Wednesday, February 5, 2025
2/5/2025
PROJECT SUMMARY/ABSTRACT We are requesting funds for a QPatch II-48 automated patch clamp system, designed for acquiring high-fidelity patch clamp data. A major feature of this system is that it is being used with primary cells such as immune cells and iPSC-derived cardiomyocytes or neurons. There are currently no such automated patch clamp systems available at NYU or within New York City readily available to the NYU investigators. A major advantage of the QPatch II is that the use of seal enhancer solutions that contains high concentrations and fluoride and calcium is not required. The instrument allows simultaneous patch clamp recordings with up to forty-eight individual cells, in increments of eight, at relatively low individual cost. End users need minimal training. The floor standing instrument is space saving with no special installation requirements, and the system is easily shared between users. The instrument will be housed in a central location in a shared electrophysiology facility named IonLab, managed by an experienced cellular electrophysiologist and shared by several NIH-funded major and other users. Ample institutional help and commitment is available, including from the NYU Grossman School of Medicine (NYUGSOM) Office of Collaborative Science (OCS) that assists with developing a business plan, support for IonLab and access to a common reservation and billing system (iLab), and the Department of Pathology who is committing to space and financial needs, including the maintenance of a service contract. The major user group spans several academic departments and represents both basic and translational research interests such as; pathology, cardiology, immunology and neurosciences. The major NIH-funded users will have protected use of the majority of the instrument time whereas other users will be able to use the remaining instrument time. The availability of this shared instrument will be unique in Manhattan. The requested QPatch II automated patch clamp instrument will support the research of several NIH funded researchers at NYUGSOM and NYU College of Dentistry.
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