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Commercialization of cavitation-enhancing nanodroplets for DNA sample fragmentation in NGS applications

Award Number: R44CA232902
ORGANIZATION: NATIONAL CANCER INSTITUTE
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 07/05/2018
PERIOD OF PERFORMANCE END DATE: 08/31/2023

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Commercialization of cavitation-enhancing nanodroplets for DNA sample fragmentation in NGS applications - PROJECT SUMMARY The completion of the Human Genome Project has established NGS as the gold standard technology for personalized/precision medicine and diagnostics. Genetic testing kits are now as readily available as pregnancy test kits, and advances in genetic analysis, gene therapy, and genetic modification in the 21st century promise to parallel the development of transistors and integrated circuits in the 20th century. The challenge is that technology for preparing biological samples for sequencing has not maintained a similar rapid advancement to gene sequencing. Fragmentation of DNA randomly without bias is an essential, but difficult step in the preparation of genomic DNA for NGS applications. A narrow range of DNA fragment lengths is required for sequencing, and inefficient or inconsistent fragmentation will result in incomplete or excluded reads, corrupting the sequencing. Sonication, which fragments DNA through cavitation, is the gold standard for DNA fragmentation. However, our customer discover interviews have reinforced that this is a significant pain point in their sample preparation process due to its low throughput and inconsistency, particularly for industry developing large-scale sequencing efforts. We have invented a novel nanodroplet reagent that enhances cavitation from an acoustic sonicator, and greatly improves consistency and reduces time and acoustic power requirements for DNA fragmentation. Triangle Biotechnology has licensed the patents describing this cavitation enhancing technology. We have achieved milestones proposed in Phase I, demonstrating product and technical feasibility. With this SBIR Phase II, we will perform key R&D tasks, product development, and validation studies to prepare for commercialization. Success of this project will enable a novel commercial solution for addressing market pain points: substantially improving throughput and consistency of DNA fragmentation for NGS sample preparation while reducing cost.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2024 ( Subtotal = $0 )
2024	2021	TRIANGLE BIOTECHNOLOGY, INC	303 W BARBEE CHAPEL RD	CHAPEL HILL	NC	27517	ORANGE	USA	Cancer Biology Research	000	3	2/12/2024	NON-COMPETING CONTINUATION	$0
														Subtotal = $0

Issue Date FY: 2021 ( Subtotal = $1,154,747 )
2021	2021	TRIANGLE BIOTECHNOLOGY, INC.	303 W BARBEE CHAPEL RD	CHAPEL HILL	NC	27517	ORANGE	USA	Cancer Biology Research	000	3	7/30/2021	NON-COMPETING CONTINUATION	$1,154,747
														Subtotal = $1,154,747

Issue Date FY: 2020 ( Subtotal = $844,657 )
2020	2020	TRIANGLE BIOTECHNOLOGY, INC.	303 W BARBEE CHAPEL RD	CHAPEL HILL	NC	27517	ORANGE	USA	Cancer Biology Research	000	2	9/9/2020	COMPETING CONTINUATION	$844,657
														Subtotal = $844,657

Grand Total All Awards = $1,999,404

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Commercialization of cavitation-enhancing nanodroplets for DNA sample fragmentation in NGS applications

Award Number: R44CA232902

ORGANIZATION: NATIONAL CANCER INSTITUTE

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 07/05/2018

PERIOD OF PERFORMANCE END DATE: 08/31/2023

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