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STREAMLINED TEMPLATE PREPARATION FOR ADVANCED SEQUENCING METHODS

Award Number: R44HG003946
ORGANIZATION: NATIONAL HUMAN GENOME RESEARCH INSTITUTE
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 12/01/2005
PERIOD OF PERFORMANCE END DATE: 02/28/2010

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STREAMLINED TEMPLATE PREPARATION FOR ADVANCED SEQUENCING METHODS - DESCRIPTION (provided by applicant): The advent of next-generation sequencing technologies is allowing researchers to perform studies and make discoveries which previously were not economically or technically feasible. For example, precise knowledge of the genomic sequence within neoplastic tissues can be an indicator for treatment (similar to the erbB-2 receptor gene, Her-2, for breast cancer). Although much research and development effort has gone into the core chemistries and instruments which actually produce sequence data, few new techniques have been applied to the preparation of samples for high-throughput or use in the clinic. In particular, sequencing systems that use beads as both the solid support for amplification and to help localize samples on a sequencing chip still require tedious processing by hand. As labs scale up to use the new sequencing technologies, they are very quickly realizing that the bottleneck in the operation is in the sample preparation. In this Phase II application, we propose to take the sample preparation steps far beyond the original Phase I scope of library construction. We propose to automate the entire sample preparation process, starting just after DNA fragmentation, through library construction and ending just prior to attachment to a chip. The proposed system will be compatible with a number of next generation systems which are starting to enter the market. Public Health Relevance: This Public Health Relevance is not available.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2009 ( Subtotal = $759,811 )
2009	2009	INTELLIGENT BIO-SYSTEMS INC	25 ROLLING LANE	WESTON	MA	02493	MIDDLESEX	USA	Human Genome Research	001	3	2/23/2009	NON-COMPETING CONTINUATION	$759,811
2009	2008	INTELLIGENT BIO-SYSTEMS INC	25 ROLLING LANE	WESTON	MA	02493	MIDDLESEX	USA	Human Genome Research	000	2	2/13/2009	COMPETING CONTINUATION	$0
														Subtotal = $759,811

Issue Date FY: 2008 ( Subtotal = $712,037 )
2008	2008	INTELLIGENT BIO-SYSTEMS INC	25 ROLLING LANE	WESTON	MA	02493	MIDDLESEX	USA	Human Genome Research	000	2	5/13/2008	COMPETING CONTINUATION	$712,037
														Subtotal = $712,037

Grand Total All Awards = $1,471,848

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STREAMLINED TEMPLATE PREPARATION FOR ADVANCED SEQUENCING METHODS

Award Number: R44HG003946

ORGANIZATION: NATIONAL HUMAN GENOME RESEARCH INSTITUTE

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 12/01/2005

PERIOD OF PERFORMANCE END DATE: 02/28/2010

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