cytometer. The approach, which we call GlycoSense, can be thought of as a soluble 3D-analog of a 2D lectin microarray, and offers several unique and important advantages, including ease of use, statistical confidence, reproducibility, high sensitivity, and the benefits of equilibrium binding kinetics. In addition, this approach will enable G&D to leverage its proprietary experience in producing carbohydrate-sensing reagents. Because the method is rapid and cost-effective, it can be adopted by research laboratories without the need to rely exclusively on more sophisticated, and costly, MS/LC-MS based approaches that are typically only offered through core facilities or CROs. By adopting a familiar experimental platform (flow cytometry) there will be the additional advantage that the technology can be transferred readily into research groups with little or no expertise in the complex area of glycoprofiling. A GlycoSense analysis provides a quantified measure (a “glycoprint“) of the overall glycosylation of a sample in terms of the composition and linkages for key glycofeatures. There are four specific aims in this proposal. First, optimal reagents and standards will be evaluated for their performance in our method. Second, the GlycoSense data will be confirmed by traditional methods. Third, a kit and SOP will be developed and cross-validated. Fourth, to facilitate adoption in a laboratory environment, we will create a software interface that converts raw flow cytometry data into a convenient graphical format to present the glycosylation analysis. Software development will also assist in data management, standardization of protocols, detection of systematic errors, and statistical analysis. We will work with the academic labs and biopharma companies that have expressed interest in our technology to beta-test our kits.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2022 ( Subtotal = -$1 )
2022	2019	GLYCOSENSORS AND DIAGNOSTICS, LLC	111 RIVERBEND RD	ATHENS	GA	30602	CLARKE	USA	Trans-NIH Research Support	000	3	12/8/2021	NON-COMPETING CONTINUATION	-$1
														Subtotal = -$1

Issue Date FY: 2019 ( Subtotal = $278,800 )
2019	2019	GLYCOSENSORS AND DIAGNOSTICS, LLC	111 RIVERBEND RD	ATHENS	GA	30602	CLARKE	USA	Trans-NIH Research Support	001	3	7/12/2019	NON-COMPETING CONTINUATION	$278,800
2019	2018	GLYCOSENSORS AND DIAGNOSTICS, LLC	111 RIVERBEND RD	ATHENS	GA	30602	CLARKE	USA	Trans-NIH Research Support	000	2	4/30/2019	NON-COMPETING CONTINUATION	$0
														Subtotal = $278,800

Issue Date FY: 2018 ( Subtotal = $278,800 )
2018	2018	GLYCOSENSORS AND DIAGNOSTICS, LLC	111 RIVERBEND RD	ATHENS	GA	30602	CLARKE	USA	Trans-NIH Research Support	000	2	6/26/2018	NON-COMPETING CONTINUATION	$278,800
														Subtotal = $278,800

Issue Date FY: 2017 ( Subtotal = $278,800 )
2017	2017	GLYCOSENSORS AND DIAGNOSTICS, LLC	111 RIVERBEND RD	ATHENS	GA	30602	CLARKE	USA	Trans-NIH Research Support	000	1	7/21/2017	NEW	$278,800
														Subtotal = $278,800

Grand Total All Awards = $836,399

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Adapting multiplex microspheres to analyze glycosylation features by flow cytometry

Award Number: U01CA221213

ORGANIZATION: NATIONAL CANCER INSTITUTE

OPDIV: NIH

AWARD CLASS: COOPERATIVE AGREEMENT

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 08/01/2017

PERIOD OF PERFORMANCE END DATE: 07/31/2021

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