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Spatial and single cell analysis of tumor microenvironment by seqFISH+

Award Number: R44CA247353
ORGANIZATION: NATIONAL CANCER INSTITUTE
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 04/01/2020
PERIOD OF PERFORMANCE END DATE: 03/31/2022

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Spatial and single cell analysis of tumor microenvironment by seqFISH+ - SUMMARY Tumor microenvironment (TME) is a key determinant of tumor growth, disease progression and resistance to chemotherapy. There is a diverse repertoire of distinct cell type that are found in the tumor microenvironment, with many that have not been fully enumerated. In addition, little is known about the “geography” of the tumors and the spatial organization of the distinct cell types. There is therefore, an immediate need of transformative tools to enable in-depth understanding of the TME in many forms of malignancies. Spatial Genomics Inc is commercializing seqFISH+ (Eng et al, Nature 2019) which can achieves multiplexing of 10,000 genes at the mRNA level in single cells in tissues with spatial information preserved. Using the seqFISH+ transcriptome profiles, novel cells types in situ and spatial organizations can be directly identified and visualized in tumor tissue slices. These capabilities make seqFISH+ a powerful tool for examining the different cell types and their interactions in the tumor microenvironment. In this SBIR proposal, we will develop an instrument and the associated software and reagents that will allow researchers to perform seqFISH+ in tumor slices. In collaboration with Amgen and Children’s Hospital at LA (CHLA), we will apply seqFISH+ to image the tumor microenvironment in both mouse model systems and human pediatric neuroblastoma samples.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2022 ( Subtotal = $0 )
2022	2021	SPATIAL GENOMICS, INC.	1224 E GREEN ST #120	PASADENA	CA	91106	LOS ANGELES	USA	Cancer Biology Research	000	2	8/2/2022	NON-COMPETING CONTINUATION	$0
														Subtotal = $0

Issue Date FY: 2021 ( Subtotal = $999,999 )
2021	2021	SPATIAL GENOMICS, INC.	1224 E GREEN ST #120	PASADENA	CA	91106	LOS ANGELES	USA	Cancer Biology Research	000	2	3/12/2021	NON-COMPETING CONTINUATION	$999,999
2021	2020	SPATIAL GENOMICS, INC.	1224 E GREEN ST #120	PASADENA	CA	91106	LOS ANGELES	USA	Cancer Biology Research	001	1	3/29/2021	NEW	$0
														Subtotal = $999,999

Issue Date FY: 2020 ( Subtotal = $999,999 )
2020	2020	SPATIAL GENOMICS, INC.	1224 E GREEN ST #120	PASADENA	CA	91106	LOS ANGELES	USA	Cancer Biology Research	000	1	3/25/2020	NEW	$999,999
														Subtotal = $999,999

Grand Total All Awards = $1,999,998

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Spatial and single cell analysis of tumor microenvironment by seqFISH+

Award Number: R44CA247353

ORGANIZATION: NATIONAL CANCER INSTITUTE

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 04/01/2020

PERIOD OF PERFORMANCE END DATE: 03/31/2022

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