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POINT OF CARE CARD DEVICE FOR RAPID MOLECULAR DIAGNOSTICS

Award Number: U01AI082448
ORGANIZATION: NATIONAL INSTITUTE OF ALLERGY & INFECTIOUS DISEASES
OPDIV: NIH
AWARD CLASS: COOPERATIVE AGREEMENT
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 05/15/2009
PERIOD OF PERFORMANCE END DATE: 04/30/2013

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POINT OF CARE CARD DEVICE FOR RAPID MOLECULAR DIAGNOSTICS - DESCRIPTION (provided by applicant): This project will advance the development of a point-of-care (POC) molecular diagnostic device for the rapid and inexpensive detection of infectious disease-causing pathogens in nontraditional heath care settings. An existing, fully integrated microfluidic Chemistry and Reagent Device (CARD(tm)) will be optimized for detection of pathogens in sexually transmitted infections (STIs) and urinary tract infections (UTIs). The CARD(tm) is capable of automatically isolating and lysing infectious agents, then extracting and purifying DNA and/or RNA and PCR amplifying and detecting target sequences (to date, whole blood, plasma, saliva and vaginal swabs have been successfully processed). To improve the ease-of-use of the existing CARD(tm), this project will further integrate the use of stable liposomes (at least three year real time stability has been demonstrated) to increase the signal intensity and simultaneously transduce the biological signal into an electronic one. This will be accomplished by conjugating oliogonucleotide probes that are complementary to portions of the amplified target sequences, to the exterior surface of liposomes, which, in turn, will encapsulate electroactive compounds. The resulting liposome-target complexes will be subsequently magnetically immobilized within the microfluidic channels of the CARD(tm) by use of magnetic beads conjugated to other oligonucleotide probes that are complementary to another region of the target sequences. Upon lysis of the immobilized liposomes, the released electroactive compounds will be allowed to flow onto the surface of an interdigitated ultramicroelectrode array where a continuous and reversible redox reaction will generate electric current that will be directly proportional to the concentration of the analyte. This project will therefore allow sophisticated molecular diagnostics to be performed by individuals of varying degrees of training (including unskilled), since once the sample is introduced all preparative, analytical and readout functions are automatically performed.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2012 ( Subtotal = $388,898 )
2012	2012	RHEONIX INC	22 THORNWOOD DRIVE	ITHACA	NY	14850	TOMPKINS	USA	Allergy and Infectious Diseases Research	000	4	4/30/2012	NON-COMPETING CONTINUATION	$388,898
														Subtotal = $388,898

Issue Date FY: 2011 ( Subtotal = $575,039 )
2011	2011	RHEONIX INC	22 THORNWOOD DRIVE	ITHACA	NY	14850	TOMPKINS	USA	Allergy and Infectious Diseases Research	001	3	8/19/2011	NON-COMPETING CONTINUATION	$40,306
2011	2011	RHEONIX INC	22 THORNWOOD DRIVE	ITHACA	NY	14850	TOMPKINS	USA	Allergy and Infectious Diseases Research	000	3	4/19/2011	NON-COMPETING CONTINUATION	$367,810
2011	2011	RHEONIX INC	22 THORNWOOD DRIVE	ITHACA	NY	14850	TOMPKINS	USA	Allergy and Infectious Diseases Research	002	3	9/6/2011	NON-COMPETING CONTINUATION	$166,923
2011	2011	RHEONIX INC	22 THORNWOOD DRIVE	ITHACA	NY	14850	TOMPKINS	USA	Allergy and Infectious Diseases Research	003	3	9/15/2011	NON-COMPETING CONTINUATION	$0
														Subtotal = $575,039

Issue Date FY: 2010 ( Subtotal = $629,819 )
2010	2010	RHEONIX INC	22 THORNWOOD DRIVE	ITHACA	NY	14850	TOMPKINS	USA	Trans-NIH Recovery Act Research Support	001	2	8/2/2010	SUPPLEMENT FOR EXPANSION	$233,044
2010	2010	RHEONIX INC	22 THORNWOOD DRIVE	ITHACA	NY	14850	TOMPKINS	USA	Allergy and Infectious Diseases Research	000	2	3/30/2010	NON-COMPETING CONTINUATION	$396,775
														Subtotal = $629,819

Issue Date FY: 2009 ( Subtotal = $402,185 )
2009	2009	RHEONIX INC	22 THORNWOOD DRIVE	ITHACA	NY	14850	TOMPKINS	USA	Allergy and Infectious Diseases Research	000	1	5/5/2009	NEW	$406,148
2009	2009	RHEONIX INC	22 THORNWOOD DRIVE	ITHACA	NY	14850	TOMPKINS	USA	Allergy and Infectious Diseases Research	001	1	8/5/2009	NEW	-$3,963
														Subtotal = $402,185

Grand Total All Awards = $1,995,941

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POINT OF CARE CARD DEVICE FOR RAPID MOLECULAR DIAGNOSTICS

Award Number: U01AI082448

ORGANIZATION: NATIONAL INSTITUTE OF ALLERGY & INFECTIOUS DISEASES

OPDIV: NIH

AWARD CLASS: COOPERATIVE AGREEMENT

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 05/15/2009

PERIOD OF PERFORMANCE END DATE: 04/30/2013

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