Tuesday, November 4, 2025 11/4/2025

Time-lapse Flow Cytometry for Kinetic Profiling of T-Cell Function

Award Number: R44CA281529
ORGANIZATION: NATIONAL CANCER INSTITUTE
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 09/05/2023
PERIOD OF PERFORMANCE END DATE: 08/31/2025

Group Awards By:

View Award Description

Time-lapse Flow Cytometry for Kinetic Profiling of T-Cell Function - Abstract Despite progress in immunotherapies, there are significant challenges to overcome to make them more broadly applicable to different cancers and more effective for patients. However, current efforts to improve adoptive cell therapies and immune checkpoint blockade as well as cancer vaccines are hampered by the lack of a method to characterize the functional and phenotypical changes of different subpopulations of T cells over time at high throughput. The goal of this SBIR Phase II project is to develop a novel flow-cytometry method that can solve this technological bottleneck. The time-lapse flow cytometry uses laser particle (LP) barcodes to track each cell across flow measurements taken at different time points. Built on demonstrated proof-of-concept data, this project is focused on establishing a set of novel assays for characterizing T cells in response to different stimuli in a time-resolved manner. The first specific aim is to demonstrate short-term time-lapse assays to profile cytokine secretion (t-SEC). Secretion of TNF, IFNγ and IL10 from millions of the same cells in response to different types of stimulation are measured at multiple timepoints over 24 hours. The second specific aim is to demonstrate long-term time-lapse assay to monitor phenotype (t-PHENO), including T-cell exhaustion over 10 days. The third specific aim is to validate the t-SEC and t-PHENO assays with patient samples. The time- resolved, high-throughput, high-parameter assays are expected to accelerate the development of more effective and durable immunotherapies for cancer (>$100 B global market). Beyond immunotherapy, time-lapse flow cytometry is also expected to be useful in fundamental immunological research and vaccine development.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2024 ( Subtotal = $837,126 )
2024	2024	LASE INNOVATION INC	335 BEAR HILL RD	WALTHAM	MA	02451	MIDDLESEX	USA	Cancer Biology Research	000	2	7/26/2024	NON-COMPETING CONTINUATION	$837,126
														Subtotal = $837,126

Issue Date FY: 2023 ( Subtotal = $1,002,340 )
2023	2023	LASE INNOVATION INC	16 TOWER OFFICE PARK	WOBURN	MA	01801	MIDDLESEX	USA	Cancer Biology Research	000	1	9/5/2023	NEW	$1,002,340
														Subtotal = $1,002,340

Grand Total All Awards = $1,839,466

Top

All Categories

About

Search

Reports

Data Submission

Award Information

Time-lapse Flow Cytometry for Kinetic Profiling of T-Cell Function

Award Number: R44CA281529

ORGANIZATION: NATIONAL CANCER INSTITUTE

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 09/05/2023

PERIOD OF PERFORMANCE END DATE: 08/31/2025

Federal Websites

Department of Health & Human Services

HHS Operating Divisions

HHS Staff Divisions

Download A Document Viewer