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ENHANCED LOCAL LYMPH NODE ASSAY USING FLOW CYTOMETRY

Award Number: R44ES010234
ORGANIZATION: NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 09/30/2002
PERIOD OF PERFORMANCE END DATE: 03/31/2005

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ENHANCED LOCAL LYMPH NODE ASSAY USING FLOW CYTOMETRY - DESCRIPTION (Applicant?s abstract): Although the murine Local Lymph Node Assay (LLNA) is effective at detecting potential irritants/sensitizers, this assay, in its current standard form: 1) cannot readily differentiate some types of irritants from respiratory and contact sensitizers; 2) utilizes moderate amounts of radioactive material and; 3) requires increased numbers of animals to determine the different endpoints (i.e., proliferation, immunophenotype) to fully characterize the response of an animal to a topically-applied chemical. To enhance and improve this important test, our company has implemented several innovative modifications to the standard LLNA. We have applied flow cytometric techniques to this assay to increase the sensitivity/specificity of the assay, eliminate the use of radioactive material and decrease the number of animals used for screening. It has been determined by the results of SBIR Phase I studies that the proposed enhanced flow cytometry-based LLNA is technically and commercially feasible. However, to develop this assay to its full commercial potential, which our company would provide to the chemical, pharmaceutical and consumer products industries, our group must: 1) validate the enhanced version of the LLNA; 2) establish the cytometry-based LLNA as a GLP compliant assay at our company and; 3) develop and provide various versions of the enhanced LLNA for our clients.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2004 ( Subtotal = $266,597 )
2004	2004	MB RESEARCH LABORATORIES	1765 WENTZ ROAD	SPINNERSTOWN	PA	18968	BUCKS	USA	Applied Toxicological Research and Testing	000	4	3/31/2004	NON-COMPETING CONTINUATION	$266,597
														Subtotal = $266,597

Issue Date FY: 2003 ( Subtotal = $546,730 )
2003	2003	MB RESEARCH LABORATORIES	1765 WENTZ ROAD	SPINNERSTOWN	PA	18968	BUCKS	USA	Applied Toxicological Research and Testing	000	3	6/13/2003	NON-COMPETING CONTINUATION	$546,730
														Subtotal = $546,730

Issue Date FY: 2002 ( Subtotal = $313,397 )
2002	2002	MB RESEARCH LABORATORIES	1765 WENTZ ROAD	SPINNERSTOWN	PA	18968	BUCKS	USA	Applied Toxicological Research and Testing	000	2	9/21/2002	COMPETING CONTINUATION	$313,397
														Subtotal = $313,397

Grand Total All Awards = $1,126,724

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ENHANCED LOCAL LYMPH NODE ASSAY USING FLOW CYTOMETRY

Award Number: R44ES010234

ORGANIZATION: NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 09/30/2002

PERIOD OF PERFORMANCE END DATE: 03/31/2005

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