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Novel Sensor Integrated Proteome on Chip (SPOC) platform for evaluating kinetic parameters of protein interactions in high throughput

Award Number: R44TR004297
ORGANIZATION: NATIONAL CENTER FOR ADVANCING TRANSLATIONAL SCIENCES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

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Abstract A critical bottleneck in the field of proteomics is the inability to simultaneously and cost-effectively evaluate thousands of functional protein interactions at once to obtain kinetic data. Although conventional in-situ protein microarray technology is well developed, it uses fluorescent dye based detection that provides only qualitative or semi-quantitative information. There is currently no technology that offers label-free real-time interaction assays of full-length folded proteins in high throughput format. To address this unmet need, INanoBio is developing novel sensor integrated proteome on chip (SPOC) platform that combines in situ expression and capture of functional proteins on biosensors, to allow instant high throughput analysis of thousands of proteins in real time. In this SBIR phase II project, we propose to develop SPOC platform by integrating in situ produced protein arrays with surface plasmon resonance imaging (SPRi), by building integrated ProSPR instrument that will enable SPRi determination of both qualitative and kinetic information of protein biomolecular interactions in high throughput. We will develop, validate and apply 1000 protein SPOC SPRi sensor chips to study clinically relevant protein- protein interactions and test off-target binding of new antibodies produced in animal models against human proteins.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2023 ( Subtotal = $1,070,227 )
2023	2023	INANOBIO, INC.	7201 E HENKEL WAY STE 285	SCOTTSDALE	AZ	85255	MARICOPA	USA	National Center for Advancing Translational Sciences	000	2	8/15/2023	NON-COMPETING CONTINUATION	$1,070,227
														Subtotal = $1,070,227

Issue Date FY: 2022 ( Subtotal = $1,178,727 )
2022	2022	INANOBIO, INC.	7201 E HENKEL WAY STE 285	SCOTTSDALE	AZ	85255	MARICOPA	USA	National Center for Advancing Translational Sciences	000	1	8/12/2022	NEW	$1,178,727
														Subtotal = $1,178,727

Grand Total All Awards = $2,248,954

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Novel Sensor Integrated Proteome on Chip (SPOC) platform for evaluating kinetic parameters of protein interactions in high throughput

Award Number: R44TR004297

ORGANIZATION: NATIONAL CENTER FOR ADVANCING TRANSLATIONAL SCIENCES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

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