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SCREENING DEVICE FOR DIFFERENTIATED PRIMARY CELL MODELS OF AIRWAY EPITHELIA

Award Number: R44HL103079
ORGANIZATION: NATIONAL HEART, LUNG, & BLOOD INSTITUTE
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 04/01/2010
PERIOD OF PERFORMANCE END DATE: 07/31/2014

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SCREENING DEVICE FOR DIFFERENTIATED PRIMARY CELL MODELS OF AIRWAY EPITHELIA - DESCRIPTION (provided by applicant): Approaches to drug screening typically involve dramatic compromises in order to achieve high throughput and hold down costs. Examples include the use of immortalized, heterologous cell lines, and genetic engineering to over-express the target protein and/or incorporate a fluorescent surrogate reporter to display the result. At least partly because such systems often produce responses that are not relevant to the actual human disease state, high throughput screening has not historically delivered a strong return on investment. More representative primary cell models are often available for tissues and diseases, but these models are underutilized in screening because of the lack of technological solutions and high costs. For similar reasons, more relevant assay technologies such as endogenous immunocytochemistry and electrophysiology are not commonly employed in HTS. The goal of this project is to bring the gold standard, organotypic cell model for airway epithelia into true high throughput screening, and enable the use of more informative high content assays including endogenous CFTR trafficking and airway surface liquid height. In addition to applications for cystic fibrosis and COPD, the resultant device will also be valuable for skin models and drug transport studies. The device consists of an array of 96 microchambers in standard microplate format. The microchamber design is small enough to be compatible with 384-well plate densities, so 384 microchambers per plate is readily achievable. The plate includes specialized features for compatibility and ease-of-use with standard liquid handling robotics, and high resolution microscopy. Importantly, the device is simple enough to be produced at a cost consistent with the cost constraints of HTS labs, and confers dramatic savings in primary cell and media consumption. The feasibility study on a small-scale device was successfully completed, indicating that the highly miniaturized approach is compatible with organotypic epithelial airway culture at air-liquid interface. Although not an aim of this proposal this device is designed to be integrated into an electrophysiology instrument designed specifically for this culture system.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2013 ( Subtotal = $194,007 )
2013	2013	BELLBROOK LABS LLC	5500 NOBEL DRIVE SUITE 250	MADISON	WI	53711	DANE	USA	Lung Diseases Research	001	3	7/22/2013	NON-COMPETING CONTINUATION	$194,007
2013	2012	BELLBROOK LABS LLC	5500 NOBEL DRIVE SUITE 250	MADISON	WI	53711	DANE	USA	Lung Diseases Research	000	2	5/22/2013	COMPETING CONTINUATION	$0
														Subtotal = $194,007

Issue Date FY: 2012 ( Subtotal = $539,579 )
2012	2012	BELLBROOK LABS LLC	5500 NOBEL DRIVE SUITE 250	MADISON	WI	53711	DANE	USA	Lung Diseases Research	000	2	7/23/2012	COMPETING CONTINUATION	$539,579
														Subtotal = $539,579

Grand Total All Awards = $733,586

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SCREENING DEVICE FOR DIFFERENTIATED PRIMARY CELL MODELS OF AIRWAY EPITHELIA

Award Number: R44HL103079

ORGANIZATION: NATIONAL HEART, LUNG, & BLOOD INSTITUTE

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 04/01/2010

PERIOD OF PERFORMANCE END DATE: 07/31/2014

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