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DNA-optical barcoding technology for spatiotemporal single-cell analysis

Award Number: R44HG013591
ORGANIZATION: NATIONAL HUMAN GENOME RESEARCH INSTITUTE
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 02/20/2024
PERIOD OF PERFORMANCE END DATE: 10/19/2024

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DNA-optical barcoding technology for spatiotemporal single-cell analysis - Abstract Combining live imaging with single-cell sequencing is a powerful approach for gaining comprehensive understanding of individual cell behaviors within complex biological systems. However, existing approaches are limited in throughput, rely on computational data alignments that are prone to bias, or require analysis of isolated cells. The goal of this Fast-Track SBIR application is to develop a novel cellular barcoding technology that will enable researchers to conduct both standard live-cell imaging and high- throughput single-cell sequencing, and then align the two sets of data unambiguously according to the cell barcodes. The proposed approach uses dual-barcoded laser particles (LPs) with unique pairs of optical emission spectra and oligonucleotide sequences. These barcodes can be identified either optically during imaging or by single-cell sequencing. Built on promising preliminary data, Phase I of this project aims to demonstrate the feasibility of dual-barcoded LPs on a small scale. Upon success of Phase I, Phase II will streamline and scale up the fabrication of the LPs and test and validate the technology towards commercialization. The optical barcode readout requires a high-resolution spectrometer and a pump laser. In Phase II, add-on hardware modules and cell-tracking software will be developed and tested to be compatible with existing commercial microscopes, making it easier for researchers to adopt the technology. Additionally, Phase II research aims to demonstrate the usefulness of imaging-sequencing data via a pilot study of cancer cells. Furthermore, potential risks and solutions for the technology will be explored, including the long-term effects of LPs on cell gene expression patterns. Overall, the outcome of this Fast- Track project will be significant technological advancements to the field of single-cell analysis for both basic research and drug discovery.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2025 ( Subtotal = $900,298 )
2025	2025	LASE INNOVATION INC	335 BEAR HILL RD	WALTHAM	MA	02451	MIDDLESEX	USA	Human Genome Research	000	2	2/28/2025	EXTENSION WITH OR WITHOUT FUNDS	$900,298
2025	2024	LASE INNOVATION INC	335 BEAR HILL RD	WALTHAM	MA	02451	MIDDLESEX	USA	Human Genome Research	001	1	3/4/2025	NEW	$0
														Subtotal = $900,298

Issue Date FY: 2024 ( Subtotal = $239,680 )
2024	2024	LASE INNOVATION INC	335 BEAR HILL RD	WALTHAM	MA	02451	MIDDLESEX	USA	Human Genome Research	001	1	9/13/2024	NEW	$0
2024	2024	LASE INNOVATION INC	16 TOWER OFFICE PARK	WOBURN	MA	01801	MIDDLESEX	USA	Human Genome Research	000	1	2/20/2024	NEW	$239,680
														Subtotal = $239,680

Grand Total All Awards = $1,139,978

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DNA-optical barcoding technology for spatiotemporal single-cell analysis

Award Number: R44HG013591

ORGANIZATION: NATIONAL HUMAN GENOME RESEARCH INSTITUTE

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 02/20/2024

PERIOD OF PERFORMANCE END DATE: 10/19/2024

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