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Parallelized Imaging and Automated Analysis of Zebrafish Assays with a Gigapixel Microscope

Award Number: R44OD024879
ORGANIZATION: OFFICE OF THE DIRECTOR, NIH
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 07/15/2017
PERIOD OF PERFORMANCE END DATE: 11/30/2023

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Parallelized Imaging and Automated Analysis of Zebrafish Assays with a Gigapixel Microscope - Significance: High-throughput optical microscopy is currently transforming the research fields of genetics, drug discovery and neuroscience. Large-scale optical assays now routinely use thousands of high-resolution images to offer critical insights into the human body, our brain and the diseases that affect us. Today's optical microscopes and their associated image processing software, however, are still far from ideal. Current microscopes cannot form images with cellular-scale resolution over an area larger than a few square centimeters, which fundamentally limits our ability to monitor the detailed movements of living systems. For screening many in vivo model organisms, such as zebrafish, this limitation prevents current setups from simultaneously observing multiple freely moving organisms at cellular resolution, which makes high-resolution in vivo screening experiments challenging and time-consuming. It has also led to a scarcity of effective image processing software for high- resolution organism image analysis. Proposal: Over the course of successful Phase I and Phase II projects, Ramona Optics has developed a new “micro-camera array microscope” (MCAM) that overcomes the above limitations by capturing video with up to 1 gigapixel per frame, which can resolve hundreds of freely-swimming organisms at near-cellular-level detail (5 µm/pixel). In this proposal, Ramona Optics will produce a suite of software that takes full advantage of the MCAM's information-rich recordings. This software will automatically track and measure key morphological and fluorescence features across all zebrafish within a full well plate, simultaneously, to replace currently tedious and time-consuming tasks. Our MCAM and its new software will transform current toxicology and pharmacology research that relies on in vivo organism screening, by producing more experimental insights in less time and with higher accuracy and repeatability than current methods. SA1: MCAM software for parallelized larval tracking and video registration: Ramona Optics will produce Python software to track, crop and register all larvae within the MCAM FOV to produce per-larvae centered video for subsequent morphological analysis. We will demonstrate the ability to register video of hundreds of larvae imaged simultaneously (<0.1% error rate) to reduce saved data by 40X and produce standardized per-organism datasets. SA2: Automated annotation of morphological endpoints: Working with the Tanguay Lab at Oregon State University and the Yoder Lab at NC State University, we will create algorithms to automatically compute 10 larval zebrafish morphological endpoints, such as gaze direction, pectoral fin position, and body curvature, which we will verify in a series of toxicology titration experiments (validated across labs) to demonstrate at least 100X speed-up to current screening methods. SA3: Automated bright-field video analysis: We will then extend our automated morphology analysis software to bright-field video to provide a time-dependent per-organism statistical analysis. We will showcase this software by tracking eye angle across 96 larvae simultaneously to demonstrate visual acuity as a new endpoint in toxicology screens (Tanguay Lab). SA4: Enabling high-throughput fluorescence video analysis: We will extend our parallelized software to monitor localized fluorescence across all larvae within a 96 well-plate, and then test it in an immunotoxicology experiment (Yoder Lab). The outcome of this Phase IIB project will be a flagship MCAM software suite ready for deployment to all MCAM users.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2024 ( Subtotal = $0 )
2024	2022	RAMONA OPTICS INC	1007 BURCH AVE	DURHAM	NC	27701	DURHAM	USA	Research Infrastructure Programs	000	5	4/16/2024	NON-COMPETING CONTINUATION	$0
														Subtotal = $0

Issue Date FY: 2022 ( Subtotal = $973,119 )
2022	2022	RAMONA OPTICS, INC.	1007 BURCH AVE	DURHAM	NC	27701	DURHAM	USA	Research Infrastructure Programs	001	5	6/21/2022	NON-COMPETING CONTINUATION	$973,119
2022	2019	RAMONA OPTICS, INC.	1007 BURCH AVE	DURHAM	NC	27701	DURHAM	USA	Research Infrastructure Programs	000	3	11/13/2021	NON-COMPETING CONTINUATION	$0
														Subtotal = $973,119

Issue Date FY: 2021 ( Subtotal = $999,300 )
2021	2021	RAMONA OPTICS, INC.	1007 BURCH AVE	DURHAM	NC	27701	DURHAM	USA	Research Infrastructure Programs	001	4	5/27/2021	COMPETING CONTINUATION	$999,300
2021	2019	RAMONA OPTICS, INC.	1007 BURCH AVE	DURHAM	NC	27701	DURHAM	USA	Research Infrastructure Programs	000	3	5/26/2021	NON-COMPETING CONTINUATION	$0
														Subtotal = $999,300

Issue Date FY: 2019 ( Subtotal = $738,353 )
2019	2019	RAMONA OPTICS, INC.	1007 BURCH AVE	DURHAM	NC	27701	DURHAM	USA	Research Infrastructure Programs	001	3	8/20/2019	NON-COMPETING CONTINUATION	$738,353
2019	2018	RAMONA OPTICS, INC.	2514 RAMONA ST	PALO ALTO	CA	94301	SANTA CLARA	USA	Research Infrastructure Programs	000	2	12/28/2018	COMPETING CONTINUATION	$0
														Subtotal = $738,353

Issue Date FY: 2018 ( Subtotal = $760,176 )
2018	2018	RAMONA OPTICS, INC.	2514 RAMONA ST	PALO ALTO	CA	94301	SANTA CLARA	USA	Research Infrastructure Programs	000	2	9/20/2018	COMPETING CONTINUATION	$760,176
														Subtotal = $760,176

Grand Total All Awards = $3,470,948

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Parallelized Imaging and Automated Analysis of Zebrafish Assays with a Gigapixel Microscope

Award Number: R44OD024879

ORGANIZATION: OFFICE OF THE DIRECTOR, NIH

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 07/15/2017

PERIOD OF PERFORMANCE END DATE: 11/30/2023

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