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Development of a robust HIV-1 diagnostic system (HIV-eCaDI) for at-home testing: SUPPLEMENT

Award Number: R61AI181052
ORGANIZATION: NATIONAL INSTITUTE OF ALLERGY & INFECTIOUS DISEASES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 03/01/2026
PERIOD OF PERFORMANCE END DATE: 01/31/2027

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Development of a robust HIV-1 diagnostic system (HIV-eCaDI) for at-home testing: SUPPLEMENT - To develop the HIV-eCaDI device we are developing for our NIAID project R61 AI181052 “Development of a robust HIV-1 diagnostic system (HIV-eCaDI) for at-home testing” we prepare, quantify and validate HIV virions for testing as well as test that our HIV sample inactivation is effective to maintain biosafety during testing. We utilize a combination of chemiluminescent cellular cytopathology assessment for HIV-induced cell killing and absorbance-based ELISAs for p24 antigen quantification and are developing a fluorescence polarization assay for intact virion quantification. The 16-year-old Perkin-Elmer Victor X5 multimode platereader that we use heavily in our BSL-2 laboratory to perform each of these assays recently had a critical control board failure and replacement parts are no longer commercially available, which makes performance of these necessary HIV assessment assays impossible. The objective of this supplement is to correct this limitation by replacing our Victor X5 platereader with an equivalent modern Victor Nivo platereader so we can continue to quantify and validate high-quality HIV samples necessary for quantitative evaluation of the HIV-eCaDI devices.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2026 ( Subtotal = $518,496 )
2026	2026	COLORADO STATE UNIVERSITY	601 S HOWES ST	FORT COLLINS	CO	80521	LARIMER	USA	Allergy and Infectious Diseases Research	000	3	1/22/2026	NON-COMPETING CONTINUATION	$454,761
2026	2026	COLORADO STATE UNIVERSITY	601 S HOWES ST	FORT COLLINS	CO	80521	LARIMER	USA	Allergy and Infectious Diseases Research	001	3	4/22/2026	SUPPLEMENT FOR EXPANSION	$63,735
														Subtotal = $518,496

Issue Date FY: 2025 ( Subtotal = $444,760 )
2025	2025	COLORADO STATE UNIVERSITY	601 S HOWES ST	FORT COLLINS	CO	80521	LARIMER	USA	Allergy and Infectious Diseases Research	000	2	1/15/2025	NON-COMPETING CONTINUATION	$444,760
														Subtotal = $444,760

Issue Date FY: 2024 ( Subtotal = $463,877 )
2024	2024	COLORADO STATE UNIVERSITY	601 S HOWES ST	FORT COLLINS	CO	80521	LARIMER	USA	Allergy and Infectious Diseases Research	000	1	1/18/2024	NEW	$463,877
														Subtotal = $463,877

Grand Total All Awards = $1,427,133

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Development of a robust HIV-1 diagnostic system (HIV-eCaDI) for at-home testing: SUPPLEMENT

Award Number: R61AI181052

ORGANIZATION: NATIONAL INSTITUTE OF ALLERGY & INFECTIOUS DISEASES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 03/01/2026

PERIOD OF PERFORMANCE END DATE: 01/31/2027

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