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Identifying determinants of HIV-1 responsible for the nanoscale distribution and dynamics of virus assembly

Award Number: R56AI138625
ORGANIZATION: NATIONAL INSTITUTE OF ALLERGY & INFECTIOUS DISEASES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

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Identifying determinants of HIV-1 responsible for the nanoscale distribution and dynamics of virus assembly - Project Summary. Human Immunodeficiency Virus Type-1 (HIV-1) assembly mechanisms, namely the acquisition of the envelope glycoprotein (Env) into the assembling viral lattice, remains inadequately understood. The continued innovation of tools to interrogate these molecular and nanoscale assembly processes is beginning to disclose some of the basic principles. This proposal aims to build upon these innovations and quantify the requirements for Env assembly by interrogating viral and host factor determinants regulating intracellular trafficking, surface display, virus incorporation, and cell-to-cell spread. To accomplish these aims, we will adapt current superresolution and single-molecule tracking approaches developed by our laboratory to understand the three-dimensional structural organization and spatiotemporal dynamics of Env throughout assembly stages and cell-to-cell spread. Next, we will dissect the long Env cytoplasmic tail to understand which residues/regions drive Env incorporation. Finally, we propose to utilize genetic knock-out to understand the role of identified host trafficking factors in regulating the surface levels of Env. Our preliminary data supports the feasibility of our approach by demonstrating our ability to extract quantitative information regarding the nanoscale organization and single-molecule dynamics of Env in three-dimensions. These collective approaches will shed new light on HIV-1 assembly and cell-to-cell virus transfer, informing new models and approaches aimed at targeting these processes therapeutically.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2025 ( Subtotal = -$149,547 )
2025	2023	UNIVERSITY OF DENVER	2199 S UNIVERSITY BLVD RM 222	DENVER	CO	80210	DENVER	USA	Allergy and Infectious Diseases Research	000	6	1/19/2025	COMPETING CONTINUATION	-$149,547
														Subtotal = -$149,547

Issue Date FY: 2024 ( Subtotal = $0 )
2024	2023	UNIVERSITY OF DENVER	2199 S UNIVERSITY BLVD RM 222	DENVER	CO	80210	DENVER	USA	Allergy and Infectious Diseases Research	000	6	6/21/2024	COMPETING CONTINUATION	$0
														Subtotal = $0

Issue Date FY: 2023 ( Subtotal = $366,635 )
2023	2023	COLORADO SEMINARY	2199 S UNIVERSITY BLVD	DENVER	CO	80210	DENVER	USA	Allergy and Infectious Diseases Research	000	6	8/10/2023	COMPETING CONTINUATION	$366,635
														Subtotal = $366,635

Grand Total All Awards = $217,088

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Identifying determinants of HIV-1 responsible for the nanoscale distribution and dynamics of virus assembly

Award Number: R56AI138625

ORGANIZATION: NATIONAL INSTITUTE OF ALLERGY & INFECTIOUS DISEASES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

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