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In situ single cell imaging

Award Number: R44CA239939
ORGANIZATION: NATIONAL CANCER INSTITUTE
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 12/11/2019
PERIOD OF PERFORMANCE END DATE: 05/31/2023

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In situ single cell imaging - Abstract Cancer immunotherapy is revolutionizing the landscape of cancer treatment, with checkpoint inhibitors, activated T cell therapies, and a range of other combinatorial approaches providing lasting benefits for patients. However, the response rate to these advanced therapeutic approaches is only 15-30%, demonstrating the urgent need of better understanding of and eventually addressing the low-response problem, as well as the need of technologies for patient stratification, which often involves the use of biomarkers to fingerprint individuals’ tumors. Here, we propose to develop a versatile quantum dot (Qdot)- based single-cell molecular profiling technology for in situ multiplexed quantitative molecular characterization of biological specimens with optical imaging resolution. Despite the availability of Qdots commercially since early 2000s, they have not received broad adoptions and enabled biological discoveries, although they offer much better optical properties than organic fluorophores. Key problems include the modest improvement in multiplexing, and the sophisticated, lengthy, cost-prohibitive, and low-reproducibility probe preparation approaches developed to date. Based on a breakthrough made by Prof. Gao’s lab (Nat. Commun.), the technology proposed here is capable of solving both problems (patent recently awarded, ready for commercialization). It simultaneously achieves the multiplexing capability of mass spectrometry (10s-100s) and the sensitivity, resolution, in situ imaging, low cost, great accessibility (microscopes are standard compared to mass spectrometers) of immunofluorescence.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2024 ( Subtotal = $0 )
2024	2021	DOTQUANT LLC	2907 NE 196TH ST	SHORELINE	WA	98155	KING	USA	Cancer Detection and Diagnosis Research	000	3	2/1/2024	NON-COMPETING CONTINUATION	$0
														Subtotal = $0

Issue Date FY: 2023 ( Subtotal = $0 )
2023	2021	DOTQUANT LLC	2907 NE 196TH ST	SHORELINE	WA	98155	KING	USA	Cancer Detection and Diagnosis Research	000	3	11/14/2022	NON-COMPETING CONTINUATION	$0
														Subtotal = $0

Issue Date FY: 2021 ( Subtotal = $605,767 )
2021	2021	DOTQUANT LLC	2907 NE 196TH ST	SHORELINE	WA	98155	KING	USA	Cancer Detection and Diagnosis Research	000	3	12/1/2020	NON-COMPETING CONTINUATION	$605,767
2021	2021	DOTQUANT LLC	2907 NE 196TH ST	SHORELINE	WA	98155	KING	USA	Cancer Detection and Diagnosis Research	003	3	9/17/2021	NON-COMPETING CONTINUATION	$0
2021	2020	DOTQUANT LLC	2907 NE 196TH ST	SHORELINE	WA	98155	KING	USA	Cancer Detection and Diagnosis Research	002	2	3/16/2021	EXTENSION WITH OR WITHOUT FUNDS	$21,415
2021	2019	DOTQUANT LLC	2907 NE 196TH ST	SHORELINE	WA	98155	KING	USA	Cancer Detection and Diagnosis Research	001	1	3/16/2021	NEW	-$21,415
														Subtotal = $605,767

Issue Date FY: 2020 ( Subtotal = $605,767 )
2020	2020	DOTQUANT LLC	2907 NE 196TH ST	SHORELINE	WA	98155	KING	USA	Cancer Detection and Diagnosis Research	002	2	12/13/2019	EXTENSION WITH OR WITHOUT FUNDS	$39,630
2020	2020	DOTQUANT LLC	2907 NE 196TH ST	SHORELINE	WA	98155	KING	USA	Cancer Detection and Diagnosis Research	001	2	12/11/2019	EXTENSION WITH OR WITHOUT FUNDS	$566,137
2020	2019	DOTQUANT LLC	2907 NE 196TH ST	SHORELINE	WA	98155	KING	USA	Cancer Detection and Diagnosis Research	000	1	11/8/2019	NEW	$0
														Subtotal = $605,767

Issue Date FY: 2019 ( Subtotal = $216,126 )
2019	2019	DOTQUANT LLC	2907 NE 196TH ST	SHORELINE	WA	98155	KING	USA	Cancer Detection and Diagnosis Research	000	1	5/1/2019	NEW	$216,126
														Subtotal = $216,126

Grand Total All Awards = $1,427,660

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In situ single cell imaging

Award Number: R44CA239939

ORGANIZATION: NATIONAL CANCER INSTITUTE

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 12/11/2019

PERIOD OF PERFORMANCE END DATE: 05/31/2023

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